DEVELOPMENT OF AN EVANESCENT FIELD SYSTEM FOR REAL-TIME DETECTION OF DNA HYBRIDIZATION
2002 ◽
Vol 01
(05n06)
◽
pp. 663-666
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In this research, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. For detection of DNA hybridization assay, a high-density array of sensor probes were prepared by randomly distributing a mixture of particles immobilized with oligonucleotides for DNA chip applications. With the evanescent field excitation and real-time detection method, we suggest that a very sharp discrimination of bulk fluorescence against surface excitation in combination with high excitation intensities can be achieved.
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2019 ◽
Vol 60
◽
pp. 295-304
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