High-density physical maps reveal that the dominant male-sterile gene Ms3 is located in a genomic region of low recombination in wheat and is not amenable to map-based cloning

2001 ◽  
Vol 103 (6-7) ◽  
pp. 998-1006 ◽  
Author(s):  
L. L. Qi ◽  
B. S. Gill
Keyword(s):  
Genomic Region ◽  
High Density ◽  
Dominant Male ◽  
Male Sterile ◽  
Physical Maps

scholarly journals QTL Mapping of Quality Related Traits in Peanut Using Whole-Genome Resequencing

2021 ◽  
Author(s):  
Ziqi Sun ◽  
Feiyan Qi ◽  
Hua Liu ◽  
Li Qin ◽  
Jing Xu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Protein Content ◽  
Genomic Region ◽  
High Density ◽  
Whole Genome ◽  
Missense Mutations ◽  
Genome Resequencing ◽  
Phenotypic Effect ◽  
Whole Genome Resequencing

Abstract Background: Oil and protein content, as well as fatty acid composition, are important quality traits in peanut. Elucidating the genetic mechanisms underlying these traits may help researchers to obtain improved cultivars through molecular breeding techniques.Results: Whole-genome resequencing of an RIL population of 318 lines was performed to construct a high-density linkage map and identify QTLs for peanut quality. The map, containing 4561 bin markers, covered a length of 2032.39 cM with an average marker density of 0.45 cM. A total of 109 QTLs for oil content, protein content, and fatty acid compositions were mapped on the 18 peanut chromosomes. The QTL qA05.1 was detected in four different environments and exhibited a major phenotypic effect on the content of oil, proteins, and six fatty acids. The genomic region spanned by qA05.1, corresponding to a physical interval of approximately 1.50 Mb, contains two polymorphic SNPs between two parents that could cause missense mutations. The two SNP sites were employed as KASP markers and validated using lines with extremely high and low oil contents; these sites may be useful in the marker-assisted breeding of peanut varieties with high oil contents.Conclusions: A high-density genetic map with 4561 bin markers was constructed, and a major and pleiotropic QTL located on LG05 was stably detected for oil, protein and fatty acids across four different environments.

Soft Red Winter Wheat Germplasm Segregating for a Dominant Male Sterile Gene

Crop Science ◽  
1983 ◽  
Vol 23 (5) ◽  
pp. 1022-1022
Author(s):  
P. S. Baenziger ◽  
D. J. Sammons ◽  
D. H. Smith
Keyword(s):  
Winter Wheat ◽  
Dominant Male ◽  
Male Sterile ◽  
Wheat Germplasm ◽  
Soft Red Winter Wheat ◽  
Red Winter Wheat

Effects of recurrent selection on populations of various generations in wheat by using the Tai Gu single dominant male-sterile gene

1996 ◽  
Vol 126 (4) ◽  
pp. 397-402 ◽  
Author(s):  
X. W. Wang ◽  
J. R. Lap ◽  
L Fan ◽  
R. B. Zhang
Keyword(s):  
Multivariate Analysis ◽  
Recurrent Selection ◽  
Dominant Gene ◽  
Dominant Male ◽  
Mass Selection ◽  
Base Population ◽  
Male Sterile ◽  
Single Character ◽  
Parental Lines ◽  
Selection Of

SUMMARYMass selection (MS) and bulk selection of a single character (CS) were used for the improvement of a base population, Bl, which was composed of Tai Gu male-sterile progenies of ten high protein content and four high yielding parental lines of wheat (Triticum aestivum L.), transferred by 6–10 backcrossing generations, with equal numbers of plants from each line. Backcross mass selection (BS) was used for the improvement of a second base population, B2, which was formed by Tai Gu malesterile plants of four high yielding parental lines, again with equal numbers of plants from each line. Three cycles of selection were conducted for both population Bl and B2. Direct genetic advances of sterile plants for six traits were studied. The results indicated that use of the Tai Gu male-sterile single dominant gene is effective for recurrent selection of wheat. For improvement of a single character, CS was the most effective method, of which the selective effect in the first cycle (Cl) was greater than in the second (C2) and the third cycle (C3), although multivariate analysis snowed that the CS method was not conducive to the maintenance of variation in a population. The greatest response to selection was found in C2 with the MS method. Multivariate analysis also indicated that both the MS and BS methods increased the variation in the population, but MS was found to be the best selective method as judged by the mean response over three cycles of selection.

Identification and Analysis of Zbs1, a Dominant Male-Sterile Mutant of Naked Oat (Avena nudaL.)

Crop Science ◽  
2016 ◽  
Vol 56 (4) ◽  
pp. 1423-1428
Author(s):  
Cai Yang ◽  
Haitao Zhou ◽  
Xiao Shi ◽  
Xinjun Zhang ◽  
Tianliang Li ◽  
...  
Keyword(s):  
Dominant Male ◽  
Male Sterile ◽  
Naked Oat ◽  
Sterile Mutant

Fertility behaviour of rice (Oryza sativa) lines with dominant male sterile gene and inheritance of sterility and fertility restoration

2006 ◽  
Vol 98 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Haohua He ◽  
Xiaoshong Peng ◽  
Huiming Gong ◽  
Changlan Zhu ◽  
Guoyou Ye
Keyword(s):  
Oryza Sativa ◽  
Fertility Restoration ◽  
Dominant Male ◽  
Male Sterile ◽  
Fertility Behaviour

scholarly journals Two types of genetic interaction implicate the whirligig gene of Drosophila melanogaster in microtubule organization in the flagellar axoneme.

Genetics ◽  
1990 ◽  
Vol 126 (4) ◽  
pp. 961-973 ◽  
Author(s):  
L L Green ◽  
N Wolf ◽  
K L McDonald ◽  
M T Fuller
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Interaction ◽  
Dominant Male ◽  
Loss Of Function ◽  
Male Sterile ◽  
Microtubule Organization ◽  
Beta Tubulin ◽  
Absolute Level ◽  
Alpha Tubulin ◽  
Flagellar Axoneme

Abstract The mutant nc4 allele of whirligig (3-54.4) of Drosophila melanogaster fails to complement mutations in an alpha-tubulin locus, alpha 1t, mutations in a beta-tubulin locus, B2t, or a mutation in the haywire locus. However, wrl fails to map to any of the known alpha- or beta-tubulin genes. The extragenic failure to complement could indicate that the wrl product participates in structural interactions with microtubule proteins. The whirligig locus appears to be haploinsufficient for male fertility. Both a deficiency of wrl and possible loss of function alleles obtained by reverting the failure to complement between wrlnc4 and B2tn are dominant male sterile in a genetic background wild type for tubulin. The dominant male sterility of the revertant alleles is suppressed if the flies are also heterozygous for B2tn, for a deficiency of alpha 1t, or for the haync2 allele. These results suggest that it is not the absolute level of wrl gene product but its level relative to tubulin or microtubule function that is important for normal spermatogenesis. The phenotype of homozygous wrl mutants suggests that the whirligig product plays a role in postmeiotic spermatid differentiation, possibly in organizing the microtubules of the sperm flagellar axoneme. Flies homozygous for either wrlnc4 or revertant alleles are viable and female fertile but male sterile. Premeiotic and meiotic stages of spermatogenesis appear normal. However, in post-meiotic stages, flagellar axonemes show loss of the accessory microtubule on the B-subfiber of outer doublet microtubules, outer triplet instead of outer doublet microtubules, and missing central pair microtubules.

scholarly journals Identification and High-Density Mapping of Gene-Rich Regions in Chromosome Group 1 of Wheat

Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1883-1891 ◽  
Author(s):  
Kulvinder S Gill ◽  
Bikram S Gill ◽  
Takashi R Endo ◽  
Teri Taylor
Keyword(s):  
Genetic Map ◽  
Physical Map ◽  
Average Distance ◽  
High Density ◽  
Genetic Maps ◽  
Cdna Clones ◽  
Density Mapping ◽  
Physical Maps ◽  
Group 1 Chromosomes ◽  
Group 1

We studied the distribution of genes and recombination in wheat (Triticum aestivum) group 1 chromosomes by comparing high-density physical and genetic maps. Physical maps of chromosomes 1A, 1B, and 1D were generated by mapping 50 DNA markers on 56 single-break deletion lines. A consensus physical map was compared with the 1D genetic map of Triticum tauschii (68 markers) and a Triticeae group 1 consensus map (288 markers) to generate a cytogenetic ladder map (CLM). Most group 1 markers (86%) were present in five clusters that encompassed only 10% of the group 1 chromosome. This distribution may reflect that of genes because more than half of the probes were cDNA clones and 30% were PstI genomic. All 14 agronomically important genes in group 1 chromosomes were present in these clusters. Most recombination occurred in gene-cluster regions. Markers fell at an average distance of 244 kb in these regions. The CLM involving the Triticeae consensus genetic map revealed that the above distribution of genes and recombination is the same in other Triticeae species. Because of a significant number of common markers, our CLM can be used for comparative mapping and to estimate physical distances among markers in many Poaceae species including rice and maize.

Genetic analysis and mapping of a thermosensitive genic male sterility gene, tms6(t), in rice (Oryza sativa L.)

Genome ◽  
2010 ◽  
Vol 53 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Xia Liu ◽  
Xihong Li ◽  
Xin Zhang ◽  
Songwen Wang
Keyword(s):  
Genetic Analysis ◽  
Molecular Mapping ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Male Sterile ◽  
Chromosome 10 ◽  
Physical Maps ◽  
Specific Temperature ◽  
Simple Sequence

Thermosensitive genic male-sterile (TGMS) rice is widely used in hybrid rice production. Because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered TGMS line, G20S, has an opposite phenotype compared with normal TGMS lines. G20S is completely sterile when the temperature is lower than 29.5 °C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility of G20S was controlled by a single recessive gene that was named tms6(t). An F2 population from the cross between G20S and Jing226 was developed and used for molecular mapping of the tms6(t) gene. The simple sequence repeat technique, combined with bulked segregant analysis, was used to screen markers linked to the target gene, and linkage markers such as RM216, RM2504, RM3152, and RM4455 were identified. These markers are all located on chromosome 10; therefore, tms6(t) was subsequently mapped on chromosome 10. Co-segregation analysis using the F2 population showed that two of these markers, RM3152 and RM4455, were closely linked to tms6(t) at distances of 3.00 cM and 1.10 cM, respectively. Additionally, integration of the genetic and physical maps showed that there were 15 BAC/PAC clones overlapping between RM3152 and RM4455, and tms6(t) was mapped to an interval of 1455 kb.

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