Anti-Inflammatory Effect of Lycii radicis in LPS-Stimulated RAW 264.7 Macrophages

The root bark of Lycium barbarum (Lycii radicis cortex, LRC) is used as a cooling agent for fever and night sweats in East Asian traditional medicine. The inhibitory effect of LRC water extract on inflammation is unknown. In this study, the anti-inflammatory effect of LRC was investigated in lipopolysaccharide (LPS)-stimulated mouse macrophage, RAW 264.7 cells. LRC extract significantly decreased the LPS-induced production of inflammatory mediators, nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokines, interleukin (IL)-1β and IL-6 in the cells. In addition, LRC extract inhibited the LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA and protein, and inflammatory cytokines mRNA in the cells. The action mechanism of LRC underlies the blocking of LPS-mediated p38 and Jun N-terminal kinase (JNK), mitogen-activated protein kinases (MAPKs), and the nuclear factor (NF)-κB signaling pathway. These results indicate that LRC extract inhibits the inflammatory response in activated macrophages by down-regulating the transcription levels of inflammatory mediators and blocking the MAPKs and NF-κB pathway.

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Anti-Inflammatory Effect of Sedum takesimense Nakai Water Extract in RAW 264.7 Cells

Korean Journal of Medicinal Crop Science ◽

10.7783/kjmcs.2016.24.3.228 ◽

2016 ◽

Vol 24 (3) ◽

pp. 228-236 ◽

Cited By ~ 1

Author(s):

Ji Hun Jang ◽

◽

Ho Kyung Jung ◽

Jae Hyung Ko ◽

Mi Ok Sim ◽

...

Keyword(s):

Water Extract ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Anti Inflammatory ◽

Inflammatory Effect

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Fermentation Improves Anti-Inflammatory Effect of Sipjeondaebotang on LPS-Stimulated RAW 264.7 Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x12500619 ◽

2012 ◽

Vol 40 (04) ◽

pp. 813-831 ◽

Cited By ~ 10

Author(s):

You-Chang Oh ◽

Won-Kyung Cho ◽

Yun Hee Jeong ◽

Ga Young Im ◽

Min Cheol Yang ◽

...

Keyword(s):

Nitric Oxide ◽

Inflammatory Mediators ◽

High Performance ◽

Biological Effects ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Tnf Α ◽

Cox 2 ◽

Anti Inflammatory

Sipjeondaebotang (SJ) has been used as a traditional drug in east-Asian countries. In this study, to provide insight into the biological effects of SJ and SJ fermented by Lactobacillus, we investigated their effects on lipopolysaccharide (LPS)-mediated inflammation in macrophages. The investigation was focused on whether SJ and fermented SJ could inhibit the production of pro-inflammatory mediators such as prostaglandin (PG) E2 and nitric oxide (NO) as well as the expressions of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB in LPS-stimulated RAW 264.7 cells. We found that SJ modestly inhibited LPS-induced PGE2, NO and TNF-α production as well as the expressions of COX-2 and iNOS. Interestingly, fermentation significantly increased its inhibitory effect on the expression of all pro-inflammatory mediators. Furthermore, fermented SJ exhibited increased inhibition of p38 MAPK and c-Jun NH2-terminal kinase (JNK) MAPK phosphorylation as well as NF-κB p65 translocation by reduced IκBα degradation compared with either untreated controls or unfermented SJ. High performance liquid chromatography (HPLC) analysis showed fermentation by Lactobacillus increases liquiritigenin and cinnamyl alcohol contained in SJ, which are known for their anti-inflammatory activities. Finally, SJ fermented by Lactobacillus exerted potent anti-inflammatory activity by inhibiting MAPK and NF-κB signaling in RAW 264.7 cells.

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The Study of Anti-inflammatory Effect of Suryeon-hwan Water Extract in RAW 264.7 Cells

The Korea Journal of Herbology ◽

10.6116/kjh.2014.29.6.125. ◽

2014 ◽

Vol 29 (6) ◽

pp. 125-132 ◽

Cited By ~ 2

Author(s):

Yeo-Hwan Yoon ◽

Sung-Bae Kim ◽

Ok-Hwa Kang ◽

Su-Hyun Mun ◽

Yun-Soo Seo ◽

...

Keyword(s):

Water Extract ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Anti Inflammatory ◽

Inflammatory Effect

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Anti-Inflammatory Effect of Ligustri Lucidi Fructus Water Extract in RAW 264.7 Cells Induced by LPS

The Journal of Korean Obstetrics and Gynecology ◽

10.15204/jkobgy.2013.26.4.066 ◽

2013 ◽

Vol 26 (4) ◽

pp. 66-81 ◽

Cited By ~ 1

Author(s):

Yong-Hyun Lee ◽

Eun-Mee Lim

Keyword(s):

Water Extract ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Anti Inflammatory ◽

Inflammatory Effect

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A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/462383 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 9

Author(s):

You-Chang Oh ◽

Won-Kyung Cho ◽

Yun Hee Jeong ◽

Ga Young Im ◽

Aeyung Kim ◽

...

Keyword(s):

Nitric Oxide ◽

Herbal Medicine ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

Prostaglandin E ◽

Raw 264.7 ◽

Dependent Manner ◽

Gelatinase Activity ◽

Anti Inflammatory ◽

Inflammatory Effect

KIOM-MA was recently reported as a novel herbal medicine effective for atopic dermatitis and asthma. In this study, we have demonstrated the inhibitory effect of KIOM-MA on proinflammatory mediator produced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. KIOM-MA significantly inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as nitric oxide (NO) and prostaglandin E2(PGE2). Consistent with the inhibitory effect on PGE2, KIOM-MA suppresses the LPS-induced migration of macrophages and gelatinase activity and the expression of matrix metalloprotease-9 (MMP-9) in a dose-dependent manner. Additionally, KIOM-MA showed a strong suppressive effect on the inflammatory cytokines production such as tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6). We also found that KIOM-MA inhibits the activation of nuclear factor-κB (NF-κB) and represses the activity of extracellular signal-regulated kinase (ERK), p38, and c-Jun NH2-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs). Taken together, we elucidated the mechanism of anti-inflammatory effect of KIOM-MA using RAW 264.7 cells stimulated by LPS.

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