scholarly journals Tendon collagen synthesis at rest and after exercise in women

2007 ◽  
Vol 102 (2) ◽  
pp. 541-546 ◽  
Author(s):  
Benjamin F. Miller ◽  
Mette Hansen ◽  
Jens L. Olesen ◽  
Peter Schwarz ◽  
John A. Babraj ◽  
...  
Keyword(s):  
Luteal Phase ◽  
Collagen Synthesis ◽  
Synthesis Rate ◽  
Menstrual Phase ◽  
Late Luteal Phase ◽  
Significant Difference ◽  
Healthy Female ◽  
Early Follicular Phase ◽  
Fractional Synthesis ◽  
Tendon Collagen

In general, there is a higher incidence of musculoskeletal injuries during physical activity in women than in men. We hypothesized that in women rates of tendon collagen synthesis would be lower than in men at rest and after exercise, especially in the later luteal phase when estrogen and progesterone concentrations are higher than the early follicular phase. We studied tendon collagen fractional synthesis rate (FSR) in 15 young, healthy female subjects in either the early follicular ( n = 8) or the late luteal phase ( n = 7) 72 h after an acute bout of one-legged exercise (60 min kicking at 67% workload maximum) (72 h) and compared the results with those previously obtained for men. Samples were taken from the patellar tendon in both the exercised and rested legs to determine collagen FSR by the incorporation of [15N]proline into tendon collagen hydroxyproline. There was no effect of menstrual phase on tendon collagen synthesis either at rest or after exercise. However, there was a significant difference between women and men at rest (women = 0.025 ± 0.002%/h, men = 0.045 ± 0.008%/h; P < 0.05) and 72 h after exercise (women = 0.027 ± 0.005%/h; men = 0.058 ± 0.008%/h). Furthermore, rest and 72-h tendon collagen synthesis were not different in women, whereas in men tendon collagen synthesis remained significantly elevated 72 h after exercise. It is concluded that both in the resting state and after exercise, tendon collagen FSR is lower in women than in men, which may contribute to a lower rate of tissue repair after exercise.

scholarly journals A preliminary study exploring the change in ankle joint laxity and general joint laxity during the menstrual cycle in cis women

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Tomomi Yamazaki ◽  
Sae Maruyama ◽  
Yuki Sato ◽  
Yukako Suzuki ◽  
Sohei Shimizu ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Ankle Joint ◽  
Length Change ◽  
Joint Laxity ◽  
Anterior Talofibular Ligament ◽  
Menstrual Phase ◽  
Anterior Drawer ◽  
Linear Distance ◽  
Significant Difference ◽  
Early Follicular Phase

Abstract Background The purpose of the present study was to examine the relationship between ankle joint laxity and general joint laxity (GJL) in relation to the menstrual cycle, which was divided into four phases based on basal body temperature and ovulation, assessed using an ovulation kit. Methods Participants were 14 female college students (21–22 years) with normal menstrual cycles (cis gender). Anterior drawer stress to a magnitude of 120 N was applied for all participants. Anterior talofibular ligament (ATFL) length was measured as the linear distance (mm) between its points of attachment on the lateral malleolus and talus using ultrasonography. Data on ATFL length from each subject were used to calculate each subject’s normalized length change with anterior drawer stress (AD%). The University of Tokyo method was used for evaluation of GJL. AD% and GJL were measured once in each menstrual phase. Results There was no statistically significant difference between AD% in each phase. GJL score was significantly higher in the ovulation and luteal phases compared with the early follicular phase. AD% and GJL showed a positive correlation with each other in the ovulation phase. Conclusions Although it is unclear whether estrogen receptors are present in the ATFL, the present study suggests that women with high GJL scores might be more sensitive to the effects of estrogen, resulting in ATFL length change in the ovulation phase.

scholarly journals Fractional synthesis rates in vivo of skeletal-muscle myosin isoenzymes

1987 ◽  
Vol 245 (1) ◽  
pp. 133-137 ◽  
Author(s):  
P Gregory ◽  
R B Low ◽  
W S Stirewalt
Keyword(s):  
Synthesis Rate ◽  
Single Muscle ◽  
Skeletal Muscle Myosin ◽  
Fast Myosin ◽  
Significant Difference ◽  
Myosin Isoenzymes ◽  
Fractional Synthesis ◽  
Muscle Myosin ◽  
Masseter Muscles

The synthesis rates of different myosin isoenzymes in a single muscle, and of the same isoenzymes in different muscles (soleus, masseter and plantaris), were measured. The rate of total protein synthesis was significantly higher in the soleus [greater than 95% slow myosin (SM)] than in the plantaris [greater than 95% fast myosin (FM)]. Two fast isoenzymes, FM2 and FM3, were synthesized at different rates in the masseter, and SM was synthesized at a faster rate than FM. Intermediate myosin had a synthesis rate similar to that of FM. There was a small but significant difference between the synthesis rates of the SM isoenzymes of the soleus and masseter muscles. FM3 was synthesized faster in the masseter than in the plantaris, whereas FM2 was synthesized faster in the plantaris than in the masseter.

Immunoneutralization and immunocytochemical localization of inhibin α subunit during the mid-luteal phase in the stump-tailed macaque

1992 ◽  
Vol 133 (3) ◽  
pp. 341-NP ◽  
Author(s):  
H. M. Fraser ◽  
K. B. Smith ◽  
S. F. Lunn ◽  
G. M. Cowen ◽  
K. Morris ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Treatment Cycle ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Α Subunit ◽  
Late Luteal Phase ◽  
Early Follicular Phase

ABSTRACT The putative endocrine role of inhibin in the control of FSH secretion during the luteal phase in the primate was investigated by immunoneutralization. Antisera against the 1–23 amino acid sequence of the N-terminus of the human inhibin α subunit were raised in a ewe and three macaques. Antisera (10–20 ml) were administered to macaques on day 8/9 of the luteal phase and serum samples collected during the treatment cycle and post-treatment cycle for determination of FSH, oestradiol and progesterone. In addition, localization of inhibin within the macaque ovary at this stage of the luteal phase was investigated using the ovine antiserum. Intense immunostaining was localized within the granulosa-lutein cells of the corpus luteum with absence of staining in the thecalutein cells or other ovarian compartments. Administration of antisera was without significant effect on serum concentrations of FSH when compared with control animals, either during the first 24 h of detailed observation or for the following 10-day period of the late luteal phase and subsequent early follicular phase. These results provide further evidence that the corpus luteum is the major source of inhibin immunoreactivity during the primate menstrual cycle, but fail to support an endocrine role for inhibin in the suppression of FSH secretion. Journal of Endocrinology (1992) 133, 341–347

scholarly journals GH and IGF1 levels are positively associated with musculotendinous collagen expression: experiments in acromegalic and GH deficiency patients

2010 ◽  
Vol 163 (6) ◽  
pp. 853-862 ◽  
Author(s):  
Simon Doessing ◽  
Lars Holm ◽  
Katja M Heinemeier ◽  
Ulla Feldt-Rasmussen ◽  
Peter Schjerling ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Collagen I ◽  
Synthesis Rate ◽  
Musculoskeletal Tissue ◽  
Collagen Protein ◽  
Fractional Synthesis ◽  
Musculoskeletal Pathology ◽  
Design And Methods ◽  
Tendon Collagen

ObjectiveDisproportionate growth of musculoskeletal tissue is a major cause of morbidity in both acromegalic (ACRO) and GH-deficient (GHD) patients. GH/IGF1 is likely to play an important role in the regulation of tendon and muscle collagen. We hypothesized that the local production of collagen is associated with the level of GH/IGF1.Design and methodsAs primary outcomes, collagen mRNA expression and collagen protein fractional synthesis rate (FSR) were determined locally in skeletal muscle and tendon in nine ACRO and nine GHD patients. Moreover, muscle myofibrillar protein synthesis and tendon collagen morphology were determined.Results and conclusionsMuscle collagen I and III mRNA expression was higher in ACRO patients versus GHD patients (P<0.05), whereas collagen protein FSR did not differ significantly between ACRO and GHD patients in muscle (P=0.21) and tendon (P=0.15).IGF1EaandIGF1EcmRNA expression in muscle was higher in ACRO patients versus GHD patients (P<0.01). MuscleIGF1EamRNA expression correlated positively with collagen I mRNA expression (P<0.01). Tendon collagen fibrillar area tended to be higher in GHD patients relative to ACRO patients (P=0.07). Thus, we observed a higher expression for collagen andIGF1mRNA in local musculotendinous tissue in ACRO patients relative to GHD patients. Moreover, there was a tendency towards a higher collagen protein FSR and a smaller collagen fibril diameter in ACRO patients relative to GHD patients. The results indicate a collagen-stimulating role of local IGF1 in human connective tissue and add to the understanding of musculoskeletal pathology in patients with either high or low GH/IGF1 axis activity.

THE SEQUENCE OF PITUITARY RESPONSES TO SYNTHETIC LUTEINIZING HORMONE RELEASING HORMONE (LH-RH) THROUGHOUT THE NORMAL MENSTRUAL CYCLE

1975 ◽  
Vol 79 (4) ◽  
pp. 625-634 ◽  
Author(s):  
Elwyn M. Grimes ◽  
Irwin E. Thompson ◽  
Melvin L. Taymor
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Follicular Phase ◽  
Lh Surge ◽  
Luteinizing Hormone Releasing Hormone ◽  
Late Luteal Phase ◽  
Normal Menstrual Cycle ◽  
Early Follicular Phase ◽  
Lh Rh ◽  
Late Follicular Phase

ABSTRACT Thirty-one ovulatory women between 20 and 33 years of age were given 150 μg of synthetic LH-RH during different phases of the menstrual cycle. Five patients were studied during the early follicular phase (days 4–7); 10 patients during the late follicular phase (days 9–12); 6 patients during the "LH Surge"; 5 patients during the early luteal phase (days 14–16); 3 patients during mid-luteal phase (days 17–21); and 2 patients during late luteal phase (days 22–27). Oestrogen, progesterone, FSH and LH levels were determined from 30 min prior to LH-RH administration to 90 min thereafter in all cases. LH response to LH-RH increased progressively during the follicular phase. Enhanced pituitary responsiveness to LH-RH occurred at mid-cycle for both LH and FSH and maximum LH responses occurred during the "LH Surge" and early luteal phase. LH responses during the mid and late luteal phases were similar to late follicular phase responses. There were no significant differences between FSH responses during the early follicular, late follicular, mid-luteal and late luteal phases. Maximum pituitary responsiveness appears to occur in a gonadal steroid milieu of high oestrogen levels in association with rising but low progesterone levels. Progesterone or a crucial oestrogen: progesterone ratio may in fact potentiate pituitary release of LH during the early stages of corpus luteum formation. Pituitary responsiveness to LH-RH correlates positively with basal LH and oestrogen levels during the menstrual cycle and with the oestrogen:progesterone ratio during the luteal phase.

scholarly journals Effects of menstrual phase on intake of nicotine, caffeine, and alcohol and nonprescribed drugs in women with late luteal phase dysphoric disorder

1994 ◽  
Vol 6 (2) ◽  
pp. 235-243 ◽  
Author(s):  
Judith L. Marks ◽  
Catherine S. Hair ◽  
Susan C. Klock ◽  
Benson E. Ginsburg ◽  
Cynthia S. Pomerleau
Keyword(s):  
Luteal Phase ◽  
Menstrual Phase ◽  
Late Luteal Phase

Mixed muscle protein synthesis and breakdown after resistance exercise in humans

1997 ◽  
Vol 273 (1) ◽  
pp. E99-E107 ◽  
Author(s):  
S. M. Phillips ◽  
K. D. Tipton ◽  
A. Aarsland ◽  
S. E. Wolf ◽  
R. R. Wolfe
Keyword(s):  
Resistance Exercise ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Exercise Bout ◽  
Synthesis Rate ◽  
Breakdown Rate ◽  
Time Points ◽  
Significant Difference ◽  
Fractional Synthesis ◽  
Exercise In Humans

Mixed muscle protein fractional synthesis rate (FSR) and fractional breakdown rate (FBR) were examined after an isolated bout of either concentric or eccentric resistance exercise. Subjects were eight untrained volunteers (4 males, 4 females). Mixed muscle protein FSR and FBR were determined using primed constant infusions of [2H5]phenylalanine and 15N-phenylalanine, respectively. Subjects were studied in the fasted state on four occasions: at rest and 3, 24, and 48 h after a resistance exercise bout. Exercise was eight sets of eight concentric or eccentric repetitions at 80% of each subject's concentric 1 repetition maximum. There was no significant difference between contraction types for either FSR, FBR, or net balance (FSR minus FBR). Exercise resulted in significant increases above rest in muscle FSR at all times: 3 h = 112%, 24 h = 65%, 48 h = 34% (P < 0.01). Muscle FBR was also increased by exercise at 3 h (31%; P < 0.05) and 24 h (18%; P < 0.05) postexercise but returned to resting levels by 48 h. Muscle net balance was significantly increased after exercise at all time points [(in %/h) rest = -0.0573 +/- 0.003 (SE), 3 h = -0.0298 +/- 0.003, 24 h = -0.0413 +/- 0.004, and 48 h = -0.0440 +/- 0.005], and was significantly different from zero at all time points (P < 0.05). There was also a significant correlation between FSR and FBR (r = 0.88, P < 0.001). We conclude that exercise resulted in an increase in muscle net protein balance that persisted for up to 48 h after the exercise bout and was unrelated to the type of muscle contraction performed.

Endogenous and exogenous female sex hormones and renal electrolyte handling: effects of an acute sodium load on plasma volume at rest

2008 ◽  
Vol 105 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Stacy T. Sims ◽  
Nancy J. Rehrer ◽  
Melanie L. Bell ◽  
James D. Cotter
Keyword(s):  
Plasma Volume ◽  
Oral Contraceptive Pill ◽  
Menstrual Phase ◽  
Plasma Aldosterone Concentration ◽  
Fluid Ingestion ◽  
Female Sex Hormones ◽  
Late Luteal Phase ◽  
Sodium Load ◽  
Early Follicular Phase ◽  
O 10

This study was conducted to investigate effects of an acute sodium load on resting plasma volume (PV) and renal mechanisms across the menstrual cycle of endurance-trained women with natural (NAT) or oral contraceptive pill (OCP) controlled cycles. Twelve women were assigned to one of two groups, according to their usage status: 1) OCP [ n = 6, 29 yr (SD 6), 59.4 kg (SD 3.2)], or 2) NAT [ n = 6, 24 yr (SD 5), 61.3 kg (SD 3.6)]. The sodium load was administered as a concentrated sodium chloride/citrate beverage (164 mmol Na+/l, 253 mosmol/kgH2O, 10 ml/kg body mass) during the last high-hormone week of the OCP cycle (OCPhigh) or late luteal phase of the NAT cycle (NAThigh) and during the low-hormone sugar pill week of OCP (OCPlow) or early follicular phase of the NAT cycle (NATlow). The beverage (∼628 ml) was ingested in seven portions across 60 min. Over the next 4 h, PV expanded more in the low-hormone phase for both groups (time-averaged change): OCPlow 6.1% (SD 1.1) and NATlow 5.4% (SD 1.2) vs. OCPhigh 3.9% (SD 0.9) and NAThigh 3.5% (SD 0.8) ( P = 0.02). The arginine vasopressin increased less in the low-hormone phase [1.63 (SD 0.2) and 1.30 pg/ml (SD 0.2) vs. 1.82 (SD 0.3) and 1.57 pg/ml (SD 0.5), P = 0.0001], as did plasma aldosterone concentration (∼64% lower, P = 0.0001). Thus PV increased more and renal hormone sensitivity was decreased in the low-hormone menstrual phase following sodium/fluid ingestion, irrespective of OCP usage.

scholarly journals Measurement of dermal collagen synthesis rate in vivo in humans

1998 ◽  
Vol 274 (4) ◽  
pp. E586-E591 ◽  
Author(s):  
Wassim A. El-Harake ◽  
Mikhail A. Furman ◽  
Brian Cook ◽  
K. Sreekumaran Nair ◽  
Jayme Kukowski ◽  
...  
Keyword(s):  
Collagen Synthesis ◽  
Current Method ◽  
Synthesis Rate ◽  
Tissue Fluid ◽  
Disease States ◽  
Human Volunteers ◽  
Human Proteins ◽  
Dermal Collagen ◽  
Fractional Synthesis

Accumulation of collagen produces organ dysfunction in many pathological conditions. We measured the fractional synthesis rate (FSR) of dermal collagen in five human volunteers from the increment of [13C]proline in detergent-soluble dermal collagen hydroxylated to hydroxyproline during a continuous infusion ofl-[1-13C]proline. In these and eight other volunteers, we measured [13C]proline enrichment in skin aminoacyl-tRNA, skin tissue fluid amino acid, and plasma. The prolyl-[13C]tRNA enrichment was one-half that in tissue fluid proline and more than threefold less than in plasma. The FSR of dermal collagen was 0.076 ± 0.063%/h (mean ± SD), similar to previously reported rates for skeletal muscle contractile proteins and substantially slower than hepatically derived circulating proteins such as albumin or fibrinogen. We conclude that the FSR of human dermal collagen resembles that of other human proteins considered to display slow turnover. The current method for its measurement may be used to determine the regulation of collagen synthesis in other organs and disease states.

Local administration of growth hormone stimulates tendon collagen synthesis in elderly men

2012 ◽  
Vol 113 (9) ◽  
pp. 1432-1438 ◽  
Author(s):  
Poul Vestergaard ◽  
Jens Otto Lunde Jørgensen ◽  
Jens L. Olesen ◽  
Ermina Bosnjak ◽  
Lars Holm ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Collagen Synthesis ◽  
Synthesis Rate ◽  
Saline Control ◽  
Type I ◽  
Elderly Men ◽  
Healthy Elderly ◽  
Igf I ◽  
Local Injections ◽  
Tendon Collagen

Tendon collagen content and circulating growth hormone (GH) are reduced in elderly. In a placebo-controlled, double-blinded study, we examined if local injections of rhGH enhance collagen synthesis in healthy elderly men (61 ± 1 yr). Two injections of rhGH or saline (control) were injected into each of the patient's patellar tendons, respectively. Subsequently, tendon collagen fractional synthesis rate (FSR) and an indirect marker of type I collagen synthesis (PINP) were measured. Within the first 6 h after the last injections, a tendency towards a higher tendon collagen FSR was observed in 10 out of 12 subjects ( P = 0.08). Similarly, PINP was higher 3–4 h after the last GH injection ( P = 0.05). Serum IGF-I did not change from baseline, whereas peritendinous bioactive IGF-I was higher in the GH leg vs. control ( P = 0.05). In conclusion, local injections of rhGH increase tendon collagen synthesis in humans, either directly or indirectly by increasing local bioactive IGF-I.

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