Use of a single 13C NMR resonance of glutamate for measuring oxygen consumption in tissue

1999 ◽  
Vol 277 (6) ◽  
pp. E1111-E1121 ◽  
Author(s):  
F. Mark H. Jeffrey ◽  
Alexander Reshetov ◽  
Charles J. Storey ◽  
Rui A. Carvalho ◽  
A. Dean Sherry ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Oxygen Consumption ◽  
Citric Acid ◽  
Magnetic Resonance ◽  
Citric Acid Cycle ◽  
Single Measurement ◽  
Acid Cycle ◽  
Nmr Data ◽  
Rat Hearts ◽  
C Nmr

A kinetic model of the citric acid cycle for calculating oxygen consumption from13C nuclear magnetic resonance (NMR) multiplet data has been developed. Measured oxygen consumption (MV˙o 2) was compared with MV˙o 2 predicted by the model with 13C NMR data obtained from rat hearts perfused with glucose and either [2-13C]acetate or [3-13C]pyruvate. The accuracy of MV˙o 2 measured from three subsets of NMR data was compared: glutamate C-4 and C-3 resonance areas; the doublet C4D34 (expressed as a fraction of C-4 area); and C-4 and C-3 areas plus several multiplets of C-2, C-3, and C-4. MV˙o 2 determined by set 2(C4D34 only) gave the same degree of accuracy as set 3(complete data); both were superior to set 1(C-4 and C-3 areas). Analysis of the latter suffers from the correlation between citric acid cycle flux and exchange between α-ketoglutarate and glutamate, resulting in greater error in estimating MV˙o 2. Analysis of C4D34 is less influenced by correlation between parameters, and this single measurement provides the best opportunity for a noninvasive measurement of oxygen consumption.

15N and 13C nuclear magnetic resonance of deoxydinucleotide monophosphates. II. Protonation of the homo dimers deoxycytidylyl-(3′,5′)-deoxycytidine, thymidylyl-(3′,5′)-thymidine, and deoxyadenylyl-(3′,5′)-deoxyadenosine in dimethyl sulfoxide

1990 ◽  
Vol 68 (11) ◽  
pp. 2033-2038 ◽  
Author(s):  
Giovanna Barbarella ◽  
Massimo Luigi Capobianco ◽  
Luisa Tondelli ◽  
Vitaliano Tugnoli
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Chemical Shift ◽  
Dimethyl Sulfoxide ◽  
Phosphate Group ◽  
Nmr Data ◽  
13C Nuclear Magnetic Resonance ◽  
C Nmr ◽  
Nuclear Magnetic

The preferential protonation sites of the homo dimers deoxycytidylyl-(3′,5′)-deoxycytidine, thymidylyl-(3′,5′)-thymidine, and deoxyadenylyl-(3′,5′)-deoxyadenosine were established by nitrogen-15 and carbon-13 NMR in dimethyl sulfoxide, in the presence of varying amounts of CF3COOH. The nitrogen-15 NMR data show that in d(CpC) the capability of the two N3 nitrogens to accept the proton is slightly different. In d(TpT) and d(ApA) the protonation of the phosphate group leads to significant variations of the chemical shift of the carbons adjacent to phosphorus. Keywords: deoxydinucleotides, protonation, 15N and 13C NMR.

13C-NMR spectroscopic evaluation of the citric acid cycle flux in conditions of high aspartate transaminase activity in glucose-perfused rat hearts

Biochimie ◽  
1998 ◽  
Vol 80 (12) ◽  
pp. 1013-1024 ◽  
Author(s):  
Son Tran-Dinh ◽  
Jacqueline A. Hoerter ◽  
Philippe Mateo ◽  
Franck Gyppaz ◽  
Martine Herve
Keyword(s):  
Citric Acid ◽  
13C Nmr ◽  
Citric Acid Cycle ◽  
Aspartate Transaminase ◽  
Transaminase Activity ◽  
Acid Cycle ◽  
Rat Hearts ◽  
Perfused Rat Hearts

scholarly journals Metabolism of Glycerol, Glucose, and Lactate in the Citric Acid Cycle Prior to Incorporation into Hepatic Acylglycerols

2013 ◽  
Vol 288 (20) ◽  
pp. 14488-14496 ◽  
Author(s):  
Eunsook S. Jin ◽  
A. Dean Sherry ◽  
Craig R. Malloy
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Hepatic Lipogenesis ◽  
Glycerol Backbone ◽  
Substantial Fraction ◽  
Acid Cycle ◽  
Glycerol Moiety ◽  
The Third ◽  
Relative Contribution ◽  
C Nmr

During hepatic lipogenesis, the glycerol backbone of acylglycerols originates from one of three sources: glucose, glycerol, or substrates passing through the citric acid cycle via glyceroneogenesis. The relative contribution of each substrate source to glycerol in rat liver acylglycerols was determined using 13C-enriched substrates and NMR. Animals received a fixed mixture of glucose, glycerol, and lactate; one group received [U-13C6]glucose, another received [U-13C3]glycerol, and the third received [U-13C3]lactate. After 3 h, the livers were harvested to extract fats, and the glycerol moiety from hydrolyzed acylglycerols was analyzed by 13C NMR. In either fed or fasted animals, glucose and glycerol provided the majority of the glycerol backbone carbons, whereas the contribution of lactate was small. In fed animals, glucose contributed >50% of the total newly synthesized glycerol backbone, and 35% of this contribution occurred after glucose had passed through the citric acid cycle. By comparison, the glycerol contribution was ∼40%, and of this, 17% of the exogenous glycerol passed first through the cycle. In fasted animals, exogenous glycerol became the major contributor to acylglycerols. The contribution from exogenous lactate did increase in fasted animals, but its overall contribution remained small. The contributions of glucose and glycerol that had passed through the citric acid cycle first increased in fasted animals from 35 to 71% for glucose and from 17 to 24% for glycerol. Thus, a substantial fraction from both substrate sources passed through the cycle prior to incorporation into the glycerol moiety of acylglycerols in the liver.

Carbon flux through citric acid cycle pathways in perfused heart by 13 C NMR spectroscopy

FEBS Letters ◽  
1987 ◽  
Vol 212 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Craig R. Malloy ◽  
A.Dean Sherry ◽  
F.Mark H. Jeffrey
Keyword(s):  
Citric Acid ◽  
Nmr Spectroscopy ◽  
Carbon Flux ◽  
Citric Acid Cycle ◽  
Perfused Heart ◽  
Acid Cycle ◽  
C Nmr

scholarly journals Probing the Origin of Acetyl-CoA and Oxaloacetate Entering the Citric Acid Cycle from the13C Labeling of Citrate Released by Perfused Rat Hearts

1997 ◽  
Vol 272 (42) ◽  
pp. 26117-26124 ◽  
Author(s):  
Blandine Comte ◽  
Geneviève Vincent ◽  
Bertrand Bouchard ◽  
Christine Des Rosiers
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Acetyl Coa ◽  
Acid Cycle ◽  
Rat Hearts ◽  
Perfused Rat Hearts

Propionyl-L-carnitine-mediated improvement in contractile function of rat hearts oxidizing acetoacetate

1995 ◽  
Vol 268 (1) ◽  
pp. H441-H447 ◽  
Author(s):  
R. R. Russell ◽  
J. I. Mommessin ◽  
H. Taegtmeyer
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Mechanical Performance ◽  
Ketone Bodies ◽  
Contractile Function ◽  
Sustained Improvement ◽  
Acid Cycle ◽  
Rat Hearts ◽  
Beta Hydroxybutyrate ◽  
Contractile Failure

Prior evidence has suggested that propionyl-L-carnitine improves function in ischemic hearts by providing carnitine for dissipation of acyl-CoA derivatives and propionate for enrichment of the citric acid cycle. Because contractile failure in hearts oxidizing ketone bodies is due to sequestration of free coenzyme A, which can be reversed by the addition of anaplerotic substrates that enrich the citric acid cycle, experiments were performed to determine whether the addition of propionyl-L-carnitine (2 mM) can improve performance in working rat hearts utilizing acetoacetate (7.5 mM). Whereas the addition of propionyl-L-carnitine to acetoacetate resulted in a sustained improvement in the work output of the heart, the addition of propionate (2 mM) or L-carnitine (2 mM) alone to acetoacetate had negligible effects on contractile function. Propionyl-L-carnitine increased the uptake of acetoacetate by 130%, whereas beta-hydroxybutyrate release was minimal and unchanged compared with other groups. These observations show that rates of acetoacetate oxidation are increased commensurate with increased contractile function. Tissue metabolite data indicate that the utilization of propionyl-L-carnitine did not lead to accumulation of citric acid cycle intermediates in the span from citrate to 2-oxoglutarate but to an increase in the tissue content of malate. The results show that addition of propionyl-L-carnitine in hearts oxidizing acetoacetate results in improved mechanical performance that is comparable to the mechanical performance of hearts perfused with glucose as the only substrate. This improvement is most likely conferred by anaplerosis, as suggested by enhanced rates of acetoacetate utilization and citric acid flux.

Carbon-13 nuclear magnetic resonance spectra of organic sulfur compounds. Substituent chemical shift (s.c.s.) effects in the 4-thiazoline-2-thione series

1976 ◽  
Vol 54 (10) ◽  
pp. 1660-1664 ◽  
Author(s):  
I. W. J. Still ◽  
N. Plavac ◽  
D. M. McKinnon ◽  
M. S. Chauhan
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Chemical Shift ◽  
Sulfur Compounds ◽  
Organic Sulfur ◽  
Organic Sulfur Compounds ◽  
Nmr Data ◽  
Nuclear Magnetic Resonance Spectra ◽  
C Nmr ◽  
Magnetic Resonance Spectra

13C nmr data have been obtained for a series of 4-thiazoline-2-thiones. Substituent chemical shift (s.c.s.) effects for methyl and phenyl substitution have been collated and are discussed in comparison with s.c.s. effects in other heterocyclic systems. Some attempt has also been made to compare our data with those reported previously for the thiazoles and for some thiones in the isothiazole series.

scholarly journals Configurational Study of Diastereoisomeric Royleanone Diterpenoids From Salvia concolor

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986265
Author(s):  
Mónica Díaz-Fernández ◽  
María I. Salazar ◽  
Pedro Joseph-Nathan ◽  
Eleuterio Burgueño-Tapia
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Chemical Shift ◽  
Absolute Configuration ◽  
Vibrational Circular Dichroism ◽  
Chemical Shift Assignments ◽  
Aerial Parts ◽  
Nmr Data ◽  
Nmr Chemical Shift Assignments ◽  
C Nmr

The known diterpenoids horminone (1) and taxoquinone (2) as a mixture, and pure 6,7-dehydroroyleanone (7) were isolated from the aerial parts of not yet studied Salvia concolor Lamb. Although 1 and 2 are known, the 1H Nuclear Magnetic Resonance (NMR) data of their acetyl derivatives 4 to 6 are only partially described. Moreover, the 13C NMR data assignments for 4 and 6 show some inconsistencies and reveal better agreement with those we obtained for diastereoisomeric 3 and 5. Since stereochemical aspects were in doubt, it was considered as mandatory to unambiguously determine the absolute configuration of 3 to 6 using vibrational circular dichroism spectroscopy, which then allowed the complete 1H and 13C NMR chemical shift assignments of 4 to 6.

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