Optimization of Biosynthesis of Silver Oxide Nanoparticles and Its Anticancer Activity

2017 ◽  
Vol 16 (05n06) ◽  
pp. 1750018 ◽  
Author(s):  
Vithiya Karunagaran ◽  
Kumar Rajendran ◽  
Shampa Sen

Silver oxide nanoparticle can be synthesized by chemical and biological methods. Biological synthesis has emerged as an exciting, ecofriendly approach. However, the process tends to be slow when we consider its industrial applicability. The development of reliable method for rapid synthesis of nanoparticles is one of the significant zones of interests in current nanotechnological research. In this paper, optimization of physiochemical parameters for rapid silver oxide nanoparticle synthesis using Bacillus thuringiensis SSV1 culture supernatant has been elucidated. Spherical-shaped silver oxide nanoparticles with an average particle size of 30[Formula: see text]nm were obtained. The cytotoxic effect of silver oxide nanoparticles was studied against HepG2 and Chang liver cell lines by MTT assay. These nanoparticles showed dose-dependent response on HepG2 (IC[Formula: see text]g/mL) and Chang liver cells (IC[Formula: see text]g/mL).

2021 ◽  
Author(s):  
Harshiny Muthukumar ◽  
Santosh Kumar Palanirajan ◽  
Manoj Kumar Shanmugam ◽  
Arivalagan Pugazhendhi ◽  
Sathyanarayana N. Gummadi

Abstract In this study, silver oxide nanoparticles (Ag2O-NPs) were synthesized from silver nitrate using green amaranth leaf extract as reducing agent. The degradation of caffeine and inactivation of Escherichia coli by Ag2O-NPs was studied under compact fluorescent lamp illumination irradiation. Apart from that, the antibacterial and antioxidant activities of Ag2O-NPs were also examined. Synthesized Ag2O-NPs were shaped like monodispersed husk, and cubic structured with surface area and average particle size was detected to be 100.21 (m2/g) and 81 nm respectively. Antioxidant efficacy of the Ag2O-NPs was evaluated using 1, 1-diphenyl-2-picrylhydrazyl and 91% inhibition was achieved with 100 µg Ag2O-NPs. Bacteriocidic propensity of Ag2O-NPs was examined against the S. aureus and P. aeruginosa by disc diffusion, minimum inhibitory concentration (MIC), Live and dead assay. It was observed that the NPs have higher bactericidal effect on Gram-negative as compared to Gram-positive bacteria. Up to 96% photocatalytic inactivation of E. coli was achieved using 30 µg/mL of NPs, Photocatalytic degradation of caffeine (50 ppm initial concentration) was observed to be 99% at pH 9 in 15 h using 50 mg/L of Ag2O NPs. These results indicate that Ag2O NPs can be employed in environmental applications like harmful bacteria inactivation and organic pollutants degradation.


2018 ◽  
Vol 278 ◽  
pp. 112-120 ◽  
Author(s):  
Ishaq Yusuf Habib ◽  
N.T.R.N. Kumara ◽  
Chee Ming Lim ◽  
Abdul Hanif Mahadi

A Cerium (IV) oxide nanoparticle (nanoceria) is widely used in different applications such as biomedicine and catalysis due to its unique structural, morphological and catalytic properties. In this report, the dispersion of nanoceria in both aqueous and non-aqueous (methanol and ethanol) media were studied. Adsorption-desorption processes were observed upon addition of different classes of surfactants such as citric acid (CA), cetrimonium bromide (CTAB) and diethanolamine (DEA). Stable dispersions were obtained in both aqueous, non-aqueous and electrolyte assisted media with the overall mechanism being hydrolysis, dissolution and adsorption. XRD, FE-SEM, FTIR and DLS have been used in the present study to characterize the nanoceria and to quantitatively analyze their average particle size distributions in a unique electrolyte mixture of (0.1 M NaOH/ 65% HNO3:H2O, 1:1 v/v) which has not been reported previously. The surface charge study was carried out across a wide pH range between 1.4 – 9.6 and the isoelectric points (IEP) with respect to 15 ml H2O and 50 ml H2O dispersed phases occurred at a pH of about 6.5 and 6.7 respectively. The present study could be useful in a wide range of applications including nanoparticle synthesis, stabilization, and adsorption of toxic materials, biomedical and pharmaceutical.


2021 ◽  
Author(s):  
Salah Laouini ◽  
Abderrhmane Bouafia ◽  
Mohammed Tedjani

Abstract In this study, green synthesis of silver /silver oxide nanoparticles was successfully prepared from Phoenix Dactylifera L aqueous leaves extract. The effect of different volume ratio (% v/v) (Plant extract / Precursor) on the nanoparticles silver /silver oxide nanoparticles formation, optical properties, and catalytic activity for dye degradation was studied. The obtained Ag/Ag2O nanoparticles were characterized using various techniques, such as UV-Visible, FT-IR, XRD, SEM for this purpose. The UV-Vis spectrum shows the absorption at 430 nm associated with Ag/Ag2O NPs. The optical bandgap values were found to be in the range of 3.22 to 4.47 eV for the direct bandgap and 3.73 to 5.23 eV for the indirect bandgap. The functional groups present in plant extracts were studied by FTIR. XRD confirmed the crystalline nature of Ag / Ag2O NP, and its average particle size was between 28.66-39.40 nm. SEM showed that the green synthesized silver/silver oxide nanoparticles have a spherical shape. The purpose of this study, it highlights the high catalytic activity for dye degradation of Ag/Ag2O NPs green synthesized. As a result, the use of Phoenix Dactylifera L aqueous leaves extract offers a cost-effective and eco-friendly method.


2017 ◽  
Vol 26 (4) ◽  
pp. 1025-1035 ◽  
Author(s):  
Neveen A. Salem ◽  
Mohammed A. Wahba ◽  
Wael H. Eisa ◽  
Marwa El-Shamarka ◽  
Wagdy Khalil

2021 ◽  
Vol 17 (9) ◽  
pp. 1824-1829
Author(s):  
Junlin Li ◽  
Xiangfei Li ◽  
Dong Liang ◽  
Xiaojuan Zhang ◽  
Qing Lin ◽  
...  

This study exploits the potential of zinc oxide nanoparticles (ZnO-NPs) with diverse morphologies as catalysts and antibacterial agent. Spherical ZnO-NPs, rod-shaped ZnO-NPs and flower-shaped ZnO-NPs were prepared by microemulsion method, solvent heat method and hydrothermal method, respectively. The structural characterizations of samples were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. XRD results revealed the formation of spherical ZnO-NPs, rod-shaped ZnO-NPs and flower-shaped ZnO-NPs were all wurtzite crystal structure. SEM results showed that spherical ZnO-NPs had an average particle size of 30–40 nm, rod-shaped ZnO-NPs were about 500 nm long and 100 nm wide with obvious hexagonal crystals. Flower-shaped ZnO-NPs had a three-dimensional appearance with obvious petals. Results of electrochemical HER (Hydrogen evolution reaction) experiments revealed that spherical ZnO-NPs exhibited the highest electrocatalytic activity at the lowest potential voltage due to their largest specific surface area. The antibacterial property of ZnO-NPs samples were studied by the optical density method and disc diffusion method. All samples had antibacterial effects against E. coli. and flower-shaped ZnO-NPs showed the best antibacterial activity due to the largest surface area in comparison with spherical ZnO-NPs and rod-shaped ZnO-NPs, which promised the maximum Zn2+ release as bactericide mechanism that registered in the case of different ZnO-NPs morphologies.


Biomaterials ◽  
2014 ◽  
Vol 35 (13) ◽  
pp. 4223-4235 ◽  
Author(s):  
Ang Gao ◽  
Ruiqiang Hang ◽  
Xiaobo Huang ◽  
Lingzhou Zhao ◽  
Xiangyu Zhang ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document