The “calcium clamp”: effect of constant hypocalcemia on parathyroid hormone secretion

1981 ◽  
Vol 240 (6) ◽  
pp. E649-E655
Author(s):  
J. Fox ◽  
H. Heath
Keyword(s):  
Parathyroid Hormone ◽  
Feedback Control ◽  
Rapid Determination ◽  
Hormone Secretion ◽  
Conscious Dogs ◽  
Half Time ◽  
Experimental Animals ◽  
A New Technique ◽  
Ipth Concentration

This report describes acute studies of parathyroid hormone (PTH) secretion and metabolism in conscious dogs, performed with a new technique, the “calcium clamp.” Bolus injections and graded infusions of either calcium (Ca) or EGTA, respectively, increase or decrease plasma Ca to desired levels in 1–2 min; rapid determination of plasma Ca permits feedback control of the infusion rates to maintain the desired Ca concentration for prolonged periods. Using this technique, we have examined the effect in five dogs of a sustained (1 h) decrease in plasma Ca from 9.6 to 7.6 mg/dl on the secretion of PTH. Plasma immunoreactive PTH (IPTH) concentration in precaval blood increased within 1 min, peaked at 4–10 min (greater than 5 times control), but thereafter declined gradually to 57% of the maximum at 60 min, despite ongoing and constant hypocalcemia. Abrupt restoration of normocalcemia caused IPTH levels to decrease with an apparent half-time of 3.0 +/- 0.3 min (mean +/- SE). Thus, external feedback-regulated control of plasma Ca is possible in experimental animals. IPTH concentrations decline from the maximum during constant hypocalcemia, a new observation that suggests that PTH secretion and/or metabolism are altered progressively by the hypocalcemia.

Episodic secretion of parathyroid hormone in the dog

1981 ◽  
Vol 241 (3) ◽  
pp. E171-E177
Author(s):  
J. Fox ◽  
K. P. Offord ◽  
H. Heath
Keyword(s):  
Spectral Analysis ◽  
Parathyroid Hormone ◽  
Conscious Dogs ◽  
Blood Samples ◽  
Pentobarbital Anesthesia ◽  
Ipth Concentration

This study was designed to determine whether parathyroid hormone (PTH) is secreted episodically, to characterize any such rhythms, and to see whether the rhythms can be altered by stimulating PTH secretion using constant hypocalcemia. We collected blood samples at 1-min intervals for 1 h from the precava or postcava of conscious dogs during normocalcemia or induced, constant hypocalcemia. In two anesthetized normocalcemic dogs we catheterized a caudal thyroid vein and collected all the effluent blood in 1-min fractions. Immunoreactive PTH (IPTH) concentrations were determined in quadruplicate, and the results were subjected to spectral analysis. In both the precava and postcava of normocalcemic dogs, there were regular oscillations in IPTH levels with a period of 12 min (range, 10–15 min) and a +/- 14% variation about the overall mean. Although significant two- to fourfold changes in IPTH levels still occurred during constant hypocalcemia, there was no significant rhythmicity. Significant cycles in IPTH concentration (mean 8.4-min period) were observed in thyroid venous effluent plasma during normocalcemia, confirming that the phenomenon represented episodic secretion that was not affected by pentobarbital anesthesia.

scholarly journals Determination of residues in chromogranin A-(16-40) required for inhibition of parathyroid hormone secretion.

Endocrinology ◽  
1996 ◽  
Vol 137 (7) ◽  
pp. 2918-2922 ◽  
Author(s):  
R H Angeletti ◽  
L Mints ◽  
C Aber ◽  
J Russell
Keyword(s):  
Parathyroid Hormone ◽  
Chromogranin A ◽  
Hormone Secretion

Weak Beam Imaging and Diffraction Studies of Stacking Faults in Silicon

1976 ◽  
Vol 34 ◽  
pp. 590-591
Author(s):  
T. Y. Tan ◽  
W. K. Tice
Keyword(s):  
Fast Neutron ◽  
Rapid Determination ◽  
Stacking Faults ◽  
Imaging Method ◽  
Large Reduction ◽  
Dislocation Loops ◽  
Fringe Spacing ◽  
Weak Beam ◽  
Kinematical Theory

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.

Determination of Myocardial FFA Elimination Rates by Functional Images of Uncorrected Half-Time Values

1984 ◽  
Vol 23 (06) ◽  
pp. 277-282 ◽  
Author(s):  
A. Van Lingen ◽  
G. Westera ◽  
M. van ◽  
W. Den Hollander ◽  
E. E. Van der Wall ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Regions Of Interest ◽  
Heptadecanoic Acid ◽  
Half Time ◽  
Alternative Method ◽  
Functional Images ◽  
Functional Image ◽  
Back Ground ◽  
Objective Determination

SummaryThis paper presents an alternative method of demarcating regions of in terest over the myocardium after ad ministration of 123I-heptadecanoic acid to patients with coronary artery disea se. In a matrix of 32 × 32 pixels the elimination rates of the radioactivity, which are not corrected for back ground activity, are visualized per pixel in a functional image. The func tional image showed areas in the myocardium with high values of uncorrected elimination rates. These areas corresponded with the tracer defects on the scintigram. Corrected elimination rates obtained from re gions of interest of functional images were comparable with those of scinti grams. Thus based on functional im ages of uncorrected elimination rates a reliable, objective determination of regions of interest over normal and abnormal myocardium can be made.

Methods for rapid determination of pecan (Carya illinoensis) seed viability

2017 ◽  
Vol 45 (2) ◽  
pp. 455-464
Author(s):  
T.T. Xue ◽  
J. Liu ◽  
Y.B. Shen ◽  
G.Q. Liu
Keyword(s):  
Rapid Determination ◽  
Seed Viability ◽  
Carya Illinoensis

PARATHYROID HORMONE SECRETION IN DISORDERS OF CALCIUM METABOLISM STUDIED BY MEANS OF EDTA

1974 ◽  
Vol 75 (2) ◽  
pp. 286-296 ◽  
Author(s):  
J. H. Lockefeer ◽  
W. H. L. Hackeng ◽  
J. C. Birkenhäger
Keyword(s):  
Parathyroid Hormone ◽  
Primary Hyperparathyroidism ◽  
Calcium Metabolism ◽  
Hormone Secretion ◽  
Parathyroid Tissue ◽  
Small Mass ◽  
Idiopathic Hypercalciuria ◽  
List Type ◽  
Immunoreactive Parathyroid Hormone ◽  
Pth Level

ABSTRACT In 22 of 28 cases of primary hyperparathyroidism (PHP) the rise in the serum immunoreactive parathyroid hormone (IRPTH or PTH) level observed in response to lowering of the serum calcium by EDTA, exceeded that obtained in 8 control subjects. In 5 of these 22 patients who were studied again after parathyroidectomy the supranormal response was abolished. Fifteen of these 22 hyper-responsive PHP patients had basal IRPTH levels not exceeding the highest level in the controls and that of other groups of patients investigated (idiopathic hypercalciuria, non-parathyroid hypercalcaemia, operated PHP). Fourteen of the 22 hyper-reactive patients with PHP did not show hypocalcaemia during the infusion of EDTA. The extent of the release of PTH elicited by EDTA in cases of PHP does not as yet allow a prediction of the amount of pathological parathyroid tissue present, although all the PHP patients showing a normal release of PTH had a relatively small mass of parathyroid tissue (up to about 1 g) subsequently removed. In 9 cases of nephrolithiasis (8 of whom had idiopathic hypercalciuria) and in 7 cases of non-parathyroid hypercalcaemia, a normal PTH release was found.

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