scholarly journals Interpreting the biochemical specificity of mouse spinal cord by confocal raman microspectral imaging

2017 ◽  
Vol 10 (05) ◽  
pp. 1743007 ◽  
Author(s):  
Yuze Gong ◽  
Zhuowen Liang ◽  
Yaning Yin ◽  
Jiwei Song ◽  
Xueyu Hu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Ex Vivo ◽  
Distribution Patterns ◽  
Chemical Components ◽  
Spinal Cord Tissue ◽  
Essential Requirement ◽  
Mouse Spinal Cord ◽  
Molecular Investigation ◽  
Specific Substance ◽  
Biochemical Mechanisms

Interpreting the biochemical specificity of spinal cord tissue is the essential requirement for understanding the biochemical mechanisms during spinal-cord-related pathological course. In this work, a longitudinal study was implemented to reveal a precise linkage between the spectral features and the molecular composition in ex vivo mouse spinal cord tissue by microspectral Raman imaging. It was testified that lipid-rich white matter could be distinguished from gray matter not only by the lipid Raman peaks at 1064, 1300, 1445 and 1660[Formula: see text]cm[Formula: see text], but also by protein (1250 and 1328[Formula: see text]cm[Formula: see text] and saccharides (913 and 1137[Formula: see text]cm[Formula: see text] distributions. [Formula: see text]-means cluster analysis was further applied to visualize the morphological basis of spinal cord tissue by chemical components and their distribution patterns. Two-dimensional chemical images were then generated to visualize the contrast between two different tissue types by integrating the intensities of the featured Raman bands. All the obtained results illustrated the biochemical characteristics of spinal cord tissue, as well as some specific substance variances between different tissue types, which formed a solid basis for the molecular investigation of spinal cord pathological alterations.

Confocal raman microspectral imaging of ex vivo human spinal cord tissue

2016 ◽  
Vol 163 ◽  
pp. 177-184 ◽  
Author(s):  
Shuang Wang ◽  
Zhuowen Liang ◽  
Yuzhe Gong ◽  
Yaning Yin ◽  
Kaige Wang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Ex Vivo ◽  
Spinal Cord Tissue ◽  
Human Spinal Cord ◽  
Confocal Raman

scholarly journals An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (2) ◽  
Author(s):  
Juan Garré ◽  
Guang Yang ◽  
Feliksas Bukauskas ◽  
Michael Bennett
Keyword(s):  
Spinal Cord ◽  
Ex Vivo ◽  
Glial Activation ◽  
Slice Preparation ◽  
Mouse Spinal Cord ◽  
Spinal Cord Slice

scholarly journals Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (18) ◽  
Author(s):  
Mariko Kinoshita-Kawada ◽  
Hiroshi Hasegawa ◽  
Tsunaki Hongu ◽  
Shigeru Yanagi ◽  
Yasunori Kanaho ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Gene Transfer ◽  
Ex Vivo ◽  
Explant Culture ◽  
Embryonic Mouse ◽  
Mouse Spinal Cord

scholarly journals Assessment of axonal recruitment using model-guided preclinical spinal cord stimulation in the ex vivo adult mouse spinal cord

2019 ◽  
Vol 122 (4) ◽  
pp. 1406-1420 ◽  
Author(s):  
Shaquia Idlett ◽  
Mallika Halder ◽  
Tianhe Zhang ◽  
Jorge Quevedo ◽  
Natalie Brill ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Finite Element ◽  
Spinal Cord Stimulation ◽  
Adult Mouse ◽  
Ex Vivo ◽  
Dorsal Column ◽  
Primary Afferents ◽  
Mouse Spinal Cord ◽  
C Fibers ◽  
Postsynaptic Dorsal Column

Spinal cord stimulation (SCS) is used clinically to limit chronic pain, but fundamental questions remain on the identity of axonal populations recruited. We developed an ex vivo adult mouse spinal cord preparation to assess recruitment following delivery of clinically analogous stimuli determined by downscaling a finite element model of clinical SCS. Analogous electric field distributions were generated with 300-µm × 300-µm electrodes positioned 200 µm above the dorsal column (DC) with stimulation between 50 and 200 µA. We compared axonal recruitment using electrodes of comparable size and stimulus amplitudes when contacting the caudal thoracic DC and at 200 or 600 μm above. Antidromic responses recorded distally from the DC, the adjacent Lissauer tract (LT), and in dorsal roots (DRs) were found to be amplitude and site dependent. Responses in the DC included a unique component not seen in DRs, having the lowest SCS recruitment amplitude and fastest conduction velocity. At 200 μm above, mean cathodic SCS recruitment threshold for axons in DRs and LT were 2.6 and 4.4 times higher, respectively, than DC threshold. SCS recruited primary afferents in all (up to 8) caudal segments sampled. Whereas A and C fibers could be recruited at nearby segments, only A fiber recruitment and synaptically mediated dorsal root reflexes were observed in more distant (lumbar) segments. In sum, clinically analogous SCS led to multisegmental recruitment of several somatosensory-encoding axonal populations. Most striking is the possibility that the lowest threshold recruitment of a nonprimary afferent population in the DC are postsynaptic dorsal column tract cells (PSDCs) projecting to gracile nuclei. NEW & NOTEWORTHY Spinal cord stimulation (SCS) is used clinically to control pain. To identify axonal populations recruited, finite element modeling identified scaling parameters to deliver clinically analogous SCS in an ex vivo adult mouse spinal cord preparation. Results showed that SCS first recruited an axonal population in the dorsal column at a threshold severalfold lower than primary afferents. These putative postsynaptic dorsal column tract cells may represent a previously unconsidered population responsible for SCS-induced paresthesias necessary for analgesia.

Antisera to different glycolipids induce myelin alterations in mouse spinal cord tissue cultures

1985 ◽  
Vol 339 (1) ◽  
pp. 9-18 ◽  
Author(s):  
German A. Roth ◽  
Matias Röyttä ◽  
Robert K. Yu ◽  
Cedric S. Raine ◽  
Murray B. Bornstein
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Tissue ◽  
Tissue Cultures ◽  
Mouse Spinal Cord

scholarly journals Basal Lamina Formation by Astrocytes in Organotypic Cultures of Mouse Spinal Cord Tissue

1985 ◽  
Vol 44 (3) ◽  
pp. 295-303 ◽  
Author(s):  
HIROFUMI KUSAKA ◽  
ASAO HlRANO ◽  
Murray B. Bornstein ◽  
Cedric S. Raine
Keyword(s):  
Spinal Cord ◽  
Basal Lamina ◽  
Spinal Cord Tissue ◽  
Organotypic Cultures ◽  
Mouse Spinal Cord
Sign in / Sign up

Export Citation Format

Share Document