FISH OTOLITHS ANALYSIS BY PIXE: APPLICATION TO STOCK DISCRIMINATION

Fish otoliths are continuously dsposited from fish birth to its death along with encoding environmental information. In order to decode the information, PIXE was adopted as trace elemental analysis of the otoliths. Strontium to calcium concentration ratios of red sea bream otoliths varied among rearing stations. The Sr/Ca ratios of Lake Biwa catfishes also varied between male and female and among fishing grounds. The PIXE analysis was applied to the fish stock discrimination.

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PIXE ANALYSIS OF OTOLITHS FROM REARED RED SEA BREAM, Pagrus major(Temminck et Schlegl)

International Journal of PIXE ◽

10.1142/s0129083593000239 ◽

1993 ◽

Vol 03 (04) ◽

pp. 275-281 ◽

Cited By ~ 4

Author(s):

NOBUAKI ARAI ◽

WATARU SAKAMOTO

Keyword(s):

Red Sea ◽

Calcium Concentration ◽

Seawater Temperature ◽

Marine Fishes ◽

Sea Bream ◽

Red Sea Bream ◽

Pagrus Major ◽

Mineral Concentration ◽

Pixe Analysis ◽

X Ray

PIXE analysis was applied to estimate mineral concentration in red sea bream otoliths without cutting or polishing. Detected elements include Sr, Fe, Mn and Zn, which are commonly found in the otoliths in marine fishes. Strontium-Calcium concentration ratio is calculated by means of combined X-ray yields. The ratio doesn’t indicate dear correlaion with mean reared seawater temperature. It is caused by the diffraction error induced by rough topographies of the otoliths surface.

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PIXE ANALYSIS OF OTOLITHS FROM SEVERAL SPECIES OF TELEOST FISH

International Journal of PIXE ◽

10.1142/s0129083594000167 ◽

1994 ◽

Vol 04 (02n03) ◽

pp. 123-129 ◽

Cited By ~ 2

Author(s):

NOBUAKI ARAI ◽

WATARU SAKAMOTO ◽

KUNIKO MAEDA

Keyword(s):

Trace Elements ◽

Red Sea ◽

Teleost Fish ◽

Seawater Temperature ◽

Trace Element Composition ◽

Element Composition ◽

Sea Bream ◽

Red Sea Bream ◽

Pixe Analysis ◽

Fish Otoliths

In-air PIXE was used to analyze trace elements in otoliths from several species of teleost fish in order to examine the correlation between the trace elements concentrations and environmental conditions. Mn, Fe, Zn and Sr were detected accurately in the order of ppm by using the in-air PIXE. It seemed that concentrations of Sr and Zn in red sea bream otoliths increased in proportion to higher seawater temperature. In addition, there were significant differences in trace element composition between that of reared red sea bream and rockfish and that of wild ones. Preliminary results indicate that the PIXE is a powerful technique to investigate fish otoliths.

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PIXE Analysis of Fish Otoliths: Application to Fish Stock Discrimination

International Journal of PIXE ◽

10.1142/s0129083596000223 ◽

1996 ◽

Vol 06 (01n02) ◽

pp. 227-232 ◽

Cited By ~ 1

Author(s):

NOBUAKI ARAI ◽

WATARU SAKAMOTO ◽

KOJI TATENO ◽

KOJI YOSHIDA

Keyword(s):

Trace Elements ◽

Sea Of Japan ◽

Japanese Flounder ◽

Fish Stock ◽

The Sea Of Japan ◽

Pixe Analysis ◽

Fish Stocks ◽

Stock Discrimination ◽

Main Component ◽

Fish Otoliths

PIXE was adopted to analyze trace elements in otoliths of Japanese flounder to discriminate among several local fish stocks. The otoliths were removed from samples caught at five different sea areas along with the coast of the Sea of Japan: Akita, Ishikawa, Kyoto (2 stations), and Fukuoka. Besides calcium as main component, strontium, manganese, and zinc were detected. Especially Sr concentrations were different among 4 areas except between 2 stations in Kyoto. It suggested that the fish in the 2 stations in Kyoto were the same stock differed to the others.

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Correlation between Ambient Seawater Temperature and Strontium-calcium Concentration Ratios in Otoliths of Red Sea Bream Pagrus Major

Fisheries Science ◽

10.2331/fishsci.62.652 ◽

1996 ◽

Vol 62 (4) ◽

pp. 652-653 ◽

Cited By ~ 12

Author(s):

Nobuaki Arai ◽

Wataru Sakamoto ◽

Kuniko Maeda

Keyword(s):

Red Sea ◽

Calcium Concentration ◽

Seawater Temperature ◽

Sea Bream ◽

Red Sea Bream ◽

Pagrus Major ◽

Ambient Seawater ◽

Concentration Ratios

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MICRO-PIXE ANALYSIS OF FISH OTOLITHS: METHODOLOGY AND EVALUATION OF FIRST RESULTS FOR STOCK DISCRIMINATION

International Journal of PIXE ◽

10.1142/s0129083592000385 ◽

1992 ◽

Vol 02 (03) ◽

pp. 357-379 ◽

Cited By ~ 35

Author(s):

SOEY H. SIE ◽

RONALD E. THRESHER

Keyword(s):

Trace Elements ◽

High Sensitivity ◽

Element Distribution ◽

Trace Element Distribution ◽

Pixe Analysis ◽

Stock Discrimination ◽

First Results ◽

Sample Preparation Procedure ◽

Fish Otoliths ◽

Significant Data

Micro-PIXE has been used to measure the trace element distribution in otoliths from several species of ocean fish, in order to investigate its possible use in stock discrimination. Trace elements detected include Sr, Fe, Mn, Ni, Zn, Cu, Se, Cd, Br, Hg and Pb. Trace elements Na, K, Cl, S and Cl were detected with the electron microprobe. The high sensitivity of PIXE demands a meticulous sample preparation procedure to avoid contamination problems. Practical problems associated with the application of the technique were investigated in detail. Preliminary results indicate that most trace elements except Sr, are present at close to the limits of detection at few ppm, but biologically significant data can be obtained for stock discrimination applications.

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