Reconstructing distances in physical maps of chromosomes with nonoverlapping probes

2000 ◽  
Author(s):  
John Krececioglu ◽  
Sanjay Shete ◽  
Jonathan Arnold
Keyword(s):  
Physical Maps

scholarly journals Heterogeneity in Rates of Recombination Across the Mouse Genome

Genetics ◽  
1996 ◽  
Vol 142 (2) ◽  
pp. 537-548 ◽  
Author(s):  
Michael W Nachman ◽  
Gary A Churchill
Keyword(s):  
Linkage Map ◽  
Genetic Map ◽  
Large Scale ◽  
Physical Map ◽  
Genetic Maps ◽  
Recombination Rates ◽  
Physical Maps ◽  
Genomic Patterns ◽  
Mary Lyon ◽  
Microsatellite Linkage

Abstract If loci are randomly distributed on a physical map, the density of markers on a genetic map will be inversely proportional to recombination rate. First proposed by MARY LYON, we have used this idea to estimate recombination rates from the Drosophila melanogaster linkage map. These results were compared with results of two other studies that estimated regional recombination rates in D. melanogaster using both physical and genetic maps. The three methods were largely concordant in identifying large-scale genomic patterns of recombination. The marker density method was then applied to the Mus musculus microsatellite linkage map. The distribution of microsatellites provided evidence for heterogeneity in recombination rates. Centromeric regions for several mouse chromosomes had significantly greater numbers of markers than expected, suggesting that recombination rates were lower in these regions. In contrast, most telomeric regions contained significantly fewer markers than expected. This indicates that recombination rates are elevated at the telomeres of many mouse chromosomes and is consistent with a comparison of the genetic and cytogenetic maps in these regions. The density of markers on a genetic map may provide a generally useful way to estimate regional recombination rates in species for which genetic, but not physical, maps are available.

scholarly journals Xenopus Cytogenetics and Chromosomal Evolution

2015 ◽  
Vol 145 (3-4) ◽  
pp. 192-200 ◽  
Author(s):  
Vladimir Krylov ◽  
Tereza Tlapakova
Keyword(s):  
Developmental Biology ◽  
Genetic Linkage ◽  
Chromosome Banding ◽  
Chromosomal Evolution ◽  
Model Organisms ◽  
Gene Localization ◽  
Physical Maps ◽  
Gene Position ◽  
Tightly Coupled

The genus Xenopus represents important model organisms in the field of developmental biology and chromosomal evolution. Developmental processes are tightly coupled with the analysis of gene function via genetic linkage and mapping. Cytogenetic techniques such as chromosome banding or FISH are essential tools for the determination of gene position and subsequently for the construction of linkage and physical maps. Here, we present a summary of key achievements in X. tropicalis and X. laevis cytogenetics with emphasis on the gene localization to chromosomes. The second part of this review is focused on the chromosomal evolution regarding both above-mentioned species. With respect to methodology, hybridization techniques such as FISH and chromosome-specific painting FISH are highlighted.

Integrated physical maps of 2DL, 6BS and 7DL carrying loci for grain protein content and pre-harvest sprouting tolerance in bread wheat

2001 ◽  
Vol 29 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
R. K. Varshney ◽  
M. Prasad ◽  
J. K. Roy ◽  
M. S. Röder ◽  
H. S. Balyan ◽  
...  
Keyword(s):  
Protein Content ◽  
Bread Wheat ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Physical Maps

scholarly journals Cytochrome P450 monooxygenase genes in the wild silkworm, Bombyx mandarina

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10818
Author(s):  
Linrong Wan ◽  
Anlian Zhou ◽  
Wenfu Xiao ◽  
Bangxing Zou ◽  
Yaming Jiang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Enrichment Analysis ◽  
Silk Gland ◽  
P450 Monooxygenase ◽  
Protein Coding ◽  
Bombyx Mandarina ◽  
Physical Maps ◽  
Cytochrome P450 Genes ◽  
In The Wild ◽  
Genome Assemblies

Wild (Bombyx mandarina) and domestic silkworms (B. mori) are good models for investigating insect domestication, as 5000 years of artificial breeding and selection have resulted in significant differences between B. mandarina and B. mori. In this study, we improved the genome assemblies to the chromosome level and updated the protein-coding gene annotations for B. mandarina. Based on this updated genome, we identified 68 cytochrome P450 genes in B. mandarina. The cytochrome P450 repository in B. mandarina is smaller than in B. mori. Certain currently unknown key genes, rather than gene number, are critical for insecticide resistance in B. mandarina, which shows greater resistance to insecticides than B. mori. Based on the physical maps of B. mandarina, we located 66 cytochrome P450s on 18 different chromosomes, and 27 of the cytochrome P450 genes were concentrated into seven clusters. KEGG enrichment analysis of the P450 genes revealed the involvement of cytochrome P450 genes in hormone biosynthesis. Analyses of the silk gland transcriptome identified candidate cytochrome P450 genes (CYP306A) involved in ecdysteroidogenesis and insecticide metabolism in B. mandarina.

[57] Genetic and physical maps of Saccharomyces cerevisiae

1991 ◽  
pp. 827-863 ◽  
Author(s):  
Robert K. Mortimer ◽  
David Schild ◽  
C.Rebecca Contopoulou ◽  
Jonathan A. Kans
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Physical Maps ◽  
Genetic And Physical Maps

Single stranded nucleic acid binding structures on chicken lampbrush chromosomes

1995 ◽  
Vol 108 (4) ◽  
pp. 1391-1396
Author(s):  
I. Solovei ◽  
H. Macgregor ◽  
E. Gaginskaya
Keyword(s):  
Nucleic Acid ◽  
Structural Organization ◽  
Mrna Processing ◽  
Nucleic Acid Binding ◽  
Lampbrush Chromosomes ◽  
Cytological Evidence ◽  
Rna Transcripts ◽  
Physical Maps ◽  
Distinctive Morphology ◽  
Lampbrush Loops

In chicken oocytes, proteins of the K/J family or their analogs, such as are known to be involved in mRNA processing in humans, are closely associated with nascent C-rich RNA transcripts on the loops of lampbrush chromosomes. Using labelled single stranded nucleotide probes and an antibody to protein K, these C-rich transcripts have been mapped to six different pairs of lampbrush loops situated on 3 macrochromosomes, the sex bivalent (ZW) and certain microchromosomes. Each of these loop pairs has a distinctive morphology. The observations represent cytological evidence of the connection between K-proteins and C-rich RNA transcripts. Another structure, the spaghetti marker of macrochromosome II, also preferentially binds C-rich homonucleotides. This spaghetti marker has a highly distinctive fine structural organization that is quite unlike that of lampbrush loops. Its proteins are not recognised by antibodies to protein K. Homonucleotide binding loops are recommended as potentially extremely valuable as markers on physical maps of chicken chromosomes.

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