Chromatographic separation of pepsins from human gastric juice

1964 ◽  
Vol 207 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Peptic Ulcer ◽  
Pernicious Anemia ◽  
Gastric Juice ◽  
Chromatographic Separation ◽  
Healthy Individuals ◽  
Chromatographic Mobility ◽  
Pepsinogen I ◽  
Human Gastric Juice ◽  
Diethylaminoethyl Cellulose

Acid gastric juices have been fractionated on DEAE (diethylaminoethyl)-cellulose to yield three pepsin fractions, which have the same chromatographic mobility as the three pepsin fractions previously described in acidified whole gastric mucosal extracts. The acid gastric juices included specimens obtained from healthy individuals and from patients with peptic ulcer and gastritis. Gastric juice from one patient with pernicious anemia was shown to contain only pepsinogen I. A satisfactory chromatographic separation of pepsins is possible from as little as 4 ml of gastric juice.

scholarly journals Immunologic Studies in Pernicious Anemia

Blood ◽  
1969 ◽  
Vol 34 (1) ◽  
pp. 63-71 ◽  
Author(s):  
CHIAKI TAI ◽  
JAMES E. MCGUIGAN
Keyword(s):  
Pernicious Anemia ◽  
Gastric Juice ◽  
Cellular Immunity ◽  
Tritiated Thymidine ◽  
Intrinsic Factor ◽  
Lymphocyte Transformation ◽  
Gastric Parietal Cell ◽  
Human Gastric Juice ◽  
Blocking Antibodies

Abstract Experiments were directed to the investigation of evidence of a possible role of cellular immunity in patients with pernicious anemia. Lymphocyte-rich peripheral leucocyte preparations from 29 patients with pernicious anemia were cultured in the presence of a variety of preparations containing potential antigens: these included human gastric juice, human intrinsic factor (IF) preparations, and human gastric mucosal homogenates. Lymphocyte transformation was determined by measurement of the uptake of tritiated thymidine into DNA. Lymphocyte transformation occurred when lymphocytes from a portion of the patients with pernicious anemia (3.4 per cent to 37.5 per cent) were cultured in the presence of these antigen preparations. Lymphocyte transformation was noted in none of the patients whose sera contained blocking antibodies to intrinsic factor. There was no correlation between the presence or absence of lymphocyte transformation and the presence or absence of serum antibodies to the gastric parietal cell cytoplasmic antigen. These data on lymphocyte transformation permit the consideration that cellular immunity may participate in the pathogenesis of pernicious anemia.

Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa

1963 ◽  
Vol 205 (6) ◽  
pp. 1106-1112 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Duodenal Mucosa ◽  
Human Gastric Mucosa ◽  
Chromatographic Mobility ◽  
Pepsinogen I ◽  
Fundic Mucosa ◽  
Inhibitor Complex ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii ◽  
Pepsin Inhibitor

Extracts of acidified human gastric fundic mucosa have been fractionated on diethylaminoethyl cellulose to yield three pepsin fractions, which have, in order of their elution, been attributed to pepsin I, pepsin II A, and a mixture of pepsin II B and pepsin III. Acidification of previously purified pepsinogen I and pepsinogen III yields pepsin I and pepsin III, respectively. Acidification of previously purified pepsinogen II yields two distinct pepsin fractions, pepsin II A and pepsin II B. Pepsin II B and pepsin III have the same chromatographic mobility on diethylaminoethyl cellulose, but are probably not identical. Pyloric and duodenal mucosa yield only pepsin I upon acidification. Human pepsin-pepsin inhibitor complex is demonstrable as a chromatographic fraction distinct from pepsins upon fractionation of acidified dilute pepsinogen solutions with diethylaminoethyl cellulose equilibrated with acetate buffer pH 5.3.

The role of microcirculation disorders in the pathogenesis of peptic ulcer disease

1982 ◽  
Vol 63 (3) ◽  
pp. 21-23
Author(s):  
V. F. Bogoyavlenskiy ◽  
A. G. Oparin ◽  
R. M. Gazizov ◽  
T. I. Rupasova
Keyword(s):  
Duodenal Ulcer ◽  
Peptic Ulcer ◽  
Gastric Ulcer ◽  
Peptic Ulcer Disease ◽  
Gastric Juice ◽  
The State ◽  
Healthy Individuals ◽  
Ulcer Disease

In 60 healthy individuals and 85 patients with gastric ulcer and duodenal ulcer, the state of microcirculation of the conjunctiva of the eye and the level of glycoproteins in the gastric juice were studied. It has been shown that one of the reasons leading to a decrease in the level of mucins in gastric juice may be mucosal hypoxia caused by impaired microcirculation.

Digestive Action of Human Gastric Juice.

1956 ◽  
Vol 92 (2) ◽  
pp. 237-240 ◽  
Author(s):  
J. F. Perry ◽  
E. G. Yonehiro ◽  
P. M. Ya ◽  
H. D. Root ◽  
O. H. Wangensteen
Keyword(s):  
Gastric Juice ◽  
Human Gastric Juice
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