Tissue-specific and light regulation of a larch ribulose-1,5-bisphosphate carboxylase promoter in transgenic tobacco

1994 ◽  
Vol 24 (8) ◽  
pp. 1689-1693 ◽  
Author(s):  
Michael A. Campbell ◽  
David B. Neale ◽  
Peter Harvie ◽  
Keith W. Hutchison
Keyword(s):  
Light Regulation ◽  
Evolutionary Conservation ◽  
Gus Activity ◽  
Larix Laricina ◽  
Histochemical Analysis ◽  
Bisphosphate Carboxylase ◽  
Tissue Specific ◽  
Rbcs Promoter ◽  
Organ Specific ◽  
Root Tissues

A chimeric gene composed of an eastern larch (Larix laricina (Du Roi) K. Koch) ribulose-1,5-bisphosphate carboxylase (RbcS) promoter linked to the β-glucuronidase (GUS) coding sequence was transferred to tobacco (Nicotianatabacum (L.)) via Agrobacteriumtumefaciens transformation. Based on GUS activity the larch RbcS promoter functioned in an organ-specific and light-regulated manner. Histochemical analysis revealed high levels of GUS activity in photosynthetically active tissues and low or undetectable activity in xylem and root tissues. Fluorometric analysis of GUS activity demonstrated that the larch RbcS promoter was expressed at a 10-fold higher level in leaf blades than in root tissue. Light-grown transgenic plants expressed GUS at a two-fold higher level than dark-grown individuals. These results suggest evolutionary conservation of tissue-specific RbcS promoter activity between gymnosperms and angiosperms but only weak conservation of the transduction mechanism for light regulation.

Chloroplast-dependent and light-independent expression of the tobacco rbcS promoter–GUS chimeric gene in black spruce

1996 ◽  
Vol 26 (6) ◽  
pp. 909-917
Author(s):  
Madoka Gray-Mitsumune ◽  
Bong Y. Yoo ◽  
Pierre J. Charest
Keyword(s):  
Fusion Gene ◽  
Black Spruce ◽  
Gus Activity ◽  
Chimeric Gene ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Zygotic Embryos ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Rbcs Promoter

The tobacco rbcS (ribulose bisphosphate carboxylase small subunit) promoter, fused to the β-glucuronidase (GUS) reporter gene, was delivered to black spruce (Piceamariana (Mill.) BSP) tissues via microprojectile DNA bombardment, and its regulation was studied. The expression of the tobacco rbcS promoter–GUS chimeric gene was dependent on the presence of chloroplasts in black spruce tissues, as demonstrated in two ways: (i) there was no GUS activity expressed in zygotic embryos where no chloroplasts were observed, whereas it was expressed in light- and dark-grown seedlings that contained mature or immature chloroplasts; (ii) a herbicide, Norflurazon, destroyed chloroplast structure in seedlings and inhibited the expression of the tobacco rbcS promoter–GUS chimeric gene. A control chimeric gene, the cauliflower mosaic virus (CaMV) 35S promoter–GUS fusion gene was not inhibited by Norflurazon. Unlike in angiosperms, light had no effect on the expression of tobacco rbcS promoter–GUS chimeric gene. Both light- and dark-grown seedlings showed GUS activity, and expression in dark-grown seedlings was not enhanced by light. These results suggest that the tissue-specific regulation of the rbcS promoter may be conserved between angiosperms and conifers, but that the light regulation of this promoter may not be conserved.

scholarly journals miRNA analysis with Prost! reveals evolutionary conservation of organ-enriched expression and post-transcriptional modifications in three-spined stickleback and zebrafish

2018 ◽  
Author(s):  
Thomas Desvignes ◽  
Peter Batzel ◽  
Jason Sydes ◽  
B. Frank Eames ◽  
John Postlethwait
Keyword(s):  
Gasterosteus Aculeatus ◽  
Mature Mirnas ◽  
Evolutionary Conservation ◽  
Small Rna Sequencing ◽  
Sequencing Data ◽  
Specific Expression ◽  
Tissue Specific ◽  
Mirna Genes ◽  
Evolutionarily Conserved ◽  
Organ Specific

AbstractMicroRNAs (miRNAs) can have tissue-specific expression and functions; they can originate from dedicated miRNA genes, from non-canonical miRNA genes, or from mirror-miRNA genes and can also experience post-transcriptional variations. It remains unclear, however, which mechanisms of miRNA production or modification are tissue-specific and the extent of their evolutionary conservation. To address these issues, we developed the software Prost! (PRocessing Of Short Transcripts), which, among other features, allows accurate quantification of mature miRNAs, takes into account post-transcriptional processing, such as nucleotide editing, and helps identify mirror-miRNAs. Here, we applied Prost! to annotate and analyze miRNAs in three-spined stickleback (Gasterosteus aculeatus), a model fish for evolutionary biology reported to have a miRNome larger than most teleost fish. Zebrafish (Danio rerio), a distantly related teleost with a well-known miRNome, served as comparator. Despite reports suggesting that stickleback had a large miRNome, results showed that stickleback has 277 evolutionary-conserved mir genes and 366 unique mature miRNAs (excluding mir430 gene replicates and the vaultRNA-derived mir733), similar to zebrafish. In addition, small RNA sequencing data from brain, heart, testis, and ovary in both stickleback and zebrafish identified suites of mature miRNAs that display organ-specific enrichment, which is, for many miRNAs, evolutionarily-conserved. These data also supported the hypothesis that evolutionarily-conserved, organ-specific mechanisms regulate miRNA post-transcriptional variations. In both stickleback and zebrafish, miR2188-5p was edited frequently with similar nucleotide editing patterns in the seed sequence in various tissues, and the editing rate was organ-specific with higher editing in the brain. In summary, Prost! is a critical new tool to identify and understand small RNAs and can help clarify a species’ miRNA biology, as shown here for an important fish model for the evolution of developmental mechanisms, and can provide insight into organ-specific expression and evolutionary-conserved miRNA post-transcriptional mechanisms.

scholarly journals Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 235-240
Author(s):  
Y. Wang
Keyword(s):  
Transgenic Tobacco ◽  
Lemna Gibba ◽  
Gus Gene ◽  
Specific Expression ◽  
Bisphosphate Carboxylase ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Rbcs Promoter ◽  
Green Tissue ◽  
Promoter Deletion Analysis

Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteristics of a new L.&nbsp;gibba&nbsp;rbcS promoter driving the expression of the gus gene in transgenic tobacco. A 1491 bp rbcS (small subunit of ribulose bisphosphate carboxylase) promoter was isolated from Lemna gibba. The sequence analysis revealed that this promoter is different from the previously reported rbcS promoter and is named SSU5C. A 1438 bp fragment of the SSU5C promoter was fused with the gus gene and transgenic tobacco plants were generated. The analysis of T<sub>1</sub> tobacco p1438-gus revealed that GUS expression driven by the SSU5C promoter was detected in the green part of vegetative organs. The promoter deletion analysis confirmed a region from position &ndash;152 to &ndash;49 relative to the start of transcription containing boxes X, Y and Z, while a positive regulatory region conferred green tissue-specific expression. Further functional analysis of constructs of box-X, Y, Z, which was fused with the basal SSU5C promoter, confirmed that the boxes X, Y and Z represent the new minimized functional promoter, respectively, and are able to direct green tissue-specific expression. This promoter may be used for gene expression in a tissue-specific manner in plant molecular breeding.

Light regulation and differential tissue-specific expression of phototropin homologues from rice (Oryza sativa ssp. indica)

Plant Science ◽  
2007 ◽  
Vol 172 (1) ◽  
pp. 164-171 ◽  
Author(s):  
Mukesh Jain ◽  
Pooja Sharma ◽  
Shashi B. Tyagi ◽  
Akhilesh K. Tyagi ◽  
Jitendra P. Khurana
Keyword(s):  
Oryza Sativa ◽  
Light Regulation ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Differential Tissue

scholarly journals Atlas of Age- and Tissue-specific DNA Methylation during Early Development of Barley (Hordeum vulgare)

2018 ◽  
Author(s):  
Moumouni Konate ◽  
Michael J. Wilkinson ◽  
Banjamin Mayne ◽  
Eileen Scott ◽  
Bettina Berger ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Hordeum Vulgare ◽  
Organ Function ◽  
Tissue Specific ◽  
Organ Differentiation ◽  
Differentiated Cells ◽  
Differentially Methylated Genes ◽  
Organ Specific ◽  
Development And Differentiation ◽  
Methylation Patterns

The barley (Hordeum vulgare) genome comprises over 32,000 genes, with differentiated cells expressing only a subset of genes; the remainder being silent. Mechanisms by which tissue-specific genes are regulated are not entirely understood, although DNA methylation is likely to be involved. DNA methylation patterns are not static during plant development, but it is still unclear whether different organs possess distinct methylation profiles. Methylation-sensitive GBS was used to generate DNA methylation profiles for roots, leaf-blades and leaf-sheaths from five barley varieties, using seedlings at the three-leaf stage. Differentially Methylated Markers (DMMs) were characterised by pairwise comparisons of roots, leaf-blades and leaf-sheaths of three different ages. While very many DMMs were found between roots and leaf parts, only a few existed between leaf-blades and leaf-sheaths, with differences decreasing with leaf rank. Organ-specific DMMs appeared to target mainly repeat regions, implying that organ differentiation partially relies on the spreading of DNA methylation from repeats to promoters of adjacent genes. Furthermore, the biological functions of differentially methylated genes in the different organs correlated with functional specialisation. Our results indicate that different organs do possess diagnostic methylation profiles and suggest that DNA methylation is important for both tissue development and differentiation and organ function.

Nucleotide sequence of the small subunit of ribulose-1,5-bisphosphate carboxylase from the conifer Larix laricina

1990 ◽  
Vol 14 (2) ◽  
pp. 281-284 ◽  
Author(s):  
Keith W. Hutchison ◽  
Peter D. Harvie ◽  
Patricia B. Singer ◽  
Alan F. Brunner ◽  
Michael S. Greenwood
Keyword(s):  
Nucleotide Sequence ◽  
Small Subunit ◽  
Larix Laricina ◽  
Bisphosphate Carboxylase

scholarly journals Arabidopsis SAURs are critical for differential light regulation of the development of various organs

2016 ◽  
Vol 113 (21) ◽  
pp. 6071-6076 ◽  
Author(s):  
Ning Sun ◽  
Jiajun Wang ◽  
Zhaoxu Gao ◽  
Jie Dong ◽  
Hang He ◽  
...  
Keyword(s):  
Phosphatase Activity ◽  
Protein Phosphatases ◽  
Light Regulation ◽  
Differential Regulation ◽  
Cell Enlargement ◽  
Differential Growth ◽  
Genes Encoding ◽  
Organ Specific ◽  
Cotyledon Expansion ◽  
Mutation Analyses

During deetiolation of Arabidopsis seedlings, light promotes the expansion of cotyledons but inhibits the elongation of hypocotyls. The mechanism of this differential regulation of cell enlargement is unclear. Our organ-specific transcriptomic analysis identified 32 Small Auxin Up RNA (SAUR) genes whose transcripts were light-induced in cotyledons and/or repressed in hypocotyls. We therefore named these SAURs as lirSAURs. Both overexpression and mutation analyses demonstrated that lirSAURs could promote cotyledon expansion and opening and enhance hypocotyl elongation, possibly by inhibiting phosphatase activity of D-clade type 2C protein phosphatases (PP2C-Ds). Light reduced auxin levels to down-regulate the expression of lirSAURs in hypocotyls. Further, phytochrome-interacting factors (PIFs) were shown to directly bind the genes encoding these SAURs and differentially regulate their expression in cotyledons and hypocotyls. Together, our study demonstrates that light mediates auxin levels and PIF stability to differentially regulate the expression of lirSAURs in cotyledons and hypocotyls, and these lirSAURs further mediate the differential growth of these two organs.

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