Membrane permeability of isolated lung cells to nonelectrolytes at different temperatures

1982 ◽  
Vol 243 (5) ◽  
pp. C285-C292 ◽  
Author(s):  
R. A. Garrick ◽  
F. P. Chinard
Keyword(s):  
Membrane Permeability ◽  
Tritiated Water ◽  
Cellular Systems ◽  
Lung Cells ◽  
Cell Preparation ◽  
Rabbit Lung ◽  
Novikoff Hepatoma ◽  
Permeability Coefficients ◽  
Diffusional Permeability ◽  
Isolated Lung

Membrane permeability coefficients (P0) of rabbit lung cells consisting primarily of alveolar epithelial and endothelial cells and of alveolar macrophages from dog lungs were determined for tritiated water, n-[14C]alcohols, and [14C]antipyrine over the temperature range 10 to 37 degrees C with the series-parallel pathway model. In the mixed cell preparation both the diffusional permeability to water (755 X 10(-5) cm.s-1 at 37 degrees C) and the response to temperature change (apparent activation energy, Ea, 10 kcal.mol-1) are greater than the corresponding values in the macrophages (110 X 10(-5) cm.s-1 and 4.8 kcal.mol-1, respectively). The permeability coefficients for the small alcohols (C1-C3) are similar and considerably higher than for water in both cellular preparations. The values of the permeability coefficients and the temperature dependence for antipyrine and the larger alcohols in the mixed lung cells differ from the values obtained in the macrophages. Comparison of our results with those obtained in erythrocytes and Novikoff hepatoma cells demonstrates the differences in water permeability in each cell preparation and the similarity in permeation for the more lipophilic solutes in the cell preparations. These differences may be important in the comparison of results obtained in isolated cellular systems and in intact tissues and organs.

Membrane permeability of isolated lung cells to nonelectrolytes

1977 ◽  
Vol 233 (3) ◽  
pp. C104-C110 ◽  
Author(s):  
R. A. Garrick ◽  
W. R. Redwood
Keyword(s):  
Tritiated Water ◽  
Small Diameter ◽  
Extracellular Fluid ◽  
Enzymatic Digestion ◽  
Lung Cells ◽  
Rabbit Lung ◽  
Permeability Coefficients ◽  
Isolated Lung ◽  
Diffusional Model ◽  
Polyethylene Tubing

Mixtures of viable endothelial and epithelial cells were separated by enzymatic digestion from rabbit lung and recovered by centrifugation. The cells were mixed with an extracellular marker and packed by centrifugation into small-diameter polyethylene tubing and pulsed with tritiated water and 14C-labeled alcohols. Calculation of diffusion coefficients for the packed cell column (D), intracellular material (D2), and extracellular fluid (D1) was based on a local steady-state one-dimensional diffusional model. Permeability coefficients were: tritiated water, 288 X 10(-5) cm s-1; methanol, 385 X 10(-5) cm s-1; ethanol, 214 X 10(-5) cm s-1; propanol, 277 X 10(-5) cm s-1; and hexanol, 1255 X 10(-5) cm s-1. The permeability coefficients of these aliphatic alcohols show a minimum at ethanol with hexanol having the highest value of all substances tested. The results support the concept of parallel aqueous and lipid pathways for small solutes in the plasma membrane. Study of the permeability properties of isolated lung cells can provide information on the cellular pathway in the transcapillary transport of water and solutes in the lung.

Use of isolated lung cells in pulmonary toxicology

1993 ◽  
Vol 7 (4) ◽  
pp. 359-364 ◽  
Author(s):  
B. Nemery ◽  
P.H.M. Hoet
Keyword(s):  
Lung Cells ◽  
Isolated Lung

Triiodothyronine Nuclear Binding in Fetal and Adult Rabbit Lung and Cultured Lung Cells*

Endocrinology ◽  
1978 ◽  
Vol 103 (5) ◽  
pp. 1725-1731 ◽  
Author(s):  
JEFFREY A. LINDENBERG ◽  
ARLETTE BREHIER ◽  
PHILIP L. BALLARD
Keyword(s):  
Adult Rabbit ◽  
Lung Cells ◽  
Rabbit Lung ◽  
Nuclear Binding

Leucocytes and Platelets in Isolated Lung Perfusion

1975 ◽  
Author(s):  
N. van Zandwijk ◽  
Th. F. J. Lenssen ◽  
Elisabeth M. Prakke ◽  
J. van der Meer ◽  
A. S. Groen ◽  
...  
Keyword(s):  
Whole Blood ◽  
Serotonin Level ◽  
Rabbit Lung ◽  
Isolated Lung Perfusion ◽  
Isolated Lung ◽  
Isolated Lungs ◽  
Almost All ◽  
A Site ◽  
Plasma Serotonin ◽  
Lung Preparation

Removal of leucocytes and platelets from homologous blood prevents early functional and morphologic deterioration of an isolated rabbit lung preparation. Lungs perfused with whole blood have a marked ability for granulocyte sequestration. Control experiments in which the perfusion system was not connected with the lung revealed an increase of plasma serotonin and histamine levels due to platelet injury and/or aggregation generated by the perfusion circuit. When an isolated lung was connected with the circuit, plasma serotonin was largely metabolized. However, the metabolic uptake of serotonin by lungs perfused with whole blood decreased after 30 min while pulmonary vascular resistence (PVR) increased. In contrast, perfusion experiments with blood from which almost all leucocytes had been removed, were characterized by a less pronounced rise of plasma serotonin level and no or little change in PVR.It is concluded that leucocytes particularly granulocytes most likely traumatized by the extracorporeal procedure, have a detrimental effect on the integrity of perfused isolated lungs. Damage of endothelium, which has been recognized as a site of serotonin metabolism, could then be an early symptom. The results of our experiments in which the perfused isolated lung preparation has been used as a model for pulmonary dysfunction after extracorporeal circulation and massive transfusion warrants more attention to be paid to the role granulocytes and platelets play in the genesis of respiratory distress after such procedures.

Volcanic ash: Toxicity to isolated lung cells

1982 ◽  
Vol 9 (2) ◽  
pp. 317-325 ◽  
Author(s):  
V. Castranova ◽  
L. Bowman ◽  
J. M. Shreve ◽  
G. S. Jones ◽  
P. R. Miles
Keyword(s):  
Volcanic Ash ◽  
Lung Cells ◽  
Isolated Lung

scholarly journals In vitrotoxicity of indoor fungi from dwellings in Slovakia: testing on the isolated lung cells

2008 ◽  
Author(s):  
Z. Kováčiková ◽  
E. Tátrai ◽  
E. Piecková ◽  
Z. Kolláriková ◽  
V. Jančinová ◽  
...  
Keyword(s):  
Lung Cells ◽  
Isolated Lung ◽  
Indoor Fungi

A Study of Diffusional Permeability of Water, Sodium and Chloride in Yolk-Sac Larvae of Cod (Gadus Morhua L.)

1989 ◽  
Vol 147 (1) ◽  
pp. 125-132 ◽  
Author(s):  
PETER TYTLER ◽  
MICHAEL V. BELL
Keyword(s):  
Gadus Morhua ◽  
Sea Water ◽  
Fish Larvae ◽  
Yolk Sac ◽  
Adult Fish ◽  
Permeability Coefficients ◽  
Osmotic Permeability ◽  
Marine Fish Larvae ◽  
Diffusional Permeability ◽  
Previous Hypothesis

1. The fluxes of 3H2O, 22Na and 36Cl were simultaneously measured in yolk-sac larvae of cod (Gadus morhua L.) in 34‰ sea water at 4.5°C. 2. The rates of turnover of all three isotopes were higher than in adult fish. Diffusional permeability coefficients, which relate ion fluxes to surface area, were however lower, indicating that larvae are less permeable than adults. Furthermore, there is close agreement between the diffusional and osmotic permeability coefficients, which supports a previous hypothesis that relatively low drinking rates in marine fish larvae are a consequence of low integumental permeability. 3. Estimates of the sodium and chloride concentrations derived from the equilibrium levels of 22Na, 36Cl and 3H2O indicate that yolk-sac larvae of cod regulate their body fluids hypotonic to sea water. Also, the ionic concentrations of the tissues of yolk-sac cod larvae are similar to those of adults.

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