COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: I. OBSERVATIONS ON SYNTHETIC NUTRIENT MIXTURES INCUBATEDWITH TISSUE CULTURES OF NORMAL KIDNEY

1956 ◽  
Vol 34 (1) ◽  
pp. 273-287
Author(s):  
Ernest Kovacs
Keyword(s):  
Acid Metabolism ◽  
Kidney Tissue ◽  
Nucleic Acid Metabolism ◽  
Cell Physiology ◽  
In Vitro Cultivation ◽  
Tissue Cultures ◽  
Main Interest ◽  
Different Types ◽  
And Behavior

Illustrative examples are given of change in turbidity values of complete and incomplete media used for in vitro cultivation of rhesus monkey kidney tissue. Pools of routine material were examined. The main interest was focused on enzymes directly or indirectly connected with nucleic acid metabolism. The presence and behavior of acid and alkaline monoesterases, 5-nucleotidase, simple nucleotidases, two different types of ribonuclease, and two desoxyribonucleases are described and activity of other enzymes occasionally demonstrated. As a working hypothesis, the bearing of these findings on cell physiology and on pathology of poliomyelitis virus infection is discussed.

COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: I. OBSERVATIONS ON SYNTHETIC NUTRIENT MIXTURES INCUBATEDWITH TISSUE CULTURES OF NORMAL KIDNEY

1956 ◽  
Vol 34 (2) ◽  
pp. 273-287 ◽  
Author(s):  
Ernest Kovacs
Keyword(s):  
Acid Metabolism ◽  
Kidney Tissue ◽  
Nucleic Acid Metabolism ◽  
Cell Physiology ◽  
In Vitro Cultivation ◽  
Tissue Cultures ◽  
Main Interest ◽  
Different Types ◽  
And Behavior

Illustrative examples are given of change in turbidity values of complete and incomplete media used for in vitro cultivation of rhesus monkey kidney tissue. Pools of routine material were examined. The main interest was focused on enzymes directly or indirectly connected with nucleic acid metabolism. The presence and behavior of acid and alkaline monoesterases, 5-nucleotidase, simple nucleotidases, two different types of ribonuclease, and two desoxyribonucleases are described and activity of other enzymes occasionally demonstrated. As a working hypothesis, the bearing of these findings on cell physiology and on pathology of poliomyelitis virus infection is discussed.

COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: II. INVESTIGATION OF VARIOUS ENZYME SYSTEMS IN HOMOGENATES OF KIDNEY TISSUE CULTURES OF NORMAL RHESUS MONKEYS

1956 ◽  
Vol 34 (3) ◽  
pp. 600-618 ◽  
Author(s):  
E. Kovacs
Keyword(s):  
Acid Metabolism ◽  
Kidney Tissue ◽  
Nucleic Acid Metabolism ◽  
Enzyme Assays ◽  
Tissue Cultures ◽  
Functional Tests ◽  
Alkaline Phosphatases ◽  
Enzyme Systems ◽  
Tissue Cultivation ◽  
Considerable Loss

Pools of normal tissue cultures were examined for enzymes associated with nucleic acid metabolism. Ribonucleases and desoxyribonucleases, 5-nucleotidases, simple nucleotidases, acid and alkaline phosphatases were studied, and certain others occasionally demonstrated. Characteristic behavior of these enzyme systems during the cultivation procedures, during growth, and during degeneration was described. Quantitative data indicate the presence of significant amounts of enzymes in the supernatant fluid. This accounts for the considerable loss in these specialized constituents during fluid changes. The bearing of these findings on the physiology and pathology of cultivated cells was discussed, as a working hypothesis, with special emphasis on poliomyelitis infection. The use of enzyme assays, as functional tests supplementing morphological methods in tissue cultivation, was recommended.

COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: II. INVESTIGATION OF VARIOUS ENZYME SYSTEMS IN HOMOGENATES OF KIDNEY TISSUE CULTURES OF NORMAL RHESUS MONKEYS

1956 ◽  
Vol 34 (1) ◽  
pp. 600-618 ◽  
Author(s):  
E. Kovacs
Keyword(s):  
Acid Metabolism ◽  
Kidney Tissue ◽  
Nucleic Acid Metabolism ◽  
Enzyme Assays ◽  
Tissue Cultures ◽  
Functional Tests ◽  
Alkaline Phosphatases ◽  
Enzyme Systems ◽  
Tissue Cultivation ◽  
Considerable Loss

Pools of normal tissue cultures were examined for enzymes associated with nucleic acid metabolism. Ribonucleases and desoxyribonucleases, 5-nucleotidases, simple nucleotidases, acid and alkaline phosphatases were studied, and certain others occasionally demonstrated. Characteristic behavior of these enzyme systems during the cultivation procedures, during growth, and during degeneration was described. Quantitative data indicate the presence of significant amounts of enzymes in the supernatant fluid. This accounts for the considerable loss in these specialized constituents during fluid changes. The bearing of these findings on the physiology and pathology of cultivated cells was discussed, as a working hypothesis, with special emphasis on poliomyelitis infection. The use of enzyme assays, as functional tests supplementing morphological methods in tissue cultivation, was recommended.

scholarly journals Alterations in Protein and Nucleic Acid Metabolism of Thymocytes Produced by Adrenal Steroids in Vitro

1966 ◽  
Vol 241 (7) ◽  
pp. 1646-1648
Author(s):  
Maynard H. Makman ◽  
B. Dvorkin ◽  
Abraham White
Keyword(s):  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism ◽  
Adrenal Steroids

Studies on kidney tubulogenesis VI. Survival and nucleic acid metabolism of differentiating mouse metanephrogenic mesenchyme in vitro

1965 ◽  
Vol 66 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Tapani Vainio ◽  
John Jainchill ◽  
Keith Clement ◽  
Lauri Saxén
Keyword(s):  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism

Studies on the nucleic acid metabolism of renal tumour cells in vitro

1974 ◽  
Vol 2 (1) ◽  
Author(s):  
H. Takayasu ◽  
Y. Aso ◽  
K. Okada ◽  
Y. Hoshino ◽  
K. Koiso ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Metabolism ◽  
Tumour Cells ◽  
Renal Tumour ◽  
Nucleic Acid Metabolism

Nucleic acid metabolism in rat thymocytes X-irradiated in vitro

1971 ◽  
Vol 65 (2) ◽  
pp. 368-376 ◽  
Author(s):  
J GERACI ◽  
G CHRISTENSEN ◽  
K JACKSON
Keyword(s):  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism

scholarly journals THE EFFECTS OF VARIOUS ANTI-CANCER DRUGS ON NUCLEIC ACID METABOLISM OF HUMAN BLADDER CANCER IN VITRO

1977 ◽  
Vol 68 (12) ◽  
pp. 1214-1217
Author(s):  
Takashi Umeda ◽  
Kenkichi Koiso ◽  
Hisao Takayasu
Keyword(s):  
Bladder Cancer ◽  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism ◽  
Human Bladder Cancer ◽  
Cancer Drugs ◽  
Human Bladder ◽  
Anti Cancer

scholarly journals In vitro formation of adventitious shoots on caulinary tissue of physiologically contrasting Agave angustifolia plants

2018 ◽  
pp. 49 ◽  
Author(s):  
Suzel Rios-Ramirez, Rodriguez-Ortiz ◽  
Judith Ruiz- Luna ◽  
Vicente Arturo Velasco- Velasco
Keyword(s):  
Physiological Condition ◽  
Culture Media ◽  
Adventitious Shoots ◽  
Somatic Tissue ◽  
In Vitro Cultivation ◽  
Stem Tissue ◽  
Stock Plant ◽  
Agave Angustifolia ◽  
Different Types

To micropropagate agave plants, somatic tissue is obtained from selected plants that are conditioned for 2 to 6 months to improve their physiological condition and health before in vitro cultivation. The objective of this study was to evaluate the physiological condition of Agave angustifolia plants in terms of its effect on organogenic response in somatic tissue taken from these plants when they are established in similar culture media. In a nursery, growth of four groups of plants was evaluated when they were subjected to different types of irrigation for seven months: 1) water; 2) NS-50% (fertigation with nutrient solution at 50% strength); 3) NS-75%; and 4) NS-100%. At the end of the period, it was found that supplying nutrients is important for plants to achieve better physiological condition. The unfertilized plants and those that received NS-75% had increases of 3.8 and 7.8 leaves, 6.5 and 12.5 cm in length of the largest leaf, and 1610.3 and 4401.4 cm2 in leaf area. Stem tissue was obtained from these stock plants and cultured for 90 days in in vitro culture, and formation of adventitious shoots was assessed. The results showed that the magnitude of organogenesis in stem tissue for formation of adventitious shoots was positively related to the physiological condition of the stock plant. Explants taken from unfertilized stock plants formed 14.6 total shoots and 3.8 shoots on each explant, while those fertigated at 100% concentration of nutrients formed 32.7 total shoots and 8 shoots on each explant.

Nucleic Acid Metabolism in Megaloblastic Marrows in vitro

Nature ◽  
1957 ◽  
Vol 180 (4593) ◽  
pp. 991-992 ◽  
Author(s):  
L. G. LAJTHA ◽  
T. KUMATORI
Keyword(s):  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism
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