scholarly journals The Relationship between Syntactic Structure Analysis Features, Histological Grade and High-Risk HPV DNA in Cervical Intraepithelial Neoplasia

2004 ◽  
Vol 26 (3) ◽  
pp. 135-141
Author(s):  
Arnold‐Jan Kruse ◽  
Susanne Buhr‐Wildhagen ◽  
Emiel A. Janssen ◽  
Jan P.A. Baak
Keyword(s):  
High Risk ◽  
Cervical Intraepithelial Neoplasia ◽  
Structure Analysis ◽  
Syntactic Structure ◽  
Surrogate Marker ◽  
Intraepithelial Neoplasia ◽  
Stepwise Discriminant Analysis ◽  
Hpv Dna ◽  
High Risk Hpv ◽  
Syntactic Structure Analysis

Aim: To assess the correlation between syntactic structure analysis (SSA) features, revised dysplasia grade and the presence of high‐risk human papillomavirus DNA in cervical intraepithelial neoplasia (CIN). Materials and methods: HPV polymerase chain reaction (PCR) was assessed in 101 consecutive biopsies and consensus in CIN grade between the experts occurred in 88 cases (CIN1=16, CIN2=27, CIN3=45). SSA was performed in the diagnostic histological section of the CIN lesions in these patients and SSA features were compared with the blind review CIN grade, and presence/absence of high‐risk HPV DNA. Results: One of the SSA features (points from which the surrounding surfaces has 4 edges, PECO‐4) was significantly different between all three consensus CIN grades. Many more features revealed significant differences between CIN1 and CIN2 or between CIN2 and CIN3 cases. With stepwise discriminant analysis, the best multivariate combination of features to distinguish the different CIN grades were the Maximum MST Line Length (MML) and the Area Disorder. Crude overall classification of the consensus grades with these features was 69%. The MML and the Area Disorder is also the best combination to distinguish cases with and without high‐risk HPV DNA (77% correct classifications). Conclusions: SSA features are correlated with both CIN grade and presence of high‐risk HPV DNA, but the discrimination power is not good enough to be used as a routine method for quality control of subjective grade or as a surrogate marker for high‐risk HPV DNA presence.

Cigarette smoking and high-risk HPV DNA as predisposing factors for high-grade cervical intraepithelial neoplasia (CIN) in young Brazilian women

1998 ◽  
Vol 77 (6) ◽  
pp. 678-682 ◽  
Author(s):  
Cecilia Maria Roteli-Martins ◽  
Kazue Panetta ◽  
Venancio Avancini Ferreira ALVES ◽  
Sheila Aparecida Coelho Siqueira ◽  
Kari Juhani Syrjänen ◽  
...  
Keyword(s):  
High Risk ◽  
Cigarette Smoking ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Predisposing Factors ◽  
High Grade ◽  
Hpv Dna ◽  
High Risk Hpv

Comparison of Human Papillomavirus Detection and Typing by Hybrid Capture 2, Linear Array, DNA Chip, and Cycle Sequencing in Cervical Swab Samples

2009 ◽  
Vol 19 (2) ◽  
pp. 266-272 ◽  
Author(s):  
Jae Kwan Lee ◽  
Mi Kyung Kim ◽  
Seung Hun Song ◽  
Jin Hwa Hong ◽  
Kyung Jin Min ◽  
...  
Keyword(s):  
High Risk ◽  
Linear Array ◽  
Intraepithelial Neoplasia ◽  
Dna Chip ◽  
Substantial Agreement ◽  
Cervical Lesions ◽  
Cycle Sequencing ◽  
Hpv Dna ◽  
Hpv Dna Tests ◽  
High Risk Hpv

Although the Hybrid Capture II (HC II) assay can detect 13 high-risk human papillomavirus (HPVs), it does not yield any genotype-specific information. We evaluated the performance of 4 HPV DNA tests, namely, HC II, Linear Array (LA), DNA chip, and cycle sequencing for their capacity to detect the presence of high-risk HPV DNA and HPV-associated cervical lesions. Seventy-six women who were referred to the colposcopy clinic for abnormal cytology were enrolled. The women were examined using liquid-based cytology, colposcopy-directed biopsy, and HPV DNA tests. After DNA extraction from a single sample, HPV DNA tests were performed by all 4 methods on the same specimen. The LA test has higher HPV-positive rates than HC II for cervical intraepithelial neoplasia I (83.3% vs 61.1%;P< 0.01) and for cervical intraepithelial neoplasia II and more severe lesions (100.0% vs 80.0%;P< 0.01). The concordance between the DNA chip and LA tests was 89.5%, confirming substantial agreement (κcoefficient = 0.73), and the concordance between HC II and the DNA chip was 80.3%, also showing substantial agreement (κcoefficient = 0.738). The concordance for 15 high-risk HPV genotypes between LA and sequencing was 82.5% with aκvalue of 0.536. Furthermore, the LA test was more sensitive in the detection of high-grade cervical lesions than HC II (100% vs 92.3%,P< 0.01). The LA test showed superior sensitivity in the detection of clinically relevant HPV infections and has proven to be an accurate tool for identifying individual HPV types, especially in cases of multiple HPV infections.

High risk HPV and p53 protein expression in cervical intraepithelial neoplasia

2000 ◽  
Vol 71 (1) ◽  
pp. 45-48 ◽  
Author(s):  
J. Vassallo ◽  
S.F.M. Derchain ◽  
G.A. Pinto ◽  
E.Z. Martinez ◽  
K.J. Syrjänen ◽  
...  
Keyword(s):  
High Risk ◽  
Protein Expression ◽  
Cervical Intraepithelial Neoplasia ◽  
P53 Protein ◽  
Intraepithelial Neoplasia ◽  
P53 Protein Expression ◽  
High Risk Hpv

scholarly journals Addition of high-risk HPV testing improves the current guidelines on follow-up after treatment for cervical intraepithelial neoplasia

2001 ◽  
Vol 84 (6) ◽  
pp. 796-801 ◽  
Author(s):  
M A E Nobbenhuis ◽  
C J L M Meijer ◽  
A J C van den Brule ◽  
L Rozendaal ◽  
F J Voorhorst ◽  
...  
Keyword(s):  
High Risk ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Hpv Testing ◽  
High Risk Hpv ◽  
Current Guidelines

scholarly journals Correlation between Human Papillomavirus Codetection Profiles and Cervical Intraepithelial Neoplasia in Japanese Women

2020 ◽  
Vol 8 (12) ◽  
pp. 1863
Author(s):  
Kaori Okayama ◽  
Hirokazu Kimura ◽  
Koji Teruya ◽  
Yasuyoshi Ishii ◽  
Kiyotaka Fujita ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Hpv Genotypes ◽  
High Risk Type ◽  
Pcr Method ◽  
Polymerase Chain ◽  
High Risk Hpv

Human papillomavirus (HPV) infection is thought to be strongly associated with the precarcinomatous state cervical intraepithelial neoplasia (CIN) and cervical carcinoma. To accurately assess the correlation between HPV detection profiles and CIN, the uniplex E6/E7 polymerase chain reaction (PCR) method was used. We detected HPV (37 genotypes) in 267 CIN cases. The detection of a single high-risk HPV genotype occurred in 69.7% of CIN1 and worse than CIN1 (CIN1+) cases whereas other types were detected in 11.6% of cases. Codetection of high-risk HPV genotypes occurred in 4.9% of CIN1+ cases. The high-risk genotype HPV16 was the most frequently detected genotype in CIN1+ lesions; the genotype HPV34 (not a high-risk type) was detected in some CIN3 cases. Furthermore, HPV codetection may not be associated with CIN grades. These results suggest that various HPV genotypes are associated with CIN across all analyzed cases.

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