Organ blood flow in Fischer-344 rats bearing MCA-induced sarcoma

1991 ◽  
Vol 71 (5) ◽  
pp. 1674-1678 ◽  
Author(s):  
P. A. Van Leeuwen ◽  
J. R. Bading ◽  
N. A. Vydelingum ◽  
R. N. Younes ◽  
P. de Rooij ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Organ Blood Flow ◽  
Fischer 344 Rats ◽  
Venous Flow ◽  
Fischer 344 ◽  
Abdominal Organs ◽  
Hindlimb Muscle ◽  
Distribution Of Cardiac Output ◽  
Host Tissues

Although blood flow is central to systemic metabolism, little is known about the effect of tumor on the perfusion of host tissues. This study evaluated the effects of a methylcholanthrene-induced sarcoma on blood flow to intra-abdominal organs and skeletal muscle of Fischer-344 rats anesthetized with pentobarbital sodium. Animals were studied by aortic injection of radiolabeled microspheres when the tumors reached 20% of body weight. Total-organ arterial flows in spleen, liver, small intestine, and pancreas were each increased to 50–150% in tumor bearers relative to controls (P less than 0.05). Portal venous flow and flow per gram to hindlimb muscle were 60 +/- 20 and 300 +/- 100% greater, respectively, in tumor-bearing animals (P less than 0.005). This study shows that tumor growth can be associated with large changes in organ flow and distribution of cardiac output. The increase in skeletal muscle flow in the tumor bearers, which lost normal tissue weight relative to pair-fed controls (P less than 0.05), is in marked contrast to decreased muscle flow previously observed in simple starvation.

scholarly journals Adrenergic stimulated skeletal muscle glycogenolysis in perfused hindlimbs of young and old male Fischer 344 rats

1994 ◽  
Vol 266 (3) ◽  
pp. R749-R755
Author(s):  
L. M. Larkin ◽  
B. A. Horwitz ◽  
K. C. Eiffert ◽  
R. B. McDonald
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Fischer 344 Rats ◽  
Fischer 344 ◽  
Hindlimb Muscle ◽  
Glycogen Utilization ◽  
Sciatic Nerve Stimulation ◽  
Glycogen Stores ◽  
F344 Rats ◽  
Krebs Henseleit Buffer

Epinephrine (Epi)- and forskolin (FSK)-stimulated glycogenolysis of skeletal muscle was evaluated in perfused hindlimb isolated from male Fischer 344 (F344) rats, ages 6, 12, and 26 mo. Muscle glycogen stores were reduced by sciatic nerve stimulation and replenished by infusing 10 mM glucose, 500 microU insulin, and 5 microCi [14C]glucose via a left carotid artery cannula. Then the hindlimb was perfused with a modified Krebs-Henseleit buffer (pH 7.4). At minute 20 of the perfusion, Epi [0.0 (perfusate), 0.25, 0.50, or 0.75 microM] or 40 microM FSK were infused for 10 min. Radioactivity (14C) in the effluent perfusate was collected every 60 s during a 20-min preinfusion, a 10-min Epi infusion, and a 20-min postinfusion period and was used to determine the rate of muscle glycogen utilization. Total 14C release increased with Epi and 40 microM FSK. However, the pattern of release did not differ significantly with age. In general, the fraction of the perfusate released as 14CO2 increased in the presence of FSK and Epi but did not significantly differ with age. [14C]lactate released in response to Epi increased in the 6-mo-old group, remained unchanged in the 12-mo-old group, and decreased in the 26-mo-old group compared with 0.0 Epi (perfusate) values. It appears that stimulation of skeletal muscle glycogenolysis via adrenergic receptor or postreceptor/adenosine 3',5'-cyclic monophosphate-mediated mechanisms is unaffected by age. However, the utilization of carbohydrate by isolated hindlimb muscle is altered in the aging rat, resulting in a more oxidative metabolism.

Effects of aging on cardiac output, regional blood flow, and body composition in Fischer-344 rats

1998 ◽  
Vol 85 (5) ◽  
pp. 1813-1822 ◽  
Author(s):  
Michael D. Delp ◽  
Marina V. Evans ◽  
Changping Duan
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Cardiac Output ◽  
Prostate Gland ◽  
Aged Rats ◽  
Adult Rats ◽  
Juvenile Rats ◽  
Fischer 344 ◽  
Brain And Spinal Cord ◽  
Distribution Of Cardiac Output

The purpose of this study was to determine the effects of maturation and aging on cardiac output, the distribution of cardiac output, tissue blood flow (determined by using the radioactive-microsphere technique), and body composition in conscious juvenile (2-mo-old), adult (6-mo-old), and aged (24-mo-old) male Fischer-344 rats. Cardiac output was lower in juvenile rats (51 ± 4 ml/min) than in adult (106 ± 5 ml/min) or aged (119 ± 10 ml/min) rats, but cardiac index was not different among groups. The proportion of cardiac output going to most tissues did not change with increasing age. However, the fraction of cardiac output to brain and spinal cord tissue and to skeletal muscle was greater in juvenile rats than that in the two adult groups. In addition, aged rats had a greater percent cardiac output to adipose tissue and a lower percent cardiac output to cutaneous and reproductive tissues than that in juvenile and adult rats. Differences in age also had little effect on mass-specific perfusion rates in most tissues. However, juvenile rats had lower flows to the pancreas, gastrointestinal tract, thyroid and parathyroid glands, and kidneys than did adult rats, and aged rats had lower flows to the white portion of rectus femoris muscle, spleen, thyroid and parathyroid glands, and prostate gland than did adult rats. Body mass of juvenile rats was composed of a lower percent adipose mass and a greater fraction of brain and spinal cord, heart, kidney, liver, and skeletal muscle than that of the adult and aged animals. Relative to the young adult rats, the body mass of aged animals had a greater percent adipose tissue mass and a lower percent skeletal muscle and skin mass. These data demonstrate that maturation and aging have a significant effect on the distribution of cardiac output but relatively little influence on mass-specific tissue perfusion rates in conscious rats. The old-age-related alterations in cardiac output distribution to adipose and cutaneous tissues appear to be associated with the increases in percent body fat and the decreases in the fraction of skin mass, respectively, whereas the decrease in the portion of cardiac output directed to reproductive tissue of aged rats appears to be related to a decrease in mass-specific blood flow to the prostate gland.

Effects of the Interaction between Carbogen and Nicotinamide on R3230 Ac Tumor Blood Flow in Fischer 344 Rats

2001 ◽  
Vol 155 (5) ◽  
pp. 724-733 ◽  
Author(s):  
Rod D. Braun ◽  
Jennifer L. Lanzen ◽  
Joe A. Turnage ◽  
Gary Rosner ◽  
Mark W. Dewhirst
Keyword(s):  
Blood Flow ◽  
Fischer 344 Rats ◽  
Tumor Blood Flow ◽  
Fischer 344

Reduced lipoprotein lipase activity in postural skeletal muscle during aging

2001 ◽  
Vol 91 (2) ◽  
pp. 687-692 ◽  
Author(s):  
Lionel Bey ◽  
Enas Areiqat ◽  
Andrea Sano ◽  
Marc T. Hamilton
Keyword(s):  
Skeletal Muscle ◽  
Lipoprotein Lipase ◽  
Soleus Muscle ◽  
Tibialis Anterior ◽  
Weight Bearing ◽  
Fischer 344 Rats ◽  
Protein Mass ◽  
Fischer 344 ◽  
Mrna Concentration ◽  
Postural Muscle

Lipoprotein lipase (LPL) is a key enzyme for fatty acid and lipoprotein metabolism in muscle. However, the effect of aging on LPL regulation in skeletal muscle is unknown. We report the effect of aging on LPL regulation in the soleus (red oxidative postural) muscle and the tibialis anterior (white glycolytic non-weight-bearing) muscle in 4- and 24-mo-old Fischer 344 rats and 18- and 31-mo-old Fischer 344 × Brown-Norway F1 (F-344 × BN F1) rats. Total and heparin-releasable LPL (HR-LPL) activities were decreased 38% ( P< 0.01) and 52% ( P < 0.05), respectively, in the soleus muscle of the older Fischer 344 rats. There was a 32% reduction ( P < 0.05) of total LPL protein mass in the soleus muscle with aging. The results were confirmed in another strain. A decrease of total LPL activity (−50%, P < 0.05) was also found in the soleus muscle between 18- and 31-mo-old F-344 × BN F1 rats. LPL mRNA concentration in the soleus muscle was not different between ages. Total LPL protein mass was reduced by 46% ( P < 0.05) in the soleus muscle of the 31-mo-old F-344 × BN F1 rats. In the tibialis anterior muscle, neither LPL activity nor mRNA concentration was affected by age in either strain. In conclusion, LPL regulation in a non-weight-bearing muscle was not affected by aging. However, there was a pronounced reduction in LPL activity and LPL protein mass in postural muscle with aging.

Effect of vasopressors on organ blood flow during endotoxin shock in pigs

1987 ◽  
Vol 252 (2) ◽  
pp. H291-H300 ◽  
Author(s):  
M. J. Breslow ◽  
C. F. Miller ◽  
S. D. Parker ◽  
A. T. Walman ◽  
R. J. Traystman
Keyword(s):  
Blood Pressure ◽  
Skeletal Muscle ◽  
Blood Flow ◽  
Left Ventricle ◽  
Arterial Blood Pressure ◽  
Endotoxin Shock ◽  
Organ Blood Flow ◽  
Shock Model ◽  
Blood Pressure Elevation ◽  
Arterial Blood

A volume-resuscitated porcine endotoxin shock model was used to evaluate the effect on organ blood flow of increasing systemic arterial blood pressure with vasopressors. Administration of 0.05–0.2 mg/kg of Escherichia coli endotoxin (E) reduced mean arterial blood pressure (MAP) to 50 mmHg, decreased systemic vascular resistance to 50% of control, and did not change cardiac output or heart rate. Blood flow to brain, kidney, spleen, and skeletal muscle was reduced during endotoxin shock, but blood flow to left ventricle, small and large intestine, and stomach remained at pre-endotoxin levels throughout the study period. Four groups of animals were used to evaluate the effect of vasopressor therapy. A control group received E and no vasopressor, whereas the other three groups received either norepinephrine, dopamine, or phenylephrine. Vasopressors were administered starting 60 min after E exposure, and the dose of each was titrated to increase MAP to 75 mmHg. Despite the increase in MAP, brain blood flow did not increase in any group. Norepinephrine alone increased blood flow to the left ventricle. Kidney, splanchnic, and skeletal muscle blood flow did not change with vasopressor administration. The dose of norepinephrine required to increase MAP by 20–25 mmHg during E shock was 30 times the dose required for a similar increase in MAP in animals not receiving E. We conclude that hypotension in the fluid resuscitated porcine E shock model is primarily the result of peripheral vasodilatation, that the vascular response to vasoconstrictors in this model is markedly attenuated following E administration, that blood pressure elevation with norepinephrine, dopamine, and phenylephrine neither decreases blood flow to any organ nor increases blood flow to organs with reduced flow, and that norepinephrine, dopamine, and phenylephrine affect regional blood flow similarly in this model.

Effect of serotonin on cardiac output and organ blood flow of rats

1963 ◽  
Vol 204 (2) ◽  
pp. 301-303 ◽  
Author(s):  
L. Takács ◽  
V. Vajda
Keyword(s):  
Blood Pressure ◽  
Skeletal Muscle ◽  
Blood Flow ◽  
Cardiac Output ◽  
Intravenous Infusion ◽  
Organ Distribution ◽  
Organ Blood Flow ◽  
Cutaneous Blood Flow ◽  
Pulmonary Parenchyma ◽  
Cutaneous Blood

The effects of intraperitoneal and intravenous administration of serotonin on cardiac output, blood pressure, and organ distribution of blood flow (Rb86) were studied in the rat. Fifteen to thirty minutes after intraperitoneal injection (10 mg/kg) cardiac output was unchanged, while blood pressure was significantly reduced. Increase in blood flow was noted in the myocardium, pulmonary parenchyma and "carcass" (skeletal muscle, bone, CNS), with decrease in the kidney and the skin. Splanchnic blood flow was unchanged. Conversely, intravenous infusion of serotonin produced an increase of cardiac output, blood pressure, and cutaneous blood flow.

Cardiac output and organ blood flow in warm- and cold-acclimated rats exposed to cold

1968 ◽  
Vol 46 (4) ◽  
pp. 653-659 ◽  
Author(s):  
L. Jansky ◽  
J. S. Hart
Keyword(s):  
Blood Flow ◽  
Cardiac Output ◽  
Skeletal Muscles ◽  
Organ Blood Flow ◽  
Internal Organs ◽  
Exposure To Cold ◽  
Brown Adipose ◽  
Production Increase ◽  
Distribution Of Cardiac Output ◽  
Fractional Distribution

Cold acclimation increased the cardiac output of unanesthetized rats when measured at 30 °C. After exposure to 9 °C for 70 min cardiac output further increased by 46% in both warm- and cold-acclimated rats. From the changes in the fractional distribution of cardiac output after cold exposure it was shown that the blood flow increased significantly in muscular organs (heart, diaphragm, skeletal muscles) and in the adrenals of warm-acclimated rats. In cold-acclimated rats the blood flow to the brown and white adipose tissues, pancreas, kidney, intestine, liver, and other internal organs was also increased in a cold environment, and accounted for 65% of the increase in blood flow during exposure to cold compared with only 36% in warm-acclimated rats. It is estimated that the extramuscular thermogenesis can account for a greater proportion of the total nonshivering thermogenesis in cold-acclimated rats. The contribution of brown adipose tissue is estimated not to exceed about 6% of the total heat production increase in cold-acclimated rats during exposure to cold.

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