Production and Preliminary Characterization of Alkaline Protease fromAspergillus flavusandAspergillus terreus

Proteases are being an industrial candidate, which are widely used in food, bakery, and beverage and detergent industry. In leather industry, alkaline proteases are exhibiting a prominent role in unhairing and bating processes. An extensive use of filamentous fungi, especiallyAspergillus specieshas been studied elaborately. Although, the significant application of alkaline protease produced from these strains in leather industry is being limited.Aspergillus flavusandAspergillus terreusfound as the potential strains for production of tannery protease in submerged fermentation. To improve the productivity of this enzyme in liquid broth, various media ingredients have been optimized. The crude and partially purified proteases preliminarily characterized and used for unhairing processes at lab scale in tannery. The protease obtained from these strains showed the good activity in wide alkaline condition at 50 °C suggesting the possibility of using in leather and detergent industry.

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Biochemical characterization of a partially purified protease from Aspergillus terreus 7461 and its application as an environmentally friendly dehairing agent for leather industry

Preparative Biochemistry & Biotechnology ◽

10.1080/10826068.2020.1815058 ◽

2020 ◽

pp. 1-11

Author(s):

Emmly Ernesto de Lima ◽

Daniel Guerra Franco ◽

Rodrigo Mattos Silva Galeano ◽

Nelciele Cavalieri de Alencar Guimarães ◽

Douglas Chodi Masui ◽

...

Keyword(s):

Aspergillus Terreus ◽

Biochemical Characterization ◽

Environmentally Friendly ◽

Leather Industry

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In silico analysis to elect superior bacterial alkaline protease for detergent and leather industries

JOURNAL OF ADVANCES IN BIOTECHNOLOGY ◽

10.24297/jbt.v5i3.1482 ◽

2015 ◽

Vol 5 (3) ◽

pp. 685-698 ◽

Cited By ~ 3

Author(s):

Kamonashis Das ◽

Sourav Chakraborty ◽

Mahmudul Hasan ◽

Abdullah Maruf Rahman Shovo

Keyword(s):

High Temperature ◽

Bacillus Megaterium ◽

In Silico ◽

Alkaline Protease ◽

Protein Sequences ◽

In Silico Analysis ◽

Alkaline Proteases ◽

Leather Industry ◽

Catalytic Function ◽

Common Motif

Alkaline protease contributes 40% of the total worldwide enzyme sales. Alkaline protease that is stable at very high temperature and pH is massively desirable for detergent industry and leather industry specially in tanning process. So the present study aims to elect superior bacterial alkaline protease (high temperature and pH stable) as compared to the alkaline proteases of currently industrially used bacteria (Bacillus subtilis and Bacillus cereus). A total of 50 protein sequences of alkaline proteases of different bacteria were analyzed through in silico characterization. ProtParam result revealed that isoelectric point and aliphatic index of alkaline protease of Bacillus megaterium were 8.83 and 93.35 respectively. In case of alkaline protease of B. megaterium, these two properties were significant in comparison to alkaline proteases of industrially used bacteria and other considered bacteria. A common motif of 28 amino acid residues i.e., IQSTYPGEDYEYMSGTSMATPHVAGVAA was found using MEME software in 46 protein sequences. It can be concluded that alkaline protease of Bacillus megaterium may be superior to alkaline proteases of industrially used bacteria and other considered bacteria. In addition, obtained common motif indicates its probable role in catalytic function and structure of alkaline proteases.Â

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Isolation and Characterization of Alkaline Protease Producing Streptomyces tendae SO-13 from Rhizosphere Soils of Western Ghats

Bioscience Biotechnology Research Communications ◽

10.21786/bbrc/13.4/93 ◽

2020 ◽

Vol 13 (4) ◽

pp. 2249-2254

Author(s):

Subbaraju A

Keyword(s):

Alkaline Protease ◽

Western Ghats ◽

Rhizosphere Soils ◽

Isolation And Characterization ◽

Streptomyces Tendae

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Purification and characterization of organic solvent stable serine alkaline protease from newly isolatedBacillus circulansM34

Biomedical Chromatography ◽

10.1002/bmc.3431 ◽

2015 ◽

Vol 29 (9) ◽

pp. 1356-1363 ◽

Cited By ~ 10

Author(s):

Esma Sari ◽

Elif Loğoğlu ◽

Atilla Öktemer

Keyword(s):

Organic Solvent ◽

Alkaline Protease ◽

Purification And Characterization ◽

Serine Alkaline Protease ◽

Solvent Stable

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Characterization of the Maize Chitinase Genes and Their Effect on Aspergillus flavus and Aflatoxin Accumulation Resistance

PLoS ONE ◽

10.1371/journal.pone.0126185 ◽

2015 ◽

Vol 10 (6) ◽

pp. e0126185 ◽

Cited By ~ 33

Author(s):

Leigh K. Hawkins ◽

J. Erik Mylroie ◽

Dafne A. Oliveira ◽

J. Spencer Smith ◽

Seval Ozkan ◽

...

Keyword(s):

Aspergillus Flavus ◽

Aflatoxin Accumulation ◽

Chitinase Genes

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Extraction, partial purification and characterization of alkaline protease from rainbow trout (Oncorhynchus Mykiss) viscera

Aquaculture ◽

10.1016/j.aquaculture.2018.10.052 ◽

2019 ◽

Vol 500 ◽

pp. 458-463 ◽

Cited By ~ 2

Author(s):

Ghasem Taghizadeh Andevari ◽

Masoud Rezaei ◽

Mehdi Tabarsa ◽

Turid Rustad

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Alkaline Protease ◽

Partial Purification ◽

Purification And Characterization ◽

Rainbow Trout Oncorhynchus Mykiss

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Mycoflora in Commercial Pet Foods

Journal of Food Protection ◽

10.4315/0362-028x-64.5.741 ◽

2001 ◽

Vol 64 (5) ◽

pp. 741-743 ◽

Cited By ~ 13

Author(s):

DANTE J. BUENO ◽

JULIO O. SILVA ◽

GUILLERMO OLIVER

Keyword(s):

Aspergillus Niger ◽

Moisture Content ◽

Aspergillus Flavus ◽

Aspergillus Terreus ◽

Animal Health ◽

Mold Growth ◽

Mucor Racemosus ◽

Isolation Frequency ◽

Mycelia Sterilia ◽

Made In

This article reports on the identification of mycoflora of 21 dry pet foods (12 belonging to dogs and 9 to cats) that corresponded to 8 commercial brands made in Argentina and imported. The isolation frequency and relative density of the prevalent fungal genera are compared too. Ten genera and fungi classified as Mycelia sterilia were identified. The predominant genera were Aspergillus (62%), Rhizopus (48%), and Mucor (38%). The most prevalent among Aspergillus was Aspergillus flavus followed by Aspergillus niger and Aspergillus terreus. The predominant Mucor was Mucor racemosus followed by Mucor plumbeus and Mucor globosus. The moisture content of these foods ranged from 5.6 to 10.0% and from 7.2 to 9.9% for dog and cat foods, respectively. A greater moisture content in food for the senior category (9.5 ± 0.2) was observed only in comparison to adult and kitten/puppy. If the moisture content can be maintained at these levels, mold growth would be prevented or at least it would remain at an insignificant level. Some genera and species isolated and identified from the foods analyzed are potentially producing toxins, which are known as mycotoxins. This involves a risk for animal health.

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