A simple method for chronic cannulation of the portal vein in intact unrestrained rats

1988 ◽  
Vol 255 (2) ◽  
pp. G191-G193 ◽  
Author(s):  
A. Braillon ◽  
M. J. Brody
Keyword(s):  
Portal Vein ◽  
Direct Injection ◽  
New Method ◽  
Hypertensive Rats ◽  
Portal Vein Pressure ◽  
Simple Method ◽  
Blood Samples ◽  
Mesenteric Vein ◽  
Disease States ◽  
Chronic Cannulation

A simple new method is described for chronic cannulation of the portal vein in rats. A Silastic catheter (0.64 mm OD) with an internal metallic leader is directly inserted in the junction of the two small ileal veins. The catheter is advanced into the mesenteric vein to the portal vein. Then the leader is removed and the catheter tunneled out the back of the neck. This method allows for the measurement of portal vein pressure, direct injection of substances in the portal vein, or blood samples for at least 1 wk in intact unrestrained rats. Validation included the presence of elevated portal vein pressure in portal hypertensive rats compared with normal rats and the absence of clot development in the portal vein. This method should help provide a better understanding of hepatic physiology and pharmacology in normal and disease states.

Splanchnic vein closures

2019 ◽  
Vol 98 (6) ◽  
pp. 239-244
Keyword(s):  
Portal Vein ◽  
Surgical Approach ◽  
Medical Problem ◽  
Budd Chiari Syndrome ◽  
Extrahepatic Portal Vein Obstruction ◽  
Mesenteric Vein ◽  
Vein Thrombosis ◽  
Portal Vein Obstruction ◽  
Chiari Syndrome ◽  
Outflow System

Closures in the splanchnic venous system (SVS) represent a broad medical problem. Anatomically, individual or even multiple sections of SVS may be affected at the same time. Main sections of SVS include the venous liver outflow system, the portal vein, and the upper mesenteric vein and its basin. Thrombosis is clearly the predominant cause of closure. The closures can present as acute, subacute, chronic occult or chronic manifest. The main pathological and anatomical units are the Budd-Chiari syndrome (BCS), extrahepatic portal vein obstruction (EHPVO) and mesenteric vein thrombosis (MVT). Advanced laboratory, imaging and intervention methods substantially modify the approach to prevention, diagnosis and treatment; surgical approach also plays a role. The problem of SVS closures is interdisciplinary.

Capturing Protein Droplets: Label-free Visualization and Detection of Protein Liquid-Liquid Phase Separation with an Aggregation-Induced Emission Fluorogen

2021 ◽  
Author(s):  
Yan-Mei Li ◽  
Chu-Qiao Liang ◽  
Lin Wang ◽  
Yun-Yi Luo ◽  
Qian-Qian Li
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
New Method ◽  
Label Free ◽  
Simple Method ◽  
Aggregation Induced Emission ◽  
Liquid Liquid Phase Separation

We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of...

scholarly journals A simple new method for identifying performance characteristics associated with success in elite tennis

2018 ◽  
Vol 14 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Anna Fitzpatrick ◽  
Joseph A Stone ◽  
Simon Choppin ◽  
John Kelley
Keyword(s):  
Performance Analysis ◽  
Statistical Methods ◽  
Rank Order ◽  
Large Datasets ◽  
Performance Characteristics ◽  
New Method ◽  
Considerable Time ◽  
Simple Method ◽  
Suitable Alternative ◽  
Using Data

Performance analysis and identifying performance characteristics associated with success are of great importance to players and coaches in any sport. However, while large amounts of data are available within elite tennis, very few players employ an analyst or attempt to exploit the data to enhance their performance; this is partly attributable to the considerable time and complex techniques required to interpret these large datasets. Using data from the 2016 and 2017 French Open tournaments, we tested the agreement between the results of a simple new method for identifying important performance characteristics (the Percentage of matches in which the Winner Outscored the Loser, PWOL) and the results of two standard statistical methods to establish the validity of the simple method. Spearman’s rank-order correlations between the results of the three methods demonstrated excellent agreement, with all methods identifying the same three performance characteristics ( points won of 0–4 rally length, baseline points won and first serve points won) as strongly associated with success. Consequently, we propose that the PWOL method is valid for identifying performance characteristics associated with success in tennis, and is therefore a suitable alternative to more complex statistical methods, as it is simpler to calculate, interpret and contextualise.

Effect of short-term propionate infusion on feed intake and blood parameters in sheep

1997 ◽  
Vol 272 (6) ◽  
pp. E997-E1001 ◽  
Author(s):  
H. G. Leuvenink ◽  
E. J. Bleumer ◽  
L. J. Bongers ◽  
J. van Bruchem ◽  
D. van der Heide
Keyword(s):  
Portal Vein ◽  
Feed Intake ◽  
Blood Parameters ◽  
Feeding Pattern ◽  
Short Term ◽  
Mesenteric Vein ◽  
The Difference ◽  
High Doses

The hypothesis that propionate is a short-term feed intake-regulating agent was studied. Mature wether sheep were infused over 20 min with Na propionate into the mesenteric vein, while feed intake and feeding pattern were monitored over 1.5 h. Feed intake was reduced by infusions at 2 mmol/min, which were associated with marked increases in jugular as well as portal concentrations of insulin, glucose, and propionate. In a second experiment, animals were infused with 2 mmol/min Na propionate into the portal vein. No decrease in feed intake was observed, although there were similar increases in insulin, glucose, and propionate as found in mesenteric vein-infused animals. It is concluded that mesenteric propionate in high doses acts as a satiety factor. Possible explanations for the difference between site of infusion may be a different distribution of the infusate over the liver and/or the presence of propionate-sensitive receptors in the mesenteric/portal vein region. It seems unlikely that insulin concentrations are involved in inducing satiety in propionate-infused animals.

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