Control of Protein Crystal Nucleation and Growth Using Stirring Solution

This paper reviews investigations on protein crystallization. It aims to present a comprehensive rather than complete account of recent studies and efforts to elucidate the most intimate mechanisms of protein crystal nucleation. It is emphasized that both physical and biochemical factors are at play during this process. Recently-discovered molecular scale pathways for protein crystal nucleation are considered first. The bond selection during protein crystal lattice formation, which is a typical biochemically-conditioned peculiarity of the crystallization process, is revisited. Novel approaches allow us to quantitatively describe some protein crystallization cases. Additional light is shed on the protein crystal nucleation in pores and crevices by employing the so-called EBDE method (equilibration between crystal bond and destructive energies). Also, protein crystal nucleation in solution flow is considered.

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Beneficial Effect of Solubility Enhancers on Protein Crystal Nucleation and Growth

Langmuir ◽

10.1021/la803185m ◽

2009 ◽

Vol 25 (8) ◽

pp. 4579-4587 ◽

Cited By ~ 11

Author(s):

Rajendrakumar A. Gosavi ◽

Venkateswarlu Bhamidi ◽

Sasidhar Varanasi ◽

Constance A. Schall

Keyword(s):

Beneficial Effect ◽

Nucleation And Growth ◽

Crystal Nucleation ◽

Protein Crystal ◽

Solubility Enhancers

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Raman Spectroscopy of Protein Crystal Nucleation and Growth

American Journal of Biochemistry and Biotechnology ◽

10.3844/ajbbsp.2014.202.207 ◽

2014 ◽

Vol 10 (3) ◽

pp. 202-207 ◽

Cited By ~ 1

Author(s):

Pechkova Eugenia ◽

Parshina Evgenia ◽

Maksimov Evgenii ◽

Kutusov Nikolai ◽

Brazhe Nadezda ◽

...

Keyword(s):

Raman Spectroscopy ◽

Nucleation And Growth ◽

Crystal Nucleation ◽

Protein Crystal

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Advancements (and challenges) in the study of protein crystal nucleation and growth; thermodynamic and kinetic explanations and comparison with small-molecule crystallization

Progress in Crystal Growth and Characterization of Materials ◽

10.1016/j.pcrysgrow.2020.100484 ◽

2020 ◽

Vol 66 (2) ◽

pp. 100484 ◽

Cited By ~ 1

Author(s):

Christo N. Nanev

Keyword(s):

Small Molecule ◽

Nucleation And Growth ◽

Crystal Nucleation ◽

Protein Crystal

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Kinetics of protein crystal nucleation and growth in the batch method

Journal of Crystal Growth ◽

10.1016/s0022-0248(98)00839-2 ◽

1999 ◽

Vol 196 (2-4) ◽

pp. 220-225 ◽

Cited By ~ 20

Author(s):

James K. Baird ◽

Susan C. Hill ◽

John C. Clunie

Keyword(s):

Nucleation And Growth ◽

Crystal Nucleation ◽

Protein Crystal ◽

Batch Method ◽

Kinetics Of

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Molecular Dynamics Simulations of Crystal Nucleation near Interfaces in Incompatible Polymer Blends

Polymers ◽

10.3390/polym13030347 ◽

2021 ◽

Vol 13 (3) ◽

pp. 347

Author(s):

Wenlin Zhang ◽

Lingyi Zou

Keyword(s):

Molecular Dynamics ◽

Polymer Blends ◽

Md Simulations ◽

Nucleation And Growth ◽

Crystal Nucleation ◽

Crystalline Order ◽

Mobile Species ◽

Deep Supercooling ◽

Crystallizable Polymer ◽

Dynamics Simulations

We apply molecular dynamics (MD) simulations to investigate crystal nucleation in incompatible polymer blends under deep supercooling conditions. Simulations of isothermal nucleation are performed for phase-separated blends with different degrees of incompatibility. In weakly segregated blends, slow and incompatible chains in crystallizable polymer domains can significantly hinder the crystal nucleation and growth. When a crystallizable polymer is blended with a more mobile species in interfacial regions, enhanced molecular mobility leads to the fast growth of crystalline order. However, the incubation time remains the same as that in pure samples. By inducing anisotropic alignment near the interfaces of strongly segregated blends, phase separation also promotes crystalline order to grow near interfaces between different polymer domains.

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