HPLC-MS/MS Measurement of Oxidative Base Damage to Isolated and Cellular DNA

2002 ◽  
pp. 190-202 ◽  
Author(s):  
T. Douki ◽  
J.-L. Ravanat ◽  
S. Frelon ◽  
A.-G. Bourdat ◽  
J.-P. Pouget ◽  
...  
Keyword(s):  
Base Damage ◽  
Cellular Dna ◽  
Oxidative Base Damage

O XVI A.4 Measurement of oxidative base damage within cellular DNA and biological fluids

1997 ◽  
Vol 379 (1) ◽  
pp. S154
Author(s):  
Jean Cadet ◽  
Thierry Douki ◽  
Didier Molko ◽  
Jean-Luc Ravanat ◽  
Sylvie Sauvaigo ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Base Damage ◽  
Cellular Dna ◽  
Oxidative Base Damage

Oxidative base damage to DNA: specificity of base excision repair enzymes

2000 ◽  
Vol 462 (2-3) ◽  
pp. 121-128 ◽  
Author(s):  
Jean Cadet ◽  
Anne-Gaëlle Bourdat ◽  
Cédric D'Ham ◽  
Victor Duarte ◽  
Didier Gasparutto ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Base Damage ◽  
Repair Enzymes ◽  
Base Excision ◽  
Dna Specificity ◽  
Oxidative Base Damage

scholarly journals Oxidative DNA base modifications as factors in carcinogenesis.

1998 ◽  
Vol 45 (2) ◽  
pp. 561-572 ◽  
Author(s):  
R Olinski ◽  
P Jaruga ◽  
T H Zastawny
Keyword(s):  
Metastatic Potential ◽  
Base Damage ◽  
Normal Tissues ◽  
Dna Base ◽  
Dna Modifications ◽  
Cancer Initiation ◽  
Base Modifications ◽  
Therapeutic Doses ◽  
Oxidative Base Damage

Reactive oxygen species can cause extensive DNA modifications including modified bases. Some of the DNA base damage has been found to possess premutagenic properties. Therefore, if not repaired, it can contribute to carcinogenesis. We have found elevated amounts of modified bases in cancerous and precancerous tissues as compared with normal tissues. Most of the agents used in anticancer therapy are paradoxically responsible for induction of secondary malignancies and some of them may generate free radicals. The results of our experiments provide evidence that exposure of cancer patients to therapeutic doses of ionizing radiation and anticancer drugs causes base modifications in genomic DNA of lymphocytes. Some of these base damages could lead to mutagenesis in critical genes and ultimately to secondary cancers such as leukemias. This may point to an important role of oxidative base damage in cancer initiation. Alternatively, the increased level of the modified base products may contribute to genetic instability and metastatic potential of tumor cells.

Facts and artifacts in the measurement of oxidative base damage to DNA

1998 ◽  
Vol 29 (6) ◽  
pp. 541-550 ◽  
Author(s):  
Jean Cadet ◽  
Cedric D'Ham ◽  
Thierry Douki ◽  
Jean-Pierre Pouget ◽  
Jean-Luc Ravanat ◽  
...  
Keyword(s):  
Base Damage ◽  
Oxidative Base Damage

Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA

1993 ◽  
Vol 14 (9) ◽  
pp. 1733-1735 ◽  
Author(s):  
Andrew R. Collins ◽  
Susan J. Duthie ◽  
Victoria L. Dobson
Keyword(s):  
Human Lymphocyte ◽  
Base Damage ◽  
Oxidative Base Damage

Melanin Both Causes and Prevents Oxidative Base Damage in DNA: Quantification by Anti-Thymine Glycol Antibody

1992 ◽  
Vol 130 (2) ◽  
pp. 160 ◽  
Author(s):  
Karen Hubbard-Smith ◽  
Helene Z. Hill ◽  
George J. Hill
Keyword(s):  
Dna Quantification ◽  
Base Damage ◽  
Thymine Glycol ◽  
Oxidative Base Damage

scholarly journals Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage.

1986 ◽  
Vol 6 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
D E Helland ◽  
P W Doetsch ◽  
W A Haseltine
Keyword(s):  
Ionizing Radiation ◽  
Substrate Specificity ◽  
Osmium Tetroxide ◽  
Oxidative Dna Damage ◽  
Uv Light ◽  
Base Damage ◽  
Pyrimidine Base ◽  
Endonuclease Iii ◽  
Calf Thymus ◽  
Oxidative Base Damage

The substrate specificity of a calf thymus endonuclease on DNA damaged by UV ligh, ionizing radiation, and oxidizing agents was investigated. End-labeled DNA fragments of defined sequence were used as substrates, and the enzyme-generated scission products were analyzed by using DNA sequencing methodologies. The enzyme was shown to incise damaged DNA at pyrimidine sites. The enzyme incised DNA damaged with UV light, ionizing radiation, osmium tetroxide, potassium permanganate, and hydrogen peroxide at cytosine and thymine sites. The substrate specificity of the calf thymus endonuclease was compared to that of Escherichia coli endonuclease III. Similar pyrimidine base damage specificities were found for both enzymes. These results define a highly conserved class of enzymes present in both procaryotes and eucaryotes that may mediate an important role in the repair of oxidative DNA damage.

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