COMPLEXATION OF AROMATIC MOLECULES WITH NEW CALIXARENE DERIVATIVES CONTAINING TWO ARYL SULFIDE RINGS

2005 ◽  
Vol 14 (04) ◽  
pp. 513-520
Author(s):  
DONG-MYUNG SHIN ◽  
DONG-MEE SONG ◽  
GEEWON CHUNG ◽  
KYEONGTAE KIM
Keyword(s):  
Surface Plasmon Resonance ◽  
Solid State ◽  
Molecular Recognition ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Sites ◽  
Gold Surface ◽  
Aromatic Molecules ◽  
Aryl Sulfide ◽  
Complementary Binding

The cuplike structure of calix[4]arenes is one of the most attractive features, which has been observed both in the solid state and in solution. The newly synthesized 5,11,17,23-tetrakis(3-mercaptopropyl)calix[4]arene (1) and 25,26,27,28-tetrakis(5-mercaptopenthoxy)calix[4]arene containing aryl sulfide rings (2), have four alkyl thiol linkages, which allow the calixarenes to attach onto the gold surface. Surface plasmon resonance (SPR) spectroscopy allows us to monitor the binding of calixarene derivatives on the gold surface. The 1 and 2 bind very effectively on the gold surface and self-assembled layers of 1 and 2 produce significant change in SPR signals in 30 min. Calixarenes layers are used as platforms for molecular recognition, where complementary binding sites are easily and selectively introduced. The aromatic molecules used in this study are anthracene, pyrene, coronene and rubrene. The host-guest properties of these aromatic molecules and the calixarene cavity exhibit selectivity of these aromatic molecules. The anthracene, pyrene and coronene have moderate binding affinity to the cavity, and the rubrene does not bind at all.

scholarly journals Novel aptamer to von Willebrand factor A1 domain (TAGX-0004) shows total inhibition of thrombus formation superior to ARC1779 and comparable to caplacizumab

Haematologica ◽  
2019 ◽  
Vol 105 (11) ◽  
pp. 2631-2638 ◽  
Author(s):  
Kazuya Sakai ◽  
Tatsuhiko Someya ◽  
Kaori Harada ◽  
Hideo Yagi ◽  
Taei Matsui ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Von Willebrand Factor ◽  
Plasmon Resonance ◽  
Binding Sites ◽  
Thrombus Formation ◽  
Static Conditions ◽  
A1 Domain ◽  
Von Willebrand ◽  
Willebrand Factor

von Willebrand factor (VWF) is a blood glycoprotein that plays an important role in platelet thrombus formation through interaction between its A1 domain and platelet glycoprotein Ib. ARC1779, an aptamer to the VWF A1 domain, was evaluated in a clinical trial for acquired thrombotic thrombocytopenic purpura (aTTP). Subsequently, caplacizumab, an anti-VWF A1 domain nanobody, was approved for aTTP in Europe and the United States. We recently developed a novel DNA aptamer, TAGX-0004, to the VWF A1 domain; it contains an artificial base and demonstrates high affinity for VWF. To compare the effects of these three agents on VWF A1, their ability to inhibit ristocetin- or botrocetin-induced platelet aggregation under static conditions was analyzed, and the inhibition of thrombus formation under high shear stress was investigated in a microchip flow chamber system. In both assays, TAGX-0004 showed stronger inhibition than ARC1779, and had comparable inhibitory effects to caplacizumab. The binding sites of TAGX-0004 and ARC1779 were analyzed with surface plasmon resonance performed using alanine scanning mutagenesis of the VWF A1 domain. An electrophoretic mobility shift assay showed that R1395 and R1399 in the A1 domain bound to both aptamers. R1287, K1362, and R1392 contributed to ARC1779 binding, and F1366 was essential for TAGX-0004 binding. Surface plasmon resonance analysis of the binding sites of caplacizumab identified five amino acids in the VWF A1 domain (K1362, R1392, R1395, R1399, and K1406). These results suggested that TAGX-0004 possessed better pharmacological properties than caplacizumab in vitro and might be similarly promising for aTTP treatment.

scholarly journals Improved control on the morphology and LSPR properties of plasmonic Pt NPs through enhanced solid state dewetting by using a sacrificial indium layer

RSC Advances ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 2231-2243 ◽  
Author(s):  
Sundar Kunwar ◽  
Mao Sui ◽  
Puran Pandey ◽  
Zenan Gu ◽  
Sanchaya Pandit ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Solid State ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon

Fabrication of Pt NPs with the improved configuration, spacing, uniformity and localized surface plasmon resonance (LSPR) response is demonstrated.

scholarly journals Thin SnOx films for surface plasmon resonance enhanced ellipsometric gas sensing (SPREE)

2017 ◽  
Vol 8 ◽  
pp. 522-529 ◽  
Author(s):  
Daniel Fischer ◽  
Andreas Hertwig ◽  
Uwe Beck ◽  
Volkmar Lohse ◽  
Detlef Negendank ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Gas Sensors ◽  
Tin Oxide ◽  
Plasmon Resonance ◽  
Binding Sites ◽  
Gas Sensing ◽  
Iron Doping ◽  
Actual Surface ◽  
Gas Measurement

Background: Gas sensors are very important in several fields like gas monitoring, safety and environmental applications. In this approach, a new gas sensing concept is investigated which combines the powerful adsorption probability of metal oxide conductive sensors (MOS) with an optical ellipsometric readout. This concept shows promising results to solve the problems of cross sensitivity of the MOS concept. Results: Undoped tin oxide (SnOx) and iron doped tin oxide (Fe:SnOx) thin add-on films were prepared by magnetron sputtering on the top of the actual surface plasmon resonance (SPR) sensing gold layer. The films were tested for their sensitivity to several gas species in the surface plasmon resonance enhanced (SPREE) gas measurement. It was found that the undoped tin oxide (SnOx) shows higher sensitivities to propane (C3H8) then to carbon monoxide (CO). By using Fe:SnOx, this relation is inverted. This behavior was explained by a change of the amount of binding sites for CO in the layer due to this iron doping. For hydrogen (H2) no such relation was found but the sensing ability was identical for both layer materials. This observation was related to a different sensing mechanism for H2 which is driven by the diffusion into the layer instead of adsorption on the surface. Conclusion: The gas sensing selectivity can be enhanced by tuning the properties of the thin film overcoating. A relation of the binding sites in the doped and undoped SnOx films and the gas sensing abilities for CO and C3H8 was found. This could open the path for optimized gas sensing devices with different coated SPREE sensors.

Localized and Propagating Surface Plasmon Resonance Sensors: A Study Using Carbohydrate Binding Protein

2005 ◽  
Vol 876 ◽  
Author(s):  
Chanda Yonzon ◽  
Richard P. Van Duyne
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Specific Binding ◽  
Gold Surface ◽  
Optical Biosensor ◽  
Localized Surface Plasmon ◽  
Carbohydrate Binding ◽  
Binding Studies ◽  
Assembled Monolayers

AbstractThis work encompasses a comparative analysis of the properties of two optical biosensor platforms: (1) the propagating surface plasmon resonance (SPR) sensor based on a planar, thin film gold surface and (2) the localized surface plasmon resonance (LSPR) sensor based on surface confined Ag nanoparticles fabricated by nanosphere lithography. The binding of Concanavalin A (ConA) to mannose-functionalized self-assembled monolayers (SAMs) is chosen to illustrate the similarities and the differences of these sensors. A comprehensive set of non-specific binding studies demonstrate that the single transduction mechanism is due to the specific binding of ConA to the mannose-functionalized surface. Finally, an elementary (2x1) multiplexed version of a LSPR carbohydrate sensing chip to probe the simultaneous binding of ConA to mannose and galactose-functionalized SAMs is also demonstrated.

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