Enzyme electrophoresis, 18S rRNA sequences, and levels of phylogenetic resolution among several species of freshwater planarians (Platyhelminthes, Tricladida, Paludicola)

1992 ◽  
Vol 70 (7) ◽  
pp. 1425-1439 ◽  
Author(s):  
M. Riutort ◽  
K. G. Field ◽  
J. M. Turbeville ◽  
R. A. Raff ◽  
J. Baguña
Keyword(s):  
18S Rrna ◽  
Taxonomic Status ◽  
Sister Group ◽  
Genetic Distances ◽  
Enzyme Polymorphism ◽  
The Other ◽  
Enzyme Electrophoresis ◽  
The Family ◽  
Phylogenetic Resolution ◽  
Rrna Sequences

Enzyme polymorphism and 18S rRNA sequences have been used to measure genetic distances between several species of Platyhelminthes belonging to different taxa including freshwater and parasitic forms. We have used these data to address unresolved phylogenetic and taxonomic problems with this group at several different levels ranging from phylum to subgenus. The main conclusions supported by the data seem to be the following: (i) 18S rRNA data strongly suggest that the Platyhelminthes are monophyletic, being a sister-group to the other Eubilateria; a similar conclusion applies to the Paludicola as to the rest of Platyhelminthes studied; (ii) 18S rRNA and enzyme data indicate that the family Dugesiidae of the Paludicola is monophyletic with respect to the other two families, Planariidae and Dendrocoelidae; and (iii) the subgenus Schmidtea of the genus Dugesia is monophyletic with respect to the other two subgenera of Dugesia, Dugesia and Girardia. Other aspects of the relationships of subgenera and families could not be satisfactorily resolved, but point to new problems that should be addressed in future studies, namely the taxonomic status of the family Planariidae and the relationships between the genera and subgenera of the family Dugesiidae.

Mimaporia, a new genus of Epicopeiidae (Lepidoptera), with description of a new species from Vietnam

Zootaxa ◽  
2017 ◽  
Vol 4254 (5) ◽  
pp. 537 ◽  
Author(s):  
CHIA-HSUAN WEI ◽  
SHEN-HORN YEN
Keyword(s):  
New Species ◽  
New Genus ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Sister Group ◽  
Morphological Evidence ◽  
New Taxon ◽  
The Family ◽  
A New Species

The Epicopeiidae is a small geometroid family distributed in the East Palaearctic and Oriental regions. It exhibits high morphological diversity in body size and wing shape, while their wing patterns involve in various complex mimicry rings. In the present study, we attempted to describe a new genus, and a new species from Vietnam, with comments on two assumed congeneric novel species from China and India. To address its phylogenetic affinity, we reconstructed the phylogeny of the family by using sequence data of COI, EF-1α, and 28S gene regions obtained from seven genera of Epicopeiidae with Pseudobiston pinratanai as the outgroup. We also compared the morphology of the new taxon to other epicopeiid genera to affirm its taxonomic status. The results suggest that the undescribed taxon deserve a new genus, namely Mimaporia gen. n. The species from Vietnam, Mimaporia hmong sp. n., is described as new to science. Under different tree building strategies, the new genus is the sister group of either Chatamla Moore, 1881 or Parabraxas Leech, 1897. The morphological evidence, which was not included in phylogenetic analyses, however, suggests its potential affinity with Burmeia Minet, 2003. This study also provides the first, although preliminary, molecular phylogeny of the family on which the revised systematics and interpretation of character evolution can be based. 

A molecular phylogeny of the circum-Antarctic Opiliones family Neopilionidae

2021 ◽  
Author(s):  
Gonzalo Giribet ◽  
Kate Sheridan ◽  
Caitlin M. Baker ◽  
Christina J. Painting ◽  
Gregory I. Holwell ◽  
...  
Keyword(s):  
New Zealand ◽  
South America ◽  
Morphological Study ◽  
18S Rrna ◽  
New Caledonia ◽  
Sister Group ◽  
28S Rrna ◽  
South American ◽  
Australian Species ◽  
The Family

The Opiliones family Neopilionidae is restricted to the terranes of the former temperate Gondwana: South America, Africa, Australia, New Caledonia and New Zealand. Despite decades of morphological study of this unique fauna, it has been difficult reconciling the classic species of the group (some described over a century ago) with recent cladistic morphological work and previous molecular work. Here we attempted to investigate the pattern and timing of diversification of Neopilionidae by sampling across the distribution range of the family and sequencing three markers commonly used in Sanger-based approaches (18S rRNA, 28S rRNA and cytochrome-c oxidase subunit I). We recovered a well-supported and stable clade including Ballarra (an Australian ballarrine) and the Enantiobuninae from South America, Australia, New Caledonia and New Zealand, but excluding Vibone (a ballarrine from South Africa). We further found a division between West and East Gondwana, with the South American Thrasychirus/Thrasychiroides always being sister group to an Australian–Zealandian (i.e. Australia + New Zealand + New Caledonia) clade. Resolution of the Australian–Zealandian taxa was analysis-dependent, but some analyses found Martensopsalis, from New Caledonia, as the sister group to an Australian–New Zealand clade. Likewise, the species from New Zealand formed a clade in some analyses, but Mangatangi often came out as a separate lineage from the remaining species. However, the Australian taxa never constituted a monophyletic group, with Ballarra always segregating from the remaining Australian species, which in turn constituted 1–3 clades, depending on the analysis. Our results identify several generic inconsistencies, including the possibility of Thrasychiroides nested within Thrasychirus, Forsteropsalis being paraphyletic with respect to Pantopsalis, and multiple lineages of Megalopsalis in Australia. In addition, the New Zealand Megalopsalis need generic reassignment: Megalopsalis triascuta will require its own genus and M. turneri is here transferred to Forsteropsalis, as Forsteropsalis turneri (Marples, 1944), comb. nov.

scholarly journals Debating phylogenetic relationships of the scleractinian Psammocora: molecular and morphological evidences

2007 ◽  
Vol 76 (1) ◽  
pp. 35-54 ◽  
Author(s):  
Francesca Benzoni ◽  
Fabrizio Stefani ◽  
Jaroslaw Stolarski ◽  
Michel Pichon ◽  
Guillaume Mitta ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Phylogenetic Relationships ◽  
Molecular Data ◽  
Genetic Distances ◽  
The Other ◽  
Congeneric Species ◽  
Genetic Affinity ◽  
Phylogenetic Structure ◽  
Skeletal Structures ◽  
The Family

The phylogenetic relationships of the scleractinian genus Psammocora with the other genera traditionally included in the family Siderastreidae and some Fungiidae are assessed based on combined skeletal and molecular data. P. explanulata differs from the other examined congeneric species (P. contigua, P. digitata, P. nierstraszi , P. profundacella, P. superficialis, and P. stellata) in possessing interstomatous septa between adult corallites, costae, and in having continuous buttress-like structures joining septal faces (i.e., fulturae) which typically occur in fungiids. These characters are shared with Coscinaraea wellsi but not with the remainder of the examined siderastreids (the congeneric C. columna, and Anomastraea irregularis, Horastrea indica, Pseudosiderastrea tayamai, Siderastrea savignyana) whose septa are interconnected by typical synapticulae. Most of the examined species form septa with distinct transverse groups of centers of calcification, a biomineralization pattern typical of the Robusta clade. The observations on skeletal structures corroborate the results of the ITS2 and 5.8S molecular phylogeny. C. wellsi and P. explanulata are phylogenetically very close to each other and show closer genetic affinity with the examined Fungiidae (Halomitra pileus, Herpolitha limax, Fungia paumotensis, and Podabacia crustacea) than with the other species in the genera Psammocora and Coscinaraea, or with any other siderastreid. Our results show that neither Psammocora nor Coscinaraea are monophyletic genera. The high genetic distances between the species of Siderastreidae, especially between Pseudosiderastrea tayamai and Siderastrea savignyana on one side and the other genera on the other, suggest a deep divergence in the phylogenetic structure of the family.

Phylogenetic relationships of the family Campulidae (Trematoda) based on 18S rRNA sequences

Parasitology ◽  
1998 ◽  
Vol 117 (4) ◽  
pp. 383-391 ◽  
Author(s):  
M. FERNANDEZ ◽  
D. T. J. LITTLEWOOD ◽  
A. LATORRE ◽  
J. A. RAGA ◽  
D. ROLLINSON
Keyword(s):  
18S Rrna ◽  
Fasciola Hepatica ◽  
18S Rrna Gene ◽  
Host Switching ◽  
Rrna Gene ◽  
Intermediate Hosts ◽  
Marine Gastropods ◽  
Echinostoma Caproni ◽  
The Family ◽  
Rrna Sequences

Traditionally, the family Campulidae has been associated either with the family Fasciolidae, parasites of ruminants, or the Acanthocolpidae, parasites of fishes, based on morphological similarities. Since morphology does not seem to resolve clearly the problem of the relationships of campulids, we have used the sequences of the 18S rRNA gene of the campulids Zalophotrema hepaticum, Campula oblonga and Nasitrema globicephalae, the fasciolid Fasciola hepatica, the acanthocolpid Stephanostomum baccatum and the outgroup Schistosoma mansoni to infer a phylogeny. Maximum parsimony and neighbour-joining methods were applied. Both methods indicated that campulids are closer to acanthocolpids than fasciolids. In order to confirm this relationship, we generated a second phylogeny using all the partial sequences of the 18S published for trematodes: Lobatostoma manteri, Echinostoma caproni, Calicophoron calicophorum, Tetracerasta blepta, Gyliauchen sp. and Opistorchis viverrini, plus those mentioned above, and Dicrocoelium dendriticum. The aspidogastrean L. manteri was used as the outgroup. Results were identical to the first analysis. According to this and the most recent Digenean phylogeny, which considers campulids and acanthocolpids as sister groups, we suggest that a common origin for these 2 groups would imply a host-switching process. The life-cycle of acanthocolpids includes marine gastropods as first intermediate hosts, and fishes as second intermediate and definitive hosts. In this context, the hypothesis would be that trematodes whose cycle ended in fishes were able to switch to mammalian hosts.

scholarly journals Genetic analysis ofClavibacter toxicus, the agent of annual ryegrass toxicity

1996 ◽  
Vol 117 (2) ◽  
pp. 393-400 ◽  
Author(s):  
M. S. Johnston ◽  
S. S. Sutherland ◽  
C. C. Constantine ◽  
D. J. Hampsons
Keyword(s):  
Genetic Distances ◽  
The Other ◽  
Enzyme Electrophoresis ◽  
Annual Ryegrass ◽  
Field Isolates ◽  
Multilocus Enzyme Electrophoresis ◽  
Related Group ◽  
Western Australian ◽  
Reference Strains ◽  
Annual Ryegrass Toxicity

SummaryMultilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates ofClavibacter toxicus, the agent of annual ryegrass toxicity. These included 37 Western Australian (WA) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in Australia over approximately 8 years. Seventeen reference strains for the related generaCurtobacterium, RhodococcusandArthrobacterwere examined for comparison. The 69 isolates were divided into 29 electrophoretic types (ETs), separated by genetic distances of 0·06 to 0·81. TheC. toxicusisolates fell into 12 ETs, 11 of which formed a tightly clustered group separated by a genetic distance of 0·23 or less. Thirty-one of the WA field isolates ofC. toxicusfell into a single ET, and four into another ET.Clavibacter toxicustherefore formed a closely related group which was genetically distinct from the other plant pathogenic species, and a dominant widely disseminated strain of the species was identified in WA.

Genetic evidence supports Sylvilagus mansuetus (Lagomorpha: Leporidae) as a subspecies of S. bachmani

Zootaxa ◽  
2016 ◽  
Vol 4196 (2) ◽  
pp. 289
Author(s):  
SERGIO TICUL ÁLVAREZ-CASTAÑEDA ◽  
CONSUELO LORENZO
Keyword(s):  
Phylogenetic Relationships ◽  
Morphological Traits ◽  
Nuclear Gene ◽  
Sister Group ◽  
Genetic Distances ◽  
Mitochondrial Genes ◽  
The Other ◽  
Cyt B ◽  
San Jose ◽  
Allopatric Distribution

Since Sylvilagus bachmani (Lagomorpha: Leporidae) from the Baja California Peninsula and S. mansuetus from San Jose Island, Mexico, display an allopatric distribution and are closely related, their taxonomy is unclear. The phylogenetic relationships among specimens of both species were evaluated using two mitochondrial genes (Cyt b, COI) and the beta-fibrinogen nuclear gene intron 7 (β-fib I7). The genetic analyses revealed that S. mansuetus was included within the S. bachmani clade as the sister-group of S. b. cerrosensis. The genetic distances among S. b. cerrosensis and mansuetus were relatively low (1.3% with Cyt b), similar to intraspecific distances observed within other species of Sylvilagus. We consider mansuetus to be a subspecies of S. bachmani, and the morphological traits previously used to differentiate the two taxa should be used to distinguish S. b. mansuetus from the other subspecies of S. bachmani. 

Enzyme polymorphism in Schistosoma mattheei from cattle in the Eastern Transvaal Lowveld

1989 ◽  
Vol 63 (3) ◽  
pp. 191-196 ◽  
Author(s):  
F. J. Kruger
Keyword(s):  
Adult Worm ◽  
Malate Dehydrogenase ◽  
Enzyme Polymorphism ◽  
The Other ◽  
Enzyme Electrophoresis ◽  
Weinberg Equilibrium ◽  
Hardy Weinberg Equilibrium ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACTEnzyme electrophoresis was conducted on 10 Schistosoma mattheei adult worm samples, comprising 270 individuals, collected from cattle in the Eastern Transvaal Lowveld. Glucose-6-phosphate dehydrogenase (G6PDH) was studied in all the samples and phosphoglucomutase (PGM) and malate dehydrogenase (MDH) in five populations each. Only one population was polymorphic for G6PDH. In this population, in addition to the allele found in all the other samples, a second allele occurred with a similar Rf value to S. haematobium. The two alleles were in Hardy-Weinberg equilibrium. MDH-1 exhibited two alleles. However, these alleles were not in equilibrium. In certain populations, heterozygotes occurred together with homozygotes of one of the alleles only. PGM was monomorphic in all the populations studied.

The first fossil horntail wasp (Hymenoptera: Siricidae) from Lower Cretaceous Crato Formation in Brazil

2020 ◽  
Vol 3 (4) ◽  
pp. 382-389
Author(s):  
CORENTIN JOUAULT ◽  
JEAN-MARC POUILLON ◽  
ANDRÉ NEL
Keyword(s):  
Lower Cretaceous ◽  
New Genus ◽  
Sister Group ◽  
The Other ◽  
Northeastern Brazil ◽  
Crown Group ◽  
The Family ◽  
Group Relationships ◽  
Fossil Records ◽  
New Discovery

A new wood wasp, Cratosirex sennlaubi gen. et sp. nov., is described and figured from one specimen collected from the Lower Cretaceous Crato Formation in northeastern Brazil. This new genus is placed in the new siricid subfamily Cratosiricinae subfam. nov., based on a combination of plesiomorphic and autapomorphic characters. The presence of small and sub-equal forewing cells 1R1 and 2R1 is a synapomorphy with the extant subfamily Siricinae, absent in the other extinct subfamilies †Auliscinae and †Gigasiricinae, supporting a sister group relationships with the Siricinae. Our new discovery expands the distribution range of Siricidae fossil records, highlights the antiquity of the family, and emphasizes the need for more studies of this particular insect lineage in the Mesozoic deposits. Currently, all the representatives of the crown group of the extant Siricidae are Cenozoic.

A phylogenetic reassessment of the monophyletic status of the family Soleidae, with comments on the suborder Soleoidei (Pisces; Pleuronectiformes)

1988 ◽  
Vol 66 (12) ◽  
pp. 2797-2810 ◽  
Author(s):  
François Chapleau ◽  
Allen Keast
Keyword(s):  
Cladistic Analysis ◽  
Sister Group ◽  
The Other ◽  
Anatomical Data ◽  
The Family ◽  
Family Level

This article presents the phylogenetic conclusions of an osteological study of species belonging to the subfamilies (Soleinae and Achirinae) of the dextral flatfish family Soleidae (Pieuronectiformes; Soleoidei). A cladistic analysis of the data revealed that the subfamilies, but not the family, are monophyletic. The Soleinae were found to be more closely related to the other soleoid family, the Cynoglossidae, than to the Achirinae. If we accept the principle that only monophyletic groups are to be admitted into Linnean classification, it is suggested that the two subfamilies be raised to the family level. The anatomical data led to the proposal (with caution) that the sister group of the achirid–soleid–cynoglossid lineage is the pleuronectid subfamily Samarinae. Consequently, it is suggested that the suborder Soleoidei be eliminated by incorporating its three families into the Pleuronectoidei which becomes monophyletic. Also, it is proposed that the Pleuronectid subfamilies (Pleuronectinae, Poecilopsettinae, Paralichthodinae, Rhombosoleinae, Samarinae) be raised to the family level. However, since the monophyletic status of these taxa is dubious (except for the Samarinae) any hypothesis including them must await a proper cladistic analysis of their intra- and inter-relationships.

scholarly journals Phylogeny of the Anomura (Decapoda, Crustacea): Spermatozoa and spermatophore morphological evidence

1997 ◽  
Vol 67 (2) ◽  
pp. 125-141 ◽  
Author(s):  
Christopher C. Tudge
Keyword(s):  
Phylogenetic Analysis ◽  
Decapod Crustacean ◽  
Sister Group ◽  
Morphological Characters ◽  
The Other ◽  
Monophyletic Group ◽  
Morphological Evidence ◽  
Monophyletic Clade ◽  
The Family ◽  
Single Clade

A phylogenetic analysis of selected anomuran, thalassinidean, and other decapod crustacean taxa, based on spermatozoal ultrastructural characters and spermatophore morphological characters, was performed and the following relationships of the taxa are elucidated from the trees produced. The Anomura are not a monophyletic assemblage, with the lomoid Lomis being exclusive of the remainder of the anomuran taxa, and the thalassinid Thalassina included in the anomuran clade. The synapomorphy joining the majority of the conventional anomuran taxa (Lomis excluded) is the cytoplasmic origin of the microtubular arms. When the palinurid and thalassinoid representatives are separately designated as outgroups, the Astacidea and Brachyura jointly formed a sister group to the Anomura. The superfamilies Thalassinoidea, Paguroidea, and Galatheoidea are not monophyletic groups. In all analyses the anomuran families Coenobitidae and Porcellanidae each form a monophyletic group. The paguroid family Diogenidae is paraphyletic, with the genera Clibanarius and Cancellus separate from a single clade containing the remaining diogenid genera. The families Paguridae and Parapaguridae form a monophyletic clade with the exception of Porcellanopagurus. The two representatives of the family Chirostylidae (Eumunida and Uroptychus) fail to associate with the other species in the Galatheoidea. The taxa in the family Galatheidae are not a monophyletic assemblage. The only investigated hippoid Hippa is portrayed as the sister group to the remainder of the anomuran taxa (with the exception of Lomis).

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