scholarly journals Biological Characterisation of Superficial Bladder Cancer by Bivariate Cytokeratin 7/DNA Analysis, Flow Cytometric Assessment of MIB-1, and an Immunohistochemical Study

2000 ◽  
Vol 21 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Elena Leonardi ◽  
Paolo Dalla Palma ◽  
Alessandro Reich ◽  
Orazio Caffo ◽  
Lucio Luciani
Keyword(s):  
Bladder Cancer ◽  
Dna Content ◽  
Dna Analysis ◽  
Immunohistochemical Study ◽  
Statistical Significance ◽  
Multiparameter Flow Cytometry ◽  
Cytokeratin 7 ◽  
Biological Behaviour ◽  
Flow Cytometric ◽  
Mib 1

A total of 238 cases of bladder carcinoma stages Ta, Tis, T1 were submitted prospectively to multiparameter flow cytometry and immunohistochemical study in order to determine the biological aggressiveness of the tumour. DNA index (DI), S-phase fraction (SPF) obtained by bivariate cytokeratin 7=DNA analyses, and the immunohistochemical evaluation of p53 and MIB-1 were studied in relation to the traditional prognostic factors in bladder cancer (stage and grade). The variance analysis results showed that DNA aneuploidy was significantly associated with high stage (p= 0:0001), high grade (p= 0:0001), high SPF value ≥5.5% (p= 0:0001), MIB-1 positivity ≥31% (p= 0:0001) and high expression of p53 (staining involving >50% of cells,p= 0:0001). Even if there was no statistical significance the hypotetraploid class (1.70<DI<1.89) showed poor prognostic biomarkers more frequently than the other aneuploid classes. Out of 238 cases, 101 were also submitted to flow cytometric measurement ofMIB-1 (fMIB-1) to study the correlation between cell proliferation and DNA content. Data obtained from fresh, 3 : 1 methanol=acetone fixed samples were compared with values obtained from both cell cycle analysis methods and routine application of the MIB-1 immunostaining in histological sections. fMIB-1 values were positively correlated with SPF values (r= 0:801,p< 0:01) and S+G2M fraction (percentage of cells in S and in G2M phases) (r= 0:763,p< 0:01) but no correlation with paraffin sections was found. A fMIB-1 value >7% was strongly associated with aneuploidy (p= 0:0001). The determination of DNA content coupled with the study of the epithelial (cytokeratin 7) and proliferative (MIB-1) markers could be useful in providing important information on the biological behaviour of superficial bladder tumours.

Limitations of flow cytometric DNA analysis for the diagnosis of bladder cancer

1989 ◽  
Vol 40 (3) ◽  
pp. 194-200 ◽  
Author(s):  
Michel A. Boileau ◽  
Douglas Swartz ◽  
Karmen L. Schmidt ◽  
Waldemar A. Schmidt
Keyword(s):  
Bladder Cancer ◽  
Dna Analysis ◽  
Flow Cytometric

FLOW CYTOMETRIC DNA ANALYSIS IN PREDICTION OF INTRAVESICAL RECURRENCE FROM HUMAN BLADDER-CANCER

1994 ◽  
Author(s):  
A RUSSO ◽  
V BAZAN ◽  
R CAMPANELLA ◽  
G DARDANONI ◽  
I CARRECA ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Dna Analysis ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Flow Cytometric

scholarly journals Electrofusion of protoplasts of anther-derived dihaploid lines of commercial potato cultivars

1996 ◽  
Vol 5 (4) ◽  
pp. 449-460 ◽  
Author(s):  
Veli-Matti Rokka ◽  
Yong-Sheng Xu ◽  
Pirjo Tanhuanpää ◽  
Leena Pietilä ◽  
Eija Pehu
Keyword(s):  
Dna Content ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Somatic Hybrids ◽  
Solanum Tuberosum L ◽  
Rapd Marker ◽  
Nuclear Dna Content ◽  
Parental Line ◽  
Flow Cytometric ◽  
Commercial Potato

Somatic hybrids of anther-derived dihaploid (2x) potato (Solanum tuberosum L.) lines were produced by electrofusion of protoplasts. Using RAPD (randomly amplified polymorphic DNA) markers, six new combinations of dihaploid parental lines from cultivars Matilda, Nicola, Pito, Stina, Van Gogh and White Lady were identified. RAPD marker identification of the putative hybrids was mostly done using two distinct parental line specific primers. 43% of the 76 regenerated calli from the six combinations produced hybrid shoots. Most of the somatic hybrids were tetraploid (4x), but in four fusion combinations plants of hexaploid (6x), octoploid (8x) or mixoploid level were also identified by chromosome counts or flow cytometric nuclear DNA analysis. The mean nuclear DNA content (2C value) of the tetraploid and hexaploid somatic hybrids was lower than the expected DNA content (i.e. the 2C values of the original tetraploid cultivars or the sum of the 2C values of the dihaploid fusion parents). Some somatic hybrids having the expected nuclear DNA content were also found.

Sinonasal Hemangiopericytoma: DNA Content and Pathological Features

1996 ◽  
Vol 10 (1) ◽  
pp. 39-44
Author(s):  
Charles J. Wilson ◽  
Fernando U. Garcia ◽  
Larry A. Hoover
Keyword(s):  
Metastatic Disease ◽  
Dna Content ◽  
Dna Analysis ◽  
Smooth Muscle Actin ◽  
Prognostic Significance ◽  
Initial Therapy ◽  
Low Grade ◽  
Pathological Features ◽  
Local Recurrences ◽  
Mib 1

Hemangiopericytomas are rare vasoformative tumors representing varied clinical courses and histology. Sinonasal hemangiopericytomas (SNHP) are thought to be low grade malignancies with possible late local recurrences and rare metastases even after therapy. We present four cases of SNHP and evaluate the pathological features of diagnostic and prognostic significance. Our cases are compared with all previously reported SNHP series. Cases were retrieved from medical records from our teaching institutions for the last 20 years. Clinical presentation, course, and pathology were reviewed in all cases. DNA content by image analysis, proliferation antigen (MIB-1), estrogen and progesterone receptor status, and other immunohistochemical markers were performed. All patients were females with mean age at presentation of 48.5 (18–77) years. Initial therapy was surgery alone or in combination with radiation therapy. Three patients had local recurrences at 5, 9, and 10 years, one of whom died of metastatic disease 11 years after diagnosis. DNA analysis revealed that three patients had diploid tumors. The patient who died of metastatic disease had a large aneuploid population. The proliferative fraction of the tumors studied with MIB-1 was less than 5% in all tumors. Estrogen receptors were negative in all tumors, and two tumors showed 10% positivity for progesterone receptors. All tumors stained positively with antibodies to vimentin and variably positive with smooth muscle actin. SNHP patients require life-long follow-up because of possible late recurrences and the potential for metastasis. DNA content may be useful for identifying malignant potential.

DNA content and proliferative activity of myoepitheliomas

1989 ◽  
Vol 103 (12) ◽  
pp. 1192-1197 ◽  
Author(s):  
A. El-Naggar ◽  
J. G. Batsakis ◽  
M. A. Luna ◽  
H. Goepfert ◽  
M. E. Tortoledo
Keyword(s):  
Dna Content ◽  
Proliferative Activity ◽  
Clinical Evidence ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Proliferative Capacity ◽  
Biological Behaviour ◽  
Flow Cytometric ◽  
First Time

AbstractThis report adds 16 myoepitheliomas of salivary glands to the 47 already recorded in the literature. It includes, for the first time, a flow cytometric analysis of their ploidy (DNA content) and proliferative capacity (S-phase fraction). Thirteen myoepitheliomas were diploid; three were aneuploid in their DNA content. A high proliferative capacity was always associated with an abnormal DNA content. Only one diploid myoepithelioma had a high S-phase fraction. Both flow-cytometric parameters are good predictors of an aggressive biological behaviour. Recurrences, however, were all the outcome of incomplete primary removal of the myoepitheliomas. Four of the twelve (33 per cent) diploid myoepitheliomas recurred and one, with high S-phase fraction, led to the death of the patient. Two of the three (67 per cent) aneuploid myoepitheliomas recurred. Extensive loco-regional invasion by one killed the patient. The other has clinical evidence of distant metastasis.

scholarly journals Prognostic significance of dna content in bladder cancer based on flow cytometric analysis of 249 transitional cell carcinomas

Cytometry ◽  
1995 ◽  
Vol 22 (2) ◽  
pp. 93-102 ◽  
Author(s):  
Lars L. Vindeløv ◽  
I. J. Christensen ◽  
Svend A. Engelholm ◽  
Birgitte H. Guldhammer ◽  
Knud Højgaard ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Dna Content ◽  
Transitional Cell ◽  
Prognostic Significance ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric
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