Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa

1963 ◽  
Vol 205 (6) ◽  
pp. 1106-1112 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Duodenal Mucosa ◽  
Human Gastric Mucosa ◽  
Chromatographic Mobility ◽  
Pepsinogen I ◽  
Fundic Mucosa ◽  
Inhibitor Complex ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii ◽  
Pepsin Inhibitor

Extracts of acidified human gastric fundic mucosa have been fractionated on diethylaminoethyl cellulose to yield three pepsin fractions, which have, in order of their elution, been attributed to pepsin I, pepsin II A, and a mixture of pepsin II B and pepsin III. Acidification of previously purified pepsinogen I and pepsinogen III yields pepsin I and pepsin III, respectively. Acidification of previously purified pepsinogen II yields two distinct pepsin fractions, pepsin II A and pepsin II B. Pepsin II B and pepsin III have the same chromatographic mobility on diethylaminoethyl cellulose, but are probably not identical. Pyloric and duodenal mucosa yield only pepsin I upon acidification. Human pepsin-pepsin inhibitor complex is demonstrable as a chromatographic fraction distinct from pepsins upon fractionation of acidified dilute pepsinogen solutions with diethylaminoethyl cellulose equilibrated with acetate buffer pH 5.3.

Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from human gastric mucosa

1963 ◽  
Vol 205 (6) ◽  
pp. 1099-1105 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Proteolytic Enzymes ◽  
Low Ph ◽  
Human Gastric Mucosa ◽  
Pepsinogen I ◽  
Fundic Mucosa ◽  
Proximal Duodenum ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii

Extracts of human gastric mucosa have been fractionated on diethylaminoethyl cellulose to yield three precursors of proteolytic enzymes active at low pH which have been called pepsinogen I, pepsinogen II, and pepsinogen III. Pepsinogen I has been found in the mucosa from all parts of the stomach examined as well as in the proximal duodenum. Pepsinogen II and pepsinogen III were present in fundic mucosa only.

scholarly journals Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa

1964 ◽  
Vol 207 (6) ◽  
pp. 1451-1451
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Companion Paper ◽  
Human Gastric Mucosa ◽  
Pepsinogen I ◽  
Volume Table ◽  
Pepsinogen Ii

Page 1106: Max J. Seijffers, Harry L. Segal, and Leon L. Miller. "Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa." Title in abstract, in issue table of contents, and in volume table of contents printed in error as title of companion paper which begins on page 1099. Should read the same as above title.

Separation of pepsinogen II and pepsinogen III from human urine

1964 ◽  
Vol 206 (5) ◽  
pp. 1106-1110 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Proteolytic Activity ◽  
Human Urine ◽  
Chromatographic Behavior ◽  
Pepsinogen I ◽  
Qualitative And Quantitative ◽  
Quantitative Correlation ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii ◽  
Urine Specimens

Human urine has been fractionated on diethylaminoethyl cellulose to yield two pepsinogens. They have been called pepsinogen II and pepsinogen III on the basis of chromatographic behavior identical to that of fundic mucosal pepsinogens II and III. Pepsinogen I has been found absent from 23 urine specimens of 12 individuals. Results suggest a gross qualitative and quantitative correlation between pepsinogen II-pepsinogen III ratio (in terms of total proteolytic activity) in urine and gastric mucosa.

Chromatographic separation of pepsins from human gastric juice

1964 ◽  
Vol 207 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Peptic Ulcer ◽  
Pernicious Anemia ◽  
Gastric Juice ◽  
Chromatographic Separation ◽  
Healthy Individuals ◽  
Chromatographic Mobility ◽  
Pepsinogen I ◽  
Human Gastric Juice ◽  
Diethylaminoethyl Cellulose

Acid gastric juices have been fractionated on DEAE (diethylaminoethyl)-cellulose to yield three pepsin fractions, which have the same chromatographic mobility as the three pepsin fractions previously described in acidified whole gastric mucosal extracts. The acid gastric juices included specimens obtained from healthy individuals and from patients with peptic ulcer and gastritis. Gastric juice from one patient with pernicious anemia was shown to contain only pepsinogen I. A satisfactory chromatographic separation of pepsins is possible from as little as 4 ml of gastric juice.

scholarly journals Non-invasive laboratory markers assessing efficacy of Helicobacter pylori eradication therapy

2019 ◽  
Vol 9 (3-4) ◽  
pp. 523-530
Author(s):  
L. B. Drygina ◽  
V. N. Ellenidi ◽  
N. A. Bardysheva ◽  
M. M. Bogoslovskiy
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Eradication Therapy ◽  
Helicobacter Pylori Infection ◽  
Igg Antibodies ◽  
Ki 67 ◽  
Helicobacter Pylori Eradication ◽  
Pepsinogen I ◽  
Non Invasive ◽  
Pepsinogen Ii

Effective eradication of Helicobacter pylori infection is an important means to reduce the risk of precancerous changes in the gastric mucosa and prevention of gastric cancer. A search for non-invasive diagnostic tools for Helicobacter pylori infection, evaluation of the effectiveness of eradication remains of high importance.The aim of the study was to assess an informative value of detecting pepsinogen I and II as well as serum antibodies to Helicobacter pylori while assessing an efficacy of treated chronic Helicobacter gastritis and identifying preneoplastic changes in the stomach mucosa. There enrolled 113 male patients with chronic gastritis aged 41 to 76, average age- (56.7±0.7) years. Examination of patients was carried out at admission to the clinic, as well as at 2 and 12 months after administering a standard eradication therapy. It was found that Helicobacter pylori infection was detected in 101 (89.4%) patients. Groups of patients with effective eradication therapy were noted. A time-dependent level of antibodies to Helicobacter pylori, as well as measured concentration of pepsinogen I and II after the onset of eradication treatment were determined. Which were analyzed in connection with the results of histology examination of gastric mucosa biopsy specimens and expression of oncoproteins Ki-67, Bcl-2, c-erbB-2, p16 in the gastric mucosa depending on efficacy of eradication therapy. It is shown that effective eradication therapy was characterized by significantly decreased serum level of IgG antibodies to Helicobacter pylori 2 months after the onset of treatment. Moreover, a significantly decreased pepsinogen II and serum IgG antibodies to Helicobacter pylori during eradication therapy were accompanied by a significant decrease in Ki-67 expression in the gastric epithelium. Decreased concentration of pepsinogen II within the first year after Helicobacter pylori eradication therapy was due to a greater decrease in activity of inflammatory changes in the gastric mucosa than to dynamic changes in gastric atrophy and metaplasia. An inverse relation between the serum level of pepsinogen I and atrophy as well as intestinal metaplasia within the gastric mucosa were found.

Helicobacter pylori infection: place of serological and cultural diagnostics in clinical guidelines

2021 ◽  
Vol 66 (8) ◽  
pp. 496-501
Author(s):  
L. A. Kornoukhova ◽  
V. L. Emanuel ◽  
N. L. Denisov ◽  
E. L. Nikonov
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Blood Serum ◽  
Clinical Guidelines ◽  
Reference Intervals ◽  
Healthy People ◽  
Helicobacter Pylori Infection ◽  
Pepsinogen I ◽  
H Pylori ◽  
Pepsinogen Ii

The purpose of this work was to familiarize doctors with the methods and significance of serological and cultural diagnostics of H. pylori infection on the example of the test for diagnosing the state of the gastric mucosa «Gastropanel». Blood serum tests were performed for 1057 patients and 122 healthy people aged 18-64 years: pepsinogen I (PG I), pepsinogen II (PG II), gastrin-17 (G-17), basal/stimulated), antibodies (IgGHp) to H. pylori (Biohit Oyj, Finland). The medians of the studied group indicators did not exceed the reference intervals. 398 (34%) patients have negative H. pylori status (IgGHp-). 275 (26%) patients with serum PG I≤70 mcg/ml were identified. The ratio of PG I/II≤3 in 84 (8%), 36 of them (43% of the group PG I/II≤3) - IgGHp-.

EXPERIENCE OF USING THE TEST SYSTEM «GASTROPANEL» IN THE PREVENTIVE MEDICAL EXAMINATION OF WORKFORCE IN JSC RZD

2021 ◽  
Author(s):  
A.O. Devyatko ◽  
◽  
T.A. Noskova ◽  
N.V. Shevchenko
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Enzyme Immunoassay ◽  
Test System ◽  
Medical Examination ◽  
Pepsinogen I ◽  
Non Invasive ◽  
H Pylori ◽  
Pepsinogen Ii

Abstract: Non-invasive diagnosis of the gastric mucosa was carried out in 181 people in the preventive medical examination of workforce in JSC RZD. A set of diagnostics from Vector-Best (Novosibirsk, Russia) was used for enzyme immunoassay of pepsinogen I, pepsinogen II, and immunoglobulins for Helicobacter pylori. The prevalence of H. pylori infection in more than half of the employees, the presence of deviations of biomarkers from the norm in more than one in three employees were revealed. Laboratory signs of atrophy were detected eight times less frequently than signs of inflammation of the gastric mucosa, but when infected with H. pylori and increased with age.

scholarly journals Ultrastructural Immunogold Localization of Osteopontin in Human Gastric Mucosa

1997 ◽  
Vol 45 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Qu Hong ◽  
Lawrence F. Brown ◽  
Harold F. Dvorak ◽  
Ann M. Dvorak
Keyword(s):  
Gastric Mucosa ◽  
Lamina Propria ◽  
Secretory Granules ◽  
Mucous Cell ◽  
Human Gastric Mucosa ◽  
Epithelial Layer ◽  
Immunogold Localization ◽  
Epithelial Surface ◽  
Chief Cells ◽  
Pepsinogen Ii

We performed ultrastructural immunogold localization of osteopontin in the mucosa of human stomach. This adhesive glycoprotein was present in mucous and chief cells of the epithelial layer and in macrophages in the lamina propria. Parietal and endocrine cells of the epithelial layer and mast cells and plasma cells in the lamina propria did not contain osteopontin, serving as internal negative controls. Subcellular localizations of osteopontin included secretory granules and synthetic organelles in mucous and chief cells and phagolysosomes in macrophages. Extracellular concentrations of osteopontin were present in the glycocalyx and in an electron-lucent band between epithelial surface cells and the gastric lumen. Paracellular edema between the epithelium of the same cells was devoid of osteopontin. Immunogold localization of pepsinogen II was done to identify cells with mixed granule populations and contents of multicompartmental secretory granules. These studies revealed mucous cell granules and chief cell granules, each containing compartmentalized storage products, which included osteopontin and mucigen in mucous cells and osteopontin and pepsinogen II in chief cells. Cytochemical controls for the immunogold localizations were negative. The subcellular distribution of osteopontin in human gastric mucosa suggests possible roles for this glycoprotein in barrier function, host defense, and/or secretion.

H. pylori down-regulates TRAIL (DR4)-receptors in the human gastric mucosa, a possible mechanism for chronic persistence

2001 ◽  
Vol 120 (5) ◽  
pp. A81-A81
Author(s):  
B NEU ◽  
R RAD ◽  
M NEUHOFER ◽  
C TRAUTWEIN ◽  
M GERHARD ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Human Gastric Mucosa ◽  
H Pylori
Sign in / Sign up

Export Citation Format

Share Document