DEVELOPMENT OF AN EVANESCENT FIELD SYSTEM FOR REAL-TIME DETECTION OF DNA HYBRIDIZATION

AsiaNano 2002 ◽  
2003 ◽  
Author(s):  
DO-KYUN KIM ◽  
YOUNG-SOO KWON ◽  
EIICHI TAMIYA
2002 ◽  
Vol 01 (05n06) ◽  
pp. 663-666
Author(s):  
DO-KYUN KIM ◽  
YOUNG-SOO KWON ◽  
EIICHI TAMIYA

In this research, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. For detection of DNA hybridization assay, a high-density array of sensor probes were prepared by randomly distributing a mixture of particles immobilized with oligonucleotides for DNA chip applications. With the evanescent field excitation and real-time detection method, we suggest that a very sharp discrimination of bulk fluorescence against surface excitation in combination with high excitation intensities can be achieved.


1995 ◽  
Vol 92 (14) ◽  
pp. 6379-6383 ◽  
Author(s):  
D. I. Stimpson ◽  
J. V. Hoijer ◽  
W. T. Hsieh ◽  
C. Jou ◽  
J. Gordon ◽  
...  

2010 ◽  
Vol 25 (12) ◽  
pp. 2579-2585 ◽  
Author(s):  
Larbi Touahir ◽  
Elisabeth Galopin ◽  
Rabah Boukherroub ◽  
Anne Chantal Gouget-Laemmel ◽  
Jean-Noël Chazalviel ◽  
...  

2005 ◽  
Vol 1 (1) ◽  
pp. 065-070 ◽  
Author(s):  
Kagan Kerman ◽  
Yasutaka Morita ◽  
Yuzuru Takamura ◽  
Eiichi Tamiya ◽  
Kenzo Maehashi ◽  
...  

2014 ◽  
Vol 105 (3) ◽  
pp. 031103 ◽  
Author(s):  
Yong Fan ◽  
Kazuhiro Hotta ◽  
Akira Yamaguchi ◽  
Yu Ding ◽  
Yonghong He ◽  
...  

2011 ◽  
Vol 26 (5) ◽  
pp. 1942-1946 ◽  
Author(s):  
Takeyuki Mogi ◽  
Keiichi Hatakeyama ◽  
Tomoyuki Taguchi ◽  
Hitoshi Wake ◽  
Takeo Tanaami ◽  
...  

1995 ◽  
Vol 67 (23) ◽  
pp. 4283-4289 ◽  
Author(s):  
Helen J. Watts ◽  
Debra. Yeung ◽  
Helen. Parkes

2012 ◽  
Author(s):  
Anthony D. McDonald ◽  
Chris Schwarz ◽  
John D. Lee ◽  
Timothy L. Brown

Sign in / Sign up

Export Citation Format

Share Document