Analysis of intraspecific nuclear DNA content variation in Gleditsiatriacanthos by flow cytometry

Honeylocust trees (Gleditsiatriacanthos L.) are distributed across a wide range of climatic and edaphic conditions, demonstrating considerable variation in cold, drought, and salinity tolerances. In many angiosperm species, an evolutionary change in nuclear DNA content accompanies colonization into more stressful environments. Fresh leaf nuclei were isolated from 22 honeylocust sources from across the species' native range, mixed with trout red blood cells, stained with propidium iodide, and their DNA content was measured by flow cytometry. According to DNA stainability of the trout red blood cells, leaf nuclei had a DNA content of 1.72 ± 0.02 pg (mean ± SD). Variation in DNA content among the sources was not significant (p < 0.05), suggesting that adaptive evolutionary changes in honeylocust are not evident in increasing or decreasing amounts of leaf nuclear DNA content.

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Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry

Bulletin of Entomological Research ◽

10.1079/ber2005361 ◽

2005 ◽

Vol 95 (4) ◽

pp. 309-312 ◽

Cited By ~ 22

Author(s):

J.K. Brown ◽

G.M. Lambert ◽

M. Ghanim ◽

H. Czosnek ◽

D.W. Galbraith

Keyword(s):

Flow Cytometry ◽

Genome Size ◽

Bemisia Tabaci ◽

Dna Content ◽

Blood Cells ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Chromosome Counting ◽

Male And Female ◽

Haploid Genome Size

AbstractThe nuclear DNA content of the whitefly Bemisia tabaci (Gennnadius) was estimated using flow cytometry. Male and female nuclei were stained with propidium iodide and their DNA content was estimated using chicken red blood cells and Arabidopsis thaliana L. (Brassicaceae) as external standards. The estimated nuclear DNA content of male and female B. tabaci was 1.04 and 2.06 pg, respectively. These results corroborated previous reports based on chromosome counting, which showed that B. tabaci males are haploid and females are diploid. Conversion between DNA content and genome size (1 pg DNA = 980 Mbp) indicate that the haploid genome size of B. tabaci is 1020 Mbp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster Meigen. These results provide an important baseline that will facilitate genomics-based research for the B. tabaci complex.

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Nuclear DNA content variation of herbaceous angiosperm species on 10 global latitudinal transects1,2

The Journal of the Torrey Botanical Society ◽

10.3159/torrey-d-16-00062.1 ◽

2018 ◽

Vol 145 (4) ◽

pp. 340

Author(s):

Jing Yu ◽

Dandan Li ◽

Yuxia Lou ◽

Shuiliang Guo

Keyword(s):

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Content Variation ◽

Dna Content Variation ◽

Angiosperm Species

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Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry

Jurnal Akuakultur Indonesia ◽

10.19027/jai.19.2.142-152 ◽

2020 ◽

Vol 19 (2) ◽

pp. 142-152

Author(s):

Imron ◽

Evi Tahapari ◽

Jadmiko Darmawan ◽

Muhammad Luthfi Abdurachman

Keyword(s):

Flow Cytometry ◽

Phylogenetic Relationship ◽

Dna Content ◽

Interspecific Hybrid ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Pure Line ◽

Dapi Staining ◽

Dna Content Variation ◽

Parental Lines

Nuclear DNA content (NDC) of species or population is believed to have been formed naturally by many mechanisms such chromosomal mutation, insertion and deletion, transposable element, and duplication. Additionally, hybridizations and species’ phylogenetic relationship may also contribute to the NDC diversity. This study was aimed to investigate the profile of NDC in four species Asian catfishes of the genera Pangasius including Pangasionodon hypophthalmus, Pangasius djambal, Pangasius nasusutus, Pangasius nieuwenhuisii, interspecific hybrid of female P. hypophthalmus and male P. djambal (Hybrid HD), and female P. hypophthalmus and male P. nasutus (Hybrid HN). Red blood cells (RBC) were taken from the respective species/groups and NDC measurement was performed in an Attune acoustic flowcytometer (ABI) using DAPI staining and chicken, Gallus domesticus, RBC was used as size reference. The results showed that the mean NDC of P. hypophthalmus, P. djambal. P. nasusutus, P. nieuwenhuisii, were 0.960±0.0254 pg, 1.017±0.0510 pg, 1.000±0.0410 pg, 1.074±0.0231 pg, which are within the range of NDC in the other catfish families The NDC values of Hybrid HD and Hybrid HN were1.005±0.0358 and 0.956± 0.0089, respectively. Among the pure line species, the NDC of P. hypophthalmus was the lowest and was different (P<0.05) from those of P. djambal and P. nieuwenhuisii but was not different (P>0.05) from that of the P. nasutus. The NDC of both Hybrid HD and Hybrid HN were not different form their respective parental lines. However, the NDC profiles of both hybrids were different in that the NDC of the former was in between while the latter was below their respective parental lines. Phylogenetically, the NDC diversity within Pangasiid catfish in this study was independent of their phylogenetic relationship based on cytoplasmic and nuclear markers. Keywords: Flow cytometry, nuclear DNA content, P.hypophthalmus, P. djambal, P. nasutus, P. nieuwenhuisii, interspecific hybrid.

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Nuclear DNA content variation within the genus Daucus (Apiaceae) determined by flow cytometry

Scientia Horticulturae ◽

10.1016/j.scienta.2016.06.023 ◽

2016 ◽

Vol 209 ◽

pp. 132-138 ◽

Cited By ~ 7

Author(s):

Anna Nowicka ◽

Elwira Sliwinska ◽

Dariusz Grzebelus ◽

Rafal Baranski ◽

Philipp W. Simon ◽

...

Keyword(s):

Flow Cytometry ◽

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Content Variation ◽

Dna Content Variation

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Nuclear DNA content in the subgenus Coffea (Rubiaceae): inter- and intra-specific variation in African species

Canadian Journal of Botany ◽

10.1139/b95-002 ◽

1995 ◽

Vol 73 (1) ◽

pp. 14-20 ◽

Cited By ~ 34

Author(s):

J. Cros ◽

M. C. Combes ◽

N. Chabrillange ◽

S. Hamon ◽

C. Duperray ◽

...

Keyword(s):

Flow Cytometry ◽

Dna Content ◽

Interspecific Hybrids ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

African Species ◽

Intermediate Group ◽

Coffea Species ◽

Specific Variation ◽

Angiosperm Species

Flow cytometry has been used to estimate nuclear DNA content of 13 Coffea species (Rubiaceae) native to Africa. Twelve diploid (2n = 22) and one tetraploid (C. arabica, 2n = 44) species were investigated. Isolated nuclei from 77 genotypes were stained with propidium iodide (PI; not base specific). Thirty-nine genotypes were stained with 4′,6-diamidino-2-phenylindole (DAPI; AT specific). Nuclear DNA content (2C values), estimated with PI, ranged from 0.95 to 1.78 pg. By aggregative clustering, three groups of accessions with increasing DNA content were identified. Three species, namely C. sessiliflora, C. racemosa, and C. pseudozanguebariae, had a low DNA content (0.90 – 1.30 pg). Three species, namely C. eugenioides, C. stenophylla, and C. sp. F, were exclusively found in the intermediate group (1.31 – 1.60 pg). The remaining species were distributed between the intermediate group and the last group (1.61 – 1.80 pg). The values determined for the Coffea species are compared, inter- and intra-specifically, to those of other angiosperm species. The observed differences are discussed according to the ecogeographic origin of the species, their phenological characteristics, and the fertility of the F1 interspecific hybrids. Key words: Africa, Coffea, flow cytometry, nuclear DNA content, genome evolution.

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