Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides.

In this research, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. For detection of DNA hybridization assay, a high-density array of sensor probes were prepared by randomly distributing a mixture of particles immobilized with oligonucleotides for DNA chip applications. With the evanescent field excitation and real-time detection method, we suggest that a very sharp discrimination of bulk fluorescence against surface excitation in combination with high excitation intensities can be achieved.

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Damage profile and mode observation in LiTaO3 by low-energy H+ ions

Canadian Journal of Physics ◽

10.1139/p01-045 ◽

2001 ◽

Vol 79 (6) ◽

pp. 921-927

Author(s):

K -M Wang ◽

H Hu ◽

F Chen ◽

F Lu ◽

J -H Zhang ◽

...

Keyword(s):

Lower Energy ◽

Rutherford Backscattering ◽

Low Energy ◽

Coupling Method ◽

Optical Wave ◽

Wave Guides ◽

Transport Of Ions ◽

Prism Coupling ◽

Optical Wave Guides

LiTaO3 samples were implanted with 50, 80, 120, and 150 keV H + ions. The damage profiles in LiTaO3 induced by H+ ions were investigated using the Rutherford backscattering/channeling technique. We used TRIM (transport of ions in matter) to simulate it. It was found that the shape of the experimental damage profile was similar to one predicted by TRIM'98, but the experimental damage ratio (NDN) was higher than the calculated damage ratio based on TRIM'98. The modes in LiTaO3 were measured by a prism-coupling method for all samples. The modes were observed only for LiTaO3 implanted with 80, 120, and 150 keV H+ ions. The present result shows that optical wave guides in LiTaO3 can be formed by lower energy H+ ions. PACS Nos.: 61.72-y, 61.82-d, 61.85+p, 78.20Ci

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Localized surface plasmon-enhanced fluorescence spectroscopy for highly-sensitive real-time detection of DNA hybridization

Biosensors and Bioelectronics ◽

10.1016/j.bios.2010.04.026 ◽

2010 ◽

Vol 25 (12) ◽

pp. 2579-2585 ◽

Cited By ~ 50

Author(s):

Larbi Touahir ◽

Elisabeth Galopin ◽

Rabah Boukherroub ◽

Anne Chantal Gouget-Laemmel ◽

Jean-Noël Chazalviel ◽

...

Keyword(s):

Fluorescence Spectroscopy ◽

Real Time ◽

Surface Plasmon ◽

Dna Hybridization ◽

Localized Surface Plasmon ◽

Enhanced Fluorescence ◽

Plasmon Enhanced Fluorescence ◽

Highly Sensitive ◽

Real Time Detection

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Disorder-induced mixing of InGaAs/InP multiple quantum wells by phosphorus implantation for optical wave-guides

Semiconductor Science and Technology ◽

10.1088/0268-1242/5/11/515 ◽

1990 ◽

Vol 5 (11) ◽

pp. 1146-1146

Author(s):

N J Whitehead ◽

W P Gillin ◽

I V Bradley ◽

B L Weiss ◽

P Claxton

Keyword(s):

Quantum Wells ◽

Multiple Quantum Wells ◽

Multiple Quantum ◽

Optical Wave ◽

Wave Guides ◽

Optical Wave Guides

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3-D Woven Composites Instrumented With EFPI Fiber Optic Sensors

Nondestructive Evaluation ◽

10.1115/imece2002-33480 ◽

2002 ◽

Cited By ~ 1

Author(s):

Alexander Bogdanovich ◽

Donald Wigent ◽

Thomas J. Whitney ◽

Paul A. Clark

Keyword(s):

Fiber Optic ◽

Mechanical Damage ◽

Polymeric Composite ◽

Fiber Optic Sensors ◽

Woven Composites ◽

Optical Wave ◽

Strain Monitoring ◽

Novel Approach ◽

Wave Guides ◽

Optical Wave Guides

A novel approach to continuous health monitoring of polymeric composite materials and structural elements using embedded Extrinsic Fabry-Perot Interferometers (EFPI) is proposed and validated. The proof of concept includes several consecutive steps. First, it is verified that simple optical wave guides survived a regular 3-D weaving process. Then EFPI sensor assemblies are manually incorporated into the preforms and it is verified that they are functional. Next step is resin infusion of instrumented preforms using VARTM method, followed by investigation of possible mechanical damage to sensor leads. Finally, test specimens are fabricated, and four-point bending tests are performed. The internal strain monitoring results provided by the embedded fiber optic sensors are compared to the data from surface foil gages. The developed approach validates, particularly, the possibility of continuous through-thickness strain monitoring, which is crucial for composite bonded and bolted joints, components with holes, openings, stiffeners, and other cases of high strain gradients.

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