PLA Biocomposites: Evaluation of Resistance to Mold

Due to the content of lignocellulosic particles, wood plastic composites (WPC) composites can be attacked by both domestic and mold fungi. Household fungi reduce the mechanical properties of composites, while mold fungi reduce the aesthetics of products by changing their color and surface decomposition of the wood substance. As part of this study, the impact of lignocellulosic fillers in the form of sawdust and bark in poly (lactic acid) (PLA)-based biocomposites on their susceptibility to mold growth was determined. The evaluation of the samples fouled with mold fungi was performed by computer analysis of the image. For comparison, tests were carried out on analogous high-density polyethylene (HDPE) composites. Three levels of composites’ filling were used with two degrees of comminution of lignocellulosic fillers and the addition of bonding aids to selected variants. The composites were produced in two stages employing extrusion and flat pressing. The research revealed that PLA composites were characterized by a higher fouling rate by Aspergillus niger Tiegh fungi compared to HDPE composites. In the case of HDPE composites. The type of filler (bark, sawdust) affected this process much more in the case of HDPE composites than for PLA composites. In addition, the use of filler with smaller particles enhanced the fouling process.

Quality Parameters of Wheat Bread with the Addition of Untreated Cheese Whey

Τhe present study was carried out to evaluate wheat bread of three different flour compositions prepared by replacing water with untreated cheese whey (WCB). Bread prepared with water was taken as the control (CB). All breads were stored at 24 ± 1 °C for up to 6 days. Microbiological, physicochemical, and sensory analyses were determined as a function of storage time. WCB had lower total viable counts (TVC) (3.81 log cfu/g for CB and 2.78 log cfu/g for WCB on day 2 of storage) and showed delayed mold growth by 1 day (day 4 for CB and day 5 for WCB). WCB also had lower pH (5.91 for CB and 5.71 for WCB on day 0), higher titratable acidity values (TTA) (2.5–5.2 mL NaOH/10 g for CB and 4.5–6.8 mL NaOH/ 10 g for WCB), and higher protein content (PC) (PC 7.68% for CB and 8.88% for WCB). WCB was characterized by a more intense flavor, reduced hardness but similar cohesiveness, springiness, and adhesiveness compared to CB. Based primarily on sensory (appearance/mold formation) data, the shelf life of WCB was 4–5 days compared to 3–4 days for CB stored at 24 ± 1 °C. The proposed use of whey in bread preparation contributes decisively to the environmentally friendly management of whey.

Influence of the mold growth on the crystallographic composition of hemp mortar

The use of hemp mortar as a bio-based insulation composite is widely promoted in the construction sector in France due to its environmental and hygrothermal advantages and the availability and low price of hemp fibers. Nevertheless, the use of such materials claims the consideration of the microbiological contamination that could lead to its degradation. Molds are known for their ability to modify locally the composition of hemp mortar by decreasing the pH level. That’s why the main objectives of the present work are, first, to expose the hemp mortar favorable conditions for mold growth, secondly, to investigate the proliferation of the mold filaments inside the hemp mortar sample and, then, to analyze the crystallographic composition. Experimentally, hemp mortar samples were exposed to high level of relative humidity during one year until the mold growth. The SEM observation allowed to follow the internal growth and identify the depth of the mold growth. Finally, the composition of the contaminated hemp mortar was studied by X-ray diffraction. The obtained results reveal that molds growth occurs not only on the surface but also in the depth. Nevertheless, as the mold growth started only after one year of high humidity exposure, a good resistance of studied hemp mortar towards molds was noted. Furthermore, the mineralogical composition analysis of the contaminated samples shows that the hydrates responsible for durability remained. These results provide data to better predict the durability of hemp mortars.

The use of an internal airtight membrane in CLT external wall in terms of hygrothermal performance

This study focused on the dry-out capacity of the vapor-permeable CLT (cross-laminated timber) external wall and the impact of using an internal airtight membrane. The results of the work were obtained first from the field measurements, after which the simulation model was created and validated, and the hygrothermal performance of the wall was analyzed by a stochastic approach. The results of this showed that the CLT dries out quickly and safely in a wall assembly with a high water vapor permeability, even with the large range of initial CLT MC (13-25%). When an additional airtight layer with high vapor diffusion resistance (Sd of 244 m) is added between the insulation and the CLT, the dry-out capacity of the CLT decreases significantly and there is a high probability of mold growth on the CLT surface. The risk of mold growth can be prevented when the vapor resistance (Sd) of the airtight layer is reduced to 1.5 m in a case where initial CLT MC is up to 25% and in a case where initial MC is up to 20%, the vapor resistance of an airtight layer must be reduced to 3 m.

Effects of climate change on the moisture performance and durability of brick veneer walls of wood frame construction in Canada

The objective of this study was to assess the potential effects of climate change on the moisture performance and durability of red matt clay brick veneer walls of wood frame construction on the basis of results derived from hygrothermal simulations. One-dimensional simulations were run using DELPHIN 5.9 for selected moisture reference years of the 15 realizations of modelled historical (H: 1986-2016) and future (F: 2062-2092) climate data of 12 Canadian cities. The mold growth index at the outer layer of the OSB sheathing panel was used to compare the moisture performance under H and F periods. Results for the base design that meet the minimum requirements of the National Building Code of Canada showed that cities within the interior of the country, characterized by a low annual rainfall, are less likely to develop significant mold growth under H and F periods, whereas cities in coastal areas, characterized by high annual rainfall, present a heightened risk to mold growth under both H and F periods. For cities located on the west coast, a possible solution could be to use a 38-mm ventilated drainage cavity as this measure would help dissipate moisture from within the cavity. On the east coast, apart from using a 38-mm ventilated drainage cavity, other measures aiming at reducing the wind-driven rain deposition (i.e., increasing overhang ratio or the height of the roof) could be introduced. However, the feasibility of such measures needs to be considered in respect to whether these are to be implemented as part of a new building or retrofit of an existing one.

Effects of different light wavelengths on mold growth in tomb

The Second Mausoleum of the Southern Tang Dynasty have a history of more than 1,000 years in China. Since its opening in 1984, the building materials and murals inside the tomb have been severely damaged by mold. Field investigation found that the mold growth on the wall illuminated by the light in the tomb was more flourishing than that in the area without the light. Lighting in the tomb is inevitable for the exhibition. Therefore, the purpose of this study is to clarify the effects of different light wavelengths on the growth of mold in the tomb, and provide the theoretical basis for the lighting design in the tomb chamber. This study is divided into two parts, including in-situ experiment and laboratory experiment. In the in-situ experiment, four kinds of light wavelengths (white, red, blue, and green) were set in the tomb chamber to observe the difference of mold growth on the mural wall. The concentration of phototrophs propagules on different auricular walls was estimated in the laboratory. The results showed that different light sources had different effects on microbial community composition in the tomb site and the red light had better inhibition effect on fungi and actinobacteria.

Nutritional and Fungal Load Dynamics of Fresh Brewers’ Grain Stored Under Aerobic Conditions

Brewers’ spent grain (BSG) is the amplest by-product of the brewing process. Fresh BSG is currently used as low-cost cattle feed due to its microbiological instability and high perishability. While recent research has looked the effects of storage time and temperature on the characteristics of wet brewers grains (WBG) as ruminant feeds. Three storage temperatures (15°C, 20°C, and 25°C) and periods (2, 4 and 6 days) were arranged in a 3×3 factorial design. Surface spoilage was not apparent at 15 °C throughout the storage periods. Deterioration was not also observed at 20 °C until the fourth day of storage where slight mold growth was apparent. Extensive mold growth was detected late in the sixth day at 20° C and continued manifestations up until the last day of storage at 25°C. Changes in major nutrients, DM losses, and yeast and mold colony count were significantly affected by the interaction of storage temperatures and durations (P<0.05). Except for samples stored at 15° C, nutrients contents decreased concomitantly (exceptions are ADF, lignin, and loss in DM) with prolonged storage times (p<0.05) and increasing temperatures (p<0.05). Contrast analysis indicated that it would be safe to store under aerobic storage conditions and feed the WBG for dairy cattle.

Toward an Understanding of Physical and Biological Properties of Corn-Based Whole Stillage, Thin Stillage, and Condensed Distillers Solubles and Changes Thereof During Storage

The production of bio-based ethanol has been one of the fastest growing industries in the United States during the past decade. Thus, wider exploration of ethanol coproduct uses is necessary in the ethanol plant. Currently, process steams such as whole stillage, thin stillage, and syrup are processed into distiller dried grains with solubles and fed to livestock. The storability of whole stillage, thin stillage, and syrup influences the economic and energetic balances of fuel ethanol production. However, there are few investigations of the shelf life for these products or how to measure these quantities. The objectives of this research were to test physical and biological properties of whole stillage, thin stillage, and syrup and determine storability and allowable shelf life for these materials as influenced by storage temperature levels. Using standard laboratory methods, several properties were determined, including moisture content, water activity, thermal properties (conductivity, resistivity, volumetric heat capacity, and diffusivity), color, mold development, and CO2 production. Also, the separation processes due to settling were observed over 72 h. The thin stillage and whole stillage had relative high average moisture contents of 92% (w.b.) and 87% (w.b.), respectively, and a mean water activity of 0.99; the high water content marked samples easily susceptible to rapid spoilage. Time had a significant effect (p &lt; 0.05) on properties of coproducts. Both thin stillage and whole stillage samples got mold growth after 5 days incubation at 32°C. Thin stillage had the greatest separation rate in the settling experiment. However, syrup had a relative low average moisture content of 62% and an average water activity of 0.92. No mold growth and settling separation happened in syrup samples. There was no evidence showing that a linear relationship exists between Hunter L*, a* and b*, and mold growth. The Solvita® test showed that high-temperature treatment caused high CO2 production in all samples. The exponential models described the relationship between storage time (from 0 to 5 days at 25 and 35°C) and CO2 concentration for the three coproducts. This study is a first step to explore opportunities for utilizing valuable components from these coproducts. Follow-up study should work on separation processes to concentrate the valuable components of these coproducts. Exploring the potential value of ethanol coproducts could maintain and improve the profitability of the ethanol industry.