Efficacy and Safety of Faecal Microbiota Transplantation for Acute Pancreatitis: A Randomised, Controlled Study

Aims: We investigated whether faecal microbiota transplantation (FMT) decreases intra-abdominal pressure (IAP) and improves gastrointestinal (GI) dysfunction and infectious complications in acute pancreatitis (AP).Methods: In this first randomised, single-blind, parallel-group, controlled study, we recruited and enrolled consecutive patients with AP complicated with GI dysfunction. Eligible participants were randomly assigned to receive faecal transplant (n = 30) or normal saline (n = 30) via a nasoduodenal tube once and then again 2 days later. The primary endpoint was the rate of IAP decline; secondary endpoints were GI function, infectious complications, organ failure, hospital stay and mortality. Analyses were based on intention to treat.Results: We enrolled 60 participants and randomly assigned them to the FMT (n = 30) or control (n = 30) group. Baseline characteristics and disease severity were similar for both groups. IAP decreased significantly 1 week after intervention in both groups, with no difference in the IAP decline rate between FMT and Control group [0.1 (−0.6, 0.5) vs. 0.2 (−0.2, 0.6); P = 0.27]. Normal gastrointestinal failure (GIF) scores were achieved in 12 (40%) patients in the FMT group and 14 (47%) in the control group, with no significant difference (P = 0.60). However, D-lactate was significantly elevated in the FMT group compared to the control group, as calculated by the rate of decline [−0.3 (−3.7, 0.8) vs. 0.4 (−1.1, 0.9); P = 0.01]. Infectious complications occurred in 15 (50%) and 16 (53.33%) patients in the FMT and control groups, respectively (P = 0.80). However, interleukin-6 (IL-6) was significantly elevated in the FMT group compared to the control group, as calculated by the rate of decline [0.4 (−3.6, 0.9) vs. 0.8 (−1.7, 1.0); P = 0.03]. One participant experienced transient nausea immediately after FMT, but no serious adverse events were attributed to FMT.Conclusion: FMT had no obvious effect on IAP and infectious complications in AP patients, though GI barrier indictors might be adversely affected. Further multi-centre studies are needed to confirm our findings. The study was registered at https://clinicaltrials.gov (NCT02318134).

Are Older Adults’ Difficulties on Emotion Recognition and Theory of Mind Tasks Due to a Decline in General Cognitive Ability?

Older adults have both worse general cognition and worse social cognition. A frequent suggestion is that worse social cognition is due to worse general cognition. However, previous studies have often provided contradictory evidence. The current study examined this issue with a more extensive battery of tasks for both forms of cognition. We gave 47 young and 40 older adults three tasks to assess general cognition (processing speed, working memory, fluid intelligence) and three tasks to assess their social cognition (emotion and theory-of-mind). Older adults did worse on all tasks and there were correlations between general and social cognition. Although working memory and fluid intelligence were unique predictors of performance on the Emotion Photos task and the Eyes task, Age Group was a unique predictor on all three social cognitiaon tasks. Thus, there were relations between the two forms of cognition but older adults continued to do worse than young adults even after accounting for general cognition. We argue that this pattern of results is due to some overlap in brain areas mediating general and social cognition, but also independence, and with a differential rate of decline in brain areas dedicated to general cognition versus social cognition.

Biochemical and transcriptomic analyses of the symbiotic interaction between Cremastra appendiculata and the mycorrhizal fungus Coprinellus disseminatus

Background Cremastra appendiculata is a rare terrestrial orchid with a high market value as an ornamental and medicinal plant. However, the species depends entirely on fungi for seed germination under natural conditions. In a previous study, we have successfully isolated and identified the mycorrhizal fungus Coprinellus disseminatus which was able to induce the germination of C. appendiculata seeds. We then speculated that C. disseminatus may do so by breaking the testa imposed dormancy of the seeds. In this study, biochemical and transcriptomic analyses were used to characterize the germination of C. appendiculata seeds, collected at different stages of germination, as affected by C. disseminatus. Results The lignocellulose in the seeds coat of C. appendiculata was degraded by the mycorrhizal fungus resulting in facilitated absorption of water. The rate of decline in lignin content was 67 and 73% at 6 and 12 days after sowing, respectively. The water content increased from 13 to 90% during symbiosis. A total of 15,382 genes showing significantly different levels of expression (log2 FPKM≥2.0, Qvalue≤0.05) were successfully identified among all libraries, where the highest number of DEGs was shared between 6 days versus 0 day after symbiotic germination. Gene annotation results suggested that 15 key genes related water-status, such as DHN gene family and Xero 1 were down-regulated. The genes zeaxanthin epoxidase ZEP, 9-cis-epoxycarotenoid dioxygenase NCED3 and β-carotene hydroxylase involved in the biosynthesis of abscisic acid (ABA) were significantly down-regulated in 6 days as compared to 0 day after symbiotic germination. Conclusions This work demonstrates that mycorrhizal fungus C. disseminatus can stimulate C. appendiculata seeds germination through a mechanism of breaking the testa imposed dormancy and inducing water absorption of the embryo.

Patient and Clinical Factors at Admission Affect the Levels of Neutralizing Antibodies Six Months after Recovering from COVID-19

The rate of decline in the levels of neutralizing antibodies (NAbs) greatly varies among patients who recover from Coronavirus disease 2019 (COVID-19). However, little is known about factors associated with this phenomenon. The objective of this study is to investigate early factors at admission that can influence long-term NAb levels in patients who recovered from COVID-19. A total of 306 individuals who recovered from COVID-19 at the Tongji Hospital, Wuhan, China, were included in this study. The patients were classified into two groups with high (NAbhigh, n = 153) and low (NAblow, n = 153) levels of NAb, respectively based on the median NAb levels six months after discharge. The majority (300/306, 98.0%) of the COVID-19 convalescents had detected NAbs. The median NAb concentration was 63.1 (34.7, 108.9) AU/mL. Compared with the NAblow group, a larger proportion of the NAbhigh group received corticosteroids (38.8% vs. 22.4%, p = 0.002) and IVIG therapy (26.5% vs. 16.3%, p = 0.033), and presented with diabetes comorbidity (25.2% vs. 12.2%, p = 0.004); high blood urea (median (IQR): 4.8 (3.7, 6.1) vs. 3.9 (3.5, 5.4) mmol/L; p = 0.017); CRP (31.6 (4.0, 93.7) vs. 16.3 (2.7, 51.4) mg/L; p = 0.027); PCT (0.08 (0.05, 0.17) vs. 0.05 (0.03, 0.09) ng/mL; p = 0.001); SF (838.5 (378.2, 1533.4) vs. 478.5 (222.0, 1133.4) μg/L; p = 0.035); and fibrinogen (5.1 (3.8, 6.4) vs. 4.5 (3.5, 5.7) g/L; p = 0.014) levels, but low SpO2 levels (96.0 (92.0, 98.0) vs. 97.0 (94.0, 98.0)%; p = 0.009). The predictive model based on Gaussian mixture models, displayed an average accuracy of 0.7117 in one of the 8191 formulas, and ROC analysis showed an AUC value of 0.715 (0.657–0.772), and specificity and sensitivity were 72.5% and 67.3%, respectively. In conclusion, we found that several factors at admission can contribute to the high level of NAbs in patients after discharge, and constructed a predictive model for long-term NAb levels, which can provide guidance for clinical treatment and monitoring.

Dynamique D’occupation Du Sol Et Diversité Floristique De La Forêt Classée De La Palé (Côte d’Ivoire)

Les forêts classées du Nord de la Côte d’Ivoire connaissent un phénomène d’anthropisation accrue, notamment à la faveur de la crise sociopolitique de 2002 à 2011. A cela, s’ajoute l’effet des changements climatiques qui ont des conséquences sur la qualité de la biodiversité. Cette étude recherche, à travers l’analyse de paramètres floristiques et de la dynamique d’occupation du sol, des informations scientifiques fiables devant guider les orientations d’aménagement d’une forêt classée située dans la Région de la Bagoué, au Nord-Ouest du pays. L’étude de la dynamique de la couverture végétale de la Forêt classée est réalisée à travers une analyse diachronique des images satellitaires Landsat ETM de janvier 2002 et ETM + de mars 2016. L’appréciation des paramètres floristiques est basée sur l’interprétation des données d’inventaire floristique. Les résultats cartographiques montrent une régression des formations boisées passant de 81,75 % en 2002 à 71,19 % en 2016, soit un taux de régression annuel de 0,7 %. Concernant l’étude floristique, au total 281 espèces végétales, réparties entre 210 genres et rangées dans 71 familles, ont été inventoriées dans la forêt classée. Par cette flore, 18 espèces ont présenté un statut particulier, preuve de la valeur de conservation de cette formation végétale. Ce résultat pourrait servir pour la mise en place d’une forêt classée dans la Région de la Bagoué en Côte d’Ivoire. The classified forests of the north of Ivory Coast are experiencing an increased anthropization phenomenon, in particular thanks to the socio-political crisis from 2002 to 2011. In addition to this is the effect of climate change that has consequences on the quality of biodiversity. This paper focuses on the analysis of floristic parameters, the dynamics of land use, and reliable scientific information to guide the development orientations of a classified forest located in the Bagoué Region in the North-West. The study of the dynamics of the vegetation cover of the classified forest was carried out through a diachronic analysis of the Landsat ETM images of January 2002 and ETM + of March 2016. The appreciation of the floristic parameters is based on the interpretation of the floristic inventory data. The cartographic results show a regression of woodlands from 81.75% in 2002 to 71.19% in 2016, i.e., an annual rate of decline of 0.7%. Concerning the floristic study, a total of 281 plant species, divided between 210 genera and arranged in 71 families, were inventoried in the classified forest. Through this flora, 18 species presented a special status, which is a proof of the conservation value of this plant formation. This result could be used for the establishment of a classified forest in Bagoué Region of Côte d’Ivoire.

An Examination of Racial Disparities in Misdemeanor Marijuana Possession Arrests Following Reforms in Four U.S. Jurisdictions

Using data from New York, NY; Los Angeles, CA; Prince George’s County, MD; and Louisville, KY, we examine trends in racial disparities in the enforcement of misdemeanor marijuana possessionbefore and after marijuana reforms. In these jurisdictions, we find that changes to marijuana enforcement were associated with reductions in arrest rates for Black, Hispanic, and White people, though the rate of decline varied by jurisdiction. Black people were arrested at the highest rates in relation to their proportion of the population. In three of the four jurisdictions where issuing criminal citations was an enforcement option, racial/ethnic disparities in arrest rates increased post-reforms; legalization and the option to issue a civil citation were associated with reductions in racial/ethnic disparities. Trends in this study provide policymakers with information to implement effective reforms that target racial disparities in marijuana possession arrests.

The Impact of Local Anti-Smog Resolution in Cracow (Poland) on the Concentrations of PM10 and BaP Based on the Results of Measurements of the State Environmental Monitoring

As a result of conducted air quality policy, including recent legal regulations (the local anti-smog resolution), the number of individual solid fuel heating devices in Cracow (Poland) gradually decreased. Reports on air quality in the city indicate that the concentration of pollutants in Cracow’s air shows a downward trend. However, a similar tendency in terms of improving air quality is also observed in the entire voivodeship, where, as a result of analogous although less radical measures, the number of individual solid fuel heating devices is also decreasing. The paper discusses the impact of legal regulations in Cracow on the improvement of air quality in the context of changes taking place in nearby cities. Trends in changes in PM10 and BaP (PM10) concentrations are analyzed. The rate of decline of the analyzed pollutants concentrations is estimated with the use of nonparametric linear regression. Analysis showed that the rate of decline in the average annual concentrations of PM10 and BaP (PM10) in Cracow is always higher than for the analyzed cities of the Malopolskie Voivodeship. The difference is more pronounced with regard to the months of the heating season. The rate of changes for the average annual BaP (PM10) concentrations in Cracow, compared to other analyzed cities of the Malopolskie Voivodeship, is more intensive than in the case of PM10 concentrations (1.5 times stronger with regard to the months of the heating season). Since the concentration of BaP (PM10) is a better indicator of the effects of liquidation of high-emission furnaces than the concentration of PM10, it can be concluded that the impact of actions related to the improvement of air quality in Cracow in the context of changes taking place in selected cities of the Malopolskie Voivodeship is more visible.

Epidemiological trends in tuberculosis in Ukraine at the beginning of the COVID­-19 pandemic

Objective — to examine the tendencies of the epidemiological situation on tuberculosis in Ukraine at the beginning of the COVID-19 pandemic in 2020. Materials and methods. The analysis of the data of official statistics in 2015—2020, a survey of 157 medical experts and a telephone questionnaire survey of 69 residents of the Volyn region. Results and discussion. It was established that the disease of COVID-19 has found its imprint on the epidemiological situation of tuberculosis and on the functioning of the TB care service. In 2020, the rate of decline in the incidence of pulmonary and extrapulmonary tuberculosis was significantly more intensive than in previous years. However, the incidence of tuberculosis among contacts with bacterial excretion in tuberculosis foci increased 1.85 times in each year. The frequency of tuberculosis recurrences is steadily decreasing.The prevalence and mortality from all forms of active tuberculosis among the entire population of Ukraine has a tendency similar to a decrease in morbidity.The effectiveness of treatment of patients of all categories is proportional to the severity of the tuberculosis process, but still insufficient. Mortality in TB care institutions increased in the previous year against the decrease of this indicator in previous years. Against the background of the inaccessibility of the population to TB care, day hospitals are underused, and the sanatorium network needs to be reorganized. The surgical method of treatment of patients is extremely insufficiently realized. Conclusions. The dispensary contingent of TB care institutions is decreasing every year. There is a reduction in the infrastructure of the TB care service, and in each year, the largest reduction was in the bed stock of TB care institutions and sanatoriums, as well as a decrease in the number of TB doctors.

A Survey of Novae in M83

The results of the first synoptic survey of novae in the barred spiral and starburst galaxy, M83 (NGC 5236), are presented. A total of 19 novae and one background supernova were discovered during the course of a nearly 7 year survey comprised of over 200 individual nights of observation between 2012 December 12 and 2019 March 14. After correcting for the limiting magnitude and the spatial and temporal coverage of the survey, the nova rate in M83 was found to be R = 19+5 −3 yr−1. This rate, when normalized to the K-band luminosity of the galaxy, yields a luminosity-specific nova rate, ν K = 3.0+0.9 −0.6 × 10−10 yr −1 L ⊙,K −1. The spatial distribution of the novae is found to be more extended than the overall galaxy light suggesting that the observed novae are likely dominated by a disk population. This result is consistent with the observed novae light curves, which reveals that the M83 novae are on average more luminous at maximum light and fade faster when compared with novae observed in M31. Generally, the more luminous M83 novae were observed to fade more rapidly, with the complete sample being broadly consistent with a linear maximum magnitude versus rate of decline relation.