Dynamic planar scintigraphy for the rapid kinetic measurement of myocardial 123I-MIBG turnover can identify Lewy body disease

Background Using two static scans for 123I-meta-iodobenzyl-guanidine (123I-MIBG) myocardial scintigraphy ignores the dynamic response from vesicular trapping in nerve terminals. Moreover, the long pause between scans is impractical for patients with Lewy body diseases (LBDs). Here, we optimized indices that capture norepinephrine kinetics, tested their diagnostic performance, and determined the differences in 123I-MIBG performance among disease groups. Methods We developed a new 30-min protocol for 123I-MIBG dynamic planar imaging for suspected LBD patients. Pharmacokinetic modelling of time-activity curves (TACs) was used to calculate three new indices: unidirectional uptake of 123I-MIBG to vesicular trapping (iUp), rate of myocardial 123I-MIBG loss (iLoss), and non-specific fractional distribution of 123I-MIBG in the interstitial space. We compared the performance of the new and existing indices with regard to discrimination of patients with or without LBDs. Subgroup analysis was performed to examine differences in 123I-MIBG turnover between patients in a dementia with Lewy bodies (DLB) group and two Parkinson’s disease (PD) groups, one with and the other without REM sleep behaviour disorder (RBD). Results iLoss was highly discriminative, particularly for patients with low myocardial 123I-MIBG trapping, and the new indices outperformed existing ones. ROC analysis revealed that the AUC of iLoss (0.903) was significantly higher than that of early HMR (0.863), while comparable to that of delayed HMR (0.892). The RBD-positive PD group and the DLB group had higher turnover rates than the RBD-negative PD group, indicating a potential association between prognosis and iLoss. Conclusion 123I-MIBG turnover can be quantified in 30 min using a three-parameter model based on 123I-MIBG TACs. The discriminatory performance of the new model-based indices might help explain the neurotoxicity or neurodegeneration that occurs in LBD patients.

Proteinogram of blood of animals in a state of hypobiosis during e.coli infection

The features of fractional distribution of plasma proteins in rats with Escherichia coli at different stages of infection are established. It was studied the effect of hypobiosis on the proteinogramma under and after artificial sleep too. Artificial hypobiosis is important for therapeutic use at bacterial infection and proteinogramma is informatively significant as a diagnostic element of the functional changes provoked by a hypobiosis. We used the nephelometric method. Experiments were performed on young rats with Escherichia coli, in a state of hypobiosis and one day after leaving it. It was established the infection provoked changes in plasma proteins such as increasing globulins and decreasing albumin at the beginning of the disease and the opposite trend during the period of total depletion. The introduction of sick animals into a state of hypobiosis causes an increase in nonspecific resistance of the organism level due to an increase in the content of γ-globulin fraction and a decrease in the level of albumin, which increases the survival of animals after removal from hypobiosis. Indicators of the proteinogram of blood of animals that were introduced into the state of hypobiosis at the initial stage of the disease and the stage of progressive symptoms return to control values on the first day after the elimination of hypobiosis factors. Further research should be aimed at a comprehensive study of functional changes in the body according to the proposed model.

PSIX-15 Intake of corn stover plant parts by feedlot beef steers

Corn stover supports feedlot operations in intensive corn producing regions. A single-pass corn grain and stover harvest system was developed to increase efficiency of field operations and capture different anatomical fractions than are typically harvested with conventional corn stover. The objectives were to feed beef steers diets that included a roughage component consisting of harvested corn residue in chopped form from conventional corn stover bales (CST) or single-pass bales (SPB). Whole-plant corn silage (WPCS) served as a control. Steers (n = 90, 5 pens/treatment) were fed during Grow (84 d) and Finish (66 d) phases to assess consumption of corn plant botanical fractions and calculate net energy values of the stover feeds. Cattle consumed a larger proportion of stover as cob (P < 0.001) and less as stalk (P = 0.001) when stover was offered as SPB rather than CST. These differences are consistent with the fractional distribution of botanical components offered. During the Grow phase, cattle fed WPCS had greater (P = 0.018) daily gains (1.27 kg d-1) than cattle fed the SPB (1.14 kg d-1) and CST (1.08 kg d-1), and were more efficient than CST cattle. Steers sorted corn stover during both phases and consumed 52.5% of corn stover offered. SPB cob intake was 70% greater than CST cob intake (P < 0.01) indicating if more cob fraction is available, cattle will consume more. There was no treatment effect on final body weight (P = 0.37) or growth rate (P = 0.12) during the Finish phase. Stover NEm and NEg were calculated using Dairy NRC (2001) methods for SPB (1.04 and 0.49 Mcal kg-1) and CST (0.98 and 0.44 Mcal kg-1), respectively. In conclusion, there is evidence that CST and SPB can substitute for WPCS in beef feedlot diets without adverse effects on overall steer performance.

Identification of the Interfacial Surface in Separation of Two-Phase Multicomponent Systems

The area of the contact surface of phases is one of the main hydrodynamic indicators determining the separation and heat and mass transfer equipment calculations. Methods of evaluating this indicator in the separation of multicomponent two-phase systems were considered. It was established that the existing methods for determining the interfacial surface are empirical ones, therefore limited in their applications. Consequently, the use of the corresponding approaches is appropriate for certain technological equipment only. Due to the abovementioned reasons, the universal analytical formula for determining the interfacial surface was developed. The approach is based on both the deterministic and probabilistic mathematical models. The methodology was approved on the example of separation of two-phase systems considering the different fractional distribution of dispersed particles. It was proved that the area of the contact surface with an accuracy to a dimensionless ratio depends on the volume concentration of the dispersed phase and the volume of flow. The separate cases of evaluating the contact area ratio were considered for different laws of the fractional distribution of dispersed particles. As a result, the dependence on the identification of the abovementioned dimensionless ratio was proposed, as well as its limiting values were determined. Finally, a need for the introduction of the correction factor was substantiated and practically proved on the example of mass-transfer equipment.