The Effect of Hemicholinium-3 on Choline and Acetylcholine Levels in a Sympathetic Ganglion

1975 ◽  
Vol 53 (3) ◽  
pp. 451-457 ◽  
Author(s):  
J. C. Khatter ◽  
A. J. D. Friesen
Keyword(s):  
Superior Cervical Ganglion ◽  
Sympathetic Ganglion ◽  
Transport Systems ◽  
Cholinergic Nerves ◽  
Choline Transport ◽  
Site Of Action ◽  
Cervical Ganglion ◽  
Preganglionic Stimulation ◽  
Rate Of Decline ◽  
Stimulation Of

Preganglionic stimulation of the cat's superior cervical ganglion in the presence of hemicholinium-3 (HC-3) produced the expected depletion of acetylcholine (ACh) stores, but failed to cause a corresponding reduction in the choline content. These results suggest that either HC-3 possesses an intracellular site of action or that in lower doses it selectively inhibits a specialized choline transport system in cholinergic nerves. At a dose of 2 mg/kg, HC-3 probably blocked ACh synthesis completely in ganglia stimulated at 20 Hz. Under these conditions, there was a rapid depletion of ACh to about 50% of control levels during the first 5 min of stimulation and thereafter the rate of decline in ACh levels proceeded at a much slower pace. Since the 2 mg/kg dose of HC-3 did not raise plasma choline concentrations, it may be assumed that non-specialized choline transport systems in other tissues were not significantly inhibited by this dose of HC-3. However, when the dose of HC-3 was increased to 4 mg/kg, plasma choline levels increased by 58%.

scholarly journals The effect of preganglionic stimulation on the incorporation of l-[U-14C]valine into the protein of the superior cervical ganglion of the guinea pig

1970 ◽  
Vol 118 (5) ◽  
pp. 813-818 ◽  
Author(s):  
P. Banks
Keyword(s):  
Guinea Pig ◽  
Superior Cervical Ganglion ◽  
Superior Cervical Ganglia ◽  
Cervical Ganglion ◽  
Cervical Ganglia ◽  
Preganglionic Stimulation ◽  
Energy Dependent ◽  
Stimulation Of

1. Superior cervical ganglia from the guinea pig carry out an energy-dependent incorporation of l-[14C]valine into protein in vitro. 2. Stimulation of the preganglionic nerve at a physiological frequency for more than a few minutes decreases the ability of the ganglia to incorporate labelled valine into protein.

The Effect of Preganglionic Stimulation on the Acetylcholine and Choline Content of a Sympathetic Ganglion

1971 ◽  
Vol 49 (5) ◽  
pp. 375-381 ◽  
Author(s):  
A. J. D. Friesen ◽  
J. C. Khatter
Keyword(s):  
Sympathetic Ganglion ◽  
Storage Capacity ◽  
Experimental Conditions ◽  
Precise Control ◽  
Physiological Conditions ◽  
Cervical Ganglion ◽  
Rapid Restoration ◽  
Preganglionic Stimulation ◽  
Rebound Increase ◽  
Stimulation Of

Preganglionic stimulation of the cat's superior cervical ganglion at 60/s for 2–8 min reduced the ganglion's acetylcholine (ACh) content by about 30%. With continued stimulation, the ACh stores gradually recovered within 15 min. However, when ganglia were allowed to rest following 4 min of stimulation at 60/s not only was there a rapid restoration of the ACh content, but the ACh levels rose to 130% of control after 10 min of rest. Under either of these experimental conditions the choline content increased transiently only after the ACh stores had returned to control values. The above data suggest that there may be a delay in the onset of maximal rates of ACh resynthesis induced by nerve stimulation and that ACh synthesis continues for several minutes after the cessation of the stimulus. In addition, the results are consistent with the concept that about one-third or more of the total ACh stores of a rested ganglion is in a form that can be readily mobilized for release. The observed rebound increase in the ACh content probably means that the ACh storage capacity is not normally saturable and that under most physiological conditions the ACh levels are maintained within certain limits by a precise control of ACh synthesis.

Effects of Electrical Stimulation of the Superior Cervical Ganglion on Cochlear Blood Flow in Guinea Pig

1993 ◽  
Vol 113 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Tian-Ying Ren ◽  
E. Laurikainen ◽  
W. S. Quirk ◽  
J. M. Miller ◽  
A. L. Nuttall
Keyword(s):  
Blood Flow ◽  
Electrical Stimulation ◽  
Guinea Pig ◽  
Superior Cervical Ganglion ◽  
Cochlear Blood Flow ◽  
Cervical Ganglion ◽  
Stimulation Of

Late Discharges in Dog's Sympathetic Ganglia

1972 ◽  
Vol 50 (3) ◽  
pp. 263-269 ◽  
Author(s):  
S. S. Chen
Keyword(s):  
Superior Cervical Ganglion ◽  
Stimulus Frequency ◽  
Sympathetic Ganglia ◽  
Late Response ◽  
Before And After ◽  
Cervical Ganglion ◽  
High Stimulus ◽  
Time Courses ◽  
Preganglionic Stimulation ◽  
Muscarinic And Nicotinic Receptors

In the dog a preganglionic stimulation at a high stimulus frequency for 10–15 s elicited a two-wave response, early and late responses in the perfused ear (vasoconstriction), as well as early and late contractions in the nictitating membrane. Both the late contraction and the late response could be aborted by cooling the superior cervical ganglion, and restored by rewarming the ganglion. Both were resistant to atropine and hexamethonium combined. Their magnitude depended upon the duration of stimulation and upon the stimulus frequency used. Their time courses were similar both before and after hexamethonium or chilling. Therefore, it is concluded that they are manifestations of late discharges in the superior cervical ganglion, which are independent of both muscarinic and nicotinic receptors in the ganglion. A similar but less prominent phenomenon was demonstrated in the lumbosacral sympathetic ganglion of the dog by studying the responses of the retractor penis muscle and the perfused hind limb to preganglionic stimulation.

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