Serologic characterization and identification of four lepidopteran cell lines

1976 ◽  
Vol 54 (9) ◽  
pp. 1559-1564 ◽  
Author(s):  
J. Krywienczyk ◽  
S. S. Sohi
Keyword(s):  
Cell Lines ◽  
Tissue Specificity ◽  
Choristoneura Fumiferana ◽  
Double Diffusion ◽  
Malacosoma Disstria ◽  
Tissue Cultures ◽  
Species Identity ◽  
Strong Reaction ◽  
Lepidopteran Cell Lines

The identity of four cell lines (Cf124, Md63, Md108, and Md109) was investigated using the technique of double diffusion. The absorbed antiserum of Cf124 cells formed strong precipitin lines with Choristoneura fumiferana antigen, and the absorbed antisera of Md63, Md108, and Md109 cells gave a strong reaction with the Malacosoma disstria antigen, thus substantiating their species identity. Although the M. disstria cell lines did not show any tissue specificity, they differed from each other in at least one antigen. The antigens from whole larvae of C. fumiferana and M. disstria were more suitable than their hemolymph for serological reactions with the antisera of tissue cultures by double diffusion.

Isozyme characterization of 28 cell lines from five insect species

1985 ◽  
Vol 63 (10) ◽  
pp. 2270-2276 ◽  
Author(s):  
G. T. Harvey ◽  
S. S. Sohi
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Choristoneura Fumiferana ◽  
Insect Species ◽  
Supporting Evidence ◽  
Starch Gel Electrophoresis ◽  
Species Identity ◽  
Starch Gel ◽  
Lepidopteran Cell Lines

Correct identity of cell lines is essential for their use in any investigation; isozyme patterns of cell cultures can give reliable identification. Starch gel electrophoresis was used to develop isozyme profiles of 8 hymenopteran and 20 lepidopteran cell lines and of the insect species from which they were developed. Species identity of 26 of the cell lines was confirmed. For nine of the cell lines these results support the identity established by serological and chromosomal analyses. For the remaining cell lines they provide the first confirmation of species identity. Isozyme profiles of several cell lines from the same species showed unique characteristics that will be useful in monitoring their identity. Two cell lines (IPRI-OL-7 and IPRI-OL-11) considered to be from Orgyia leucostigma appear to contain isozymes of Choristoneura fumiferana. Other supporting evidence and possible causes of this contamination are discussed. These results demonstrate the usefulness of isozyme profiles for the identification and monitoring of cell cultures.

CHROMOSOMAL CHARACTERISATION OF FIVE LEPIDOPTERAN CELL LINES OF MALACOSOMA DISSTRIA (LASIOCAMPIDAE) AND CHRISTONEURA FUMIFERANA (TORTRICIDAE)

1976 ◽  
Vol 18 (3) ◽  
pp. 471-477 ◽  
Author(s):  
T. J. Ennis ◽  
S. S. Sohi
Keyword(s):  
Chromosome Number ◽  
Cell Lines ◽  
Choristoneura Fumiferana ◽  
Malacosoma Disstria ◽  
Large Chromosome ◽  
Cell Nuclei ◽  
Sex Chromatin ◽  
Established Cell ◽  
High Level ◽  
Lepidopteran Cell Lines

Chromosome number and morphology have been examined in four established cell lines (Md63, Md66, Md108, and Md109) of the forest tent caterpillar, Malacosoma disstria Hübner, and one (Cf124) of the spruce budworm, Choristoneura fumiferana (Clemens). Chromosome number distributions of Md63 (mode = 112), Md108 (mode = 103), Md109 (mode = 103), and Cf124 (mode = 110) overlap sufficiently to prevent identification of individual lines by number alone. However, Md66 is exceptional in possessing a modal number of 157. One large chromosome occurs in cells of all lines. The presence of this chromosome, the lack of any distinct polyploid series among chromosome numbers encountered, and the general inverse relationship between number and size of chromosomes, suggest that the high level of heteroploidy characteristic of these and other lepidopteran cell lines reflects not only a possible polyploid origin but also extensive chromosomal rearrangement and fragmentation. Tolerance for such change is attributed to the holokinetic organisation of lepidopteran chromosomes. A distinct heteropycnotic body is present in about 10% of Cf124 cell nuclei, and can be used as a marker for this line. This body may represent the sex chromatin normally encountered in somatic cells of female C. fumiferana.

Comparison of Choriocarcinoma and Normal Placenta

1971 ◽  
Vol 29 ◽  
pp. 324-325
Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord
Keyword(s):  
Cell Lines ◽  
Small Cells ◽  
Tissue Cultures ◽  
Glycogen Depletion ◽  
Cell Surfaces ◽  
Intercellular Spaces ◽  
Concomitant Increase ◽  
Gestational Choriocarcinoma ◽  
Cytoplasmic Glycogen ◽  
Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

Tissue Specificity of Interferon Prepared in Various Tissue Cultures

1962 ◽  
Vol 110 (2) ◽  
pp. 232-234 ◽  
Author(s):  
R. Pollikoff ◽  
M. A. Donikian ◽  
A. Padron ◽  
O. C. Liu
Keyword(s):  
Tissue Specificity ◽  
Tissue Cultures

Lymphoid and other tissue-specific phenotypes of polyomavirus enhancer recombinants: positive and negative combinational effects on enhancer specificity and activity

1986 ◽  
Vol 6 (6) ◽  
pp. 2068-2079
Author(s):  
B A Campbell ◽  
L P Villarreal
Keyword(s):  
Cell Lines ◽  
Heavy Chain ◽  
Murine Leukemia Virus ◽  
Tissue Specificity ◽  
Leukemia Virus ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Lymphoid Cells ◽  
B Lymphoid ◽  
Murine Leukemia

Heterologous enhancer recombinants and deletions of the polyomavirus (Py) noncoding region were constructed and analyzed for tissue specificity of DNA replication and transcription in a number of lymphoid and other cell lines. The simian virus 40 72-base-pair repeat, mouse immunoglobulin heavy-chain enhancer, and Moloney murine leukemia virus enhancer were inserted into the PvuII-D locus (nucleotides 5128 through 5265) of Py. The ability of these recombinants and the parental PvuII-D deletion mutant to replicate in permissive 3T6 cells and MOP-6 cells as well as in nonpermissive mouse B lymphoid, T lymphoid, mastocyte, and embryonal carcinoma cells was determined. Wild-type Py DNA was not permissive for replication in most lymphoid cell lines, except one hybridoma line. Simply deleting the Py PvuII-D region, however, gave Py an expanded host range, allowing high-level replication in some T lymphoid and mastocytoma cell lines, indicating that this element can be a tissue-specific negative as well as positive element. Substitution of the murine leukemia virus enhancer for Py PvuII-D yielded a Py genome which retained the ability to replicate in 3T6 cells but also replicated well in B lymphoid cells. Substitution with the immunoglobulin heavy-chain enhancer allowed replication in B lymphoid cells but interfered with replication in 3T6 cells and mastocytomas. Surprisingly, substitution with the simian virus 40 72-base-pair enhancer repeat gave a recombinant which would not replicate in any cell line tried, including MOP-6 cells, even though other recombinants with this enhancer would replicate. Thus, we observed both cooperation and interference in these combinations between enhancer components and the Py genome and that these combined activities were cell specific. These results are presented as evidence that there may be a positional dependence, or syntax, for the recognition of genetic elements controlling Py tissue specificity.

INFECTION AND MORTALITY OF SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA, AND FOREST TENT CATERPILLAR, MALACOSOMA DISSTRIA, CAUSED BY NUCLEAR AND CYTOPLASMIC POLYHEDROSIS VIRUSES

1969 ◽  
Vol 101 (12) ◽  
pp. 1269-1285 ◽  
Author(s):  
F. T. Bird
Keyword(s):  
Larval Development ◽  
Choristoneura Fumiferana ◽  
Spruce Budworm ◽  
Malacosoma Disstria ◽  
Forest Tent Caterpillar ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Tent Caterpillar

AbstractCytoplasmic polyhedrosis viruses are, in general, more infectious to spruce budworm, Choristoneura fumiferana (Clemens), and forest tent caterpillar, Malacosoma disstria (Hübner), than the nuclear polyhedrosis viruses which affect these insects. The cytoplasmic polyhedrosis viruses interfere with and retard development of the nuclear polyhedrosis viruses.Larvae of both insects, as they grow older, develop resistance to both viruses. Resistance develops more rapidly and to a greater degree against the nuclear polyhedrosis than against the cytoplasmic polyhedrosis viruses.The nuclear polyhedrosis viruses are more lethal than the cytoplasmic polyhedrosis viruses, and all larvae infected with the nuclear polyhedrosis viruses die except those infected so late in larval development that they are able to pupate. Most young larvae infected with the cytoplasmic polyhedrosis virus die or are seriously affected, but infection has progressively less effect as the larvae mature.

Replication of an entomopoxvirus in two lepidopteran cell lines

1990 ◽  
Vol 56 (2) ◽  
pp. 222-232 ◽  
Author(s):  
Tosihiko Hukuhara ◽  
Jinhua Xu ◽  
Kazuhiko Yano
Keyword(s):  
Cell Lines ◽  
Lepidopteran Cell Lines
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