Metabolomic Characterization of Ovarian Epithelial Carcinomas by HRMAS-NMR Spectroscopy

Objectives. The objectives of the present study are to determine if a metabolomic study by HRMAS-NMR can (i) discriminate between different histological types of epithelial ovarian carcinomas and healthy ovarian tissue, (ii) generate statistical models capable of classifying borderline tumors and (iii) establish a potential relationship with patient's survival or response to chemotherapy.Methods. 36 human epithelial ovarian tumor biopsies and 3 healthy ovarian tissues were studied using1H HRMAS NMR spectroscopy and multivariate statistical analysis.Results. The results presented in this study demonstrate that the three histological types of epithelial ovarian carcinomas present an effective metabolic pattern difference. Furthermore, a metabolic signature specific of serous (N-acetyl-aspartate) and mucinous (N-acetyl-lysine) carcinomas was found. The statistical models generated in this study are able to predict borderline tumors characterized by an intermediate metabolic pattern similar to the normal ovarian tissue. Finally and importantly, the statistical model of serous carcinomas provided good predictions of both patient's survival rates and the patient's response to chemotherapy.Conclusions. Despite the small number of samples used in this study, the results indicate that metabolomic analysis of intact tissues by HRMAS-NMR is a promising technique which might be applicable to the therapeutic management of patients.

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Correlation of CD44v6 and type IV collagen immunohistochemical expression in borderline and malignant surface epithelial tumors

AL-Kindy College Medical Journal ◽

10.47723/kcmj.v12i2.299 ◽

2016 ◽

Vol 12 (2) ◽

pp. 34-42

Author(s):

Mohanad M. Abdul Ghany ◽

Mohammed A. AlKurtas ◽

Israa T. Hassan

Keyword(s):

Type Iv Collagen ◽

Invasive Carcinoma ◽

Histological Grade ◽

Ovarian Tissue ◽

Inverse Correlation ◽

Ovarian Carcinomas ◽

Cross Sectional ◽

Borderline Tumors ◽

Epithelial Tumors ◽

Type Iv

Background: the difference in expression of type IV collagen in borderline tumors and ovarian carcinomas has been studied, but the association with adhesion molecules like CD44 have not gain enough interest. Objectives: The purpose of this study is to assess the expression of CD44v6 and type IV collagen status in borderline tumors and invasive ovarian carcinomas and the correlation between them to define the role of these molecules in tumor invasion and metastasis. Type of the study: A cross sectional study Methods: The study included a total of (101) formalin-fixed paraffin-embedded ovarian tissue blocks; of which (19) cases were borderline tumors and (82) cases were overt ovarian carcinomas. Sections from each block were immunohistochemically stained for CD44v6 and type IV collagen. Results: CD44v6 was significantly correlated with FIGO stage of borderline tumors (p=0.001) and ovarian carcinoma (<0.001) and with histological grade of ovarian carcinomas (p=0.004). There was significantly higher expression of type IV collagen in borderline tumors compared to invasive carcinoma(p<0.001) this significance was also seen in correlation to age, stage and grade of the invasive carcinoma, no significant differences in other clinicopathological features were found. There was negative correlation between CD44 v6 & type IV collagen which was statistically significance (P<0.05) in carcinoma but not in borderline tumors. Conclusions: Our data suggest that observed inverse correlation of type IV collagen expression with CD44v6 positivity in surface epithelial tumors indicates that these molecules may cooperate in the invasion and progression of ovarian carcinomas.

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Screening of the residual normal ovarian tissue adjacent to orthotopic epithelial ovarian carcinomas in nude mice

Genetics and Molecular Research ◽

10.4238/2014.april.16.6 ◽

2014 ◽

Vol 13 (2) ◽

pp. 2978-2986 ◽

Cited By ~ 1

Author(s):

G.H. Zhu ◽

S.T. Wang ◽

M.Z. Yao ◽

J.H. Cai ◽

C.Y. Chen ◽

...

Keyword(s):

Nude Mice ◽

Ovarian Tissue ◽

Ovarian Carcinomas ◽

Normal Ovarian Tissue ◽

Epithelial Ovarian Carcinomas

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Ultrasound features and clinical outcome of patients with malignant ovarian masses diagnosed during pregnancy: experience of a gynecological oncology ultrasound center

International Journal of Gynecological Cancer ◽

10.1136/ijgc-2019-000373 ◽

2019 ◽

Vol 29 (7) ◽

pp. 1182-1194

Author(s):

Francesca Moro ◽

Floriana Mascilini ◽

Tina Pasciuto ◽

Martina Leombroni ◽

Marta Li Destri ◽

...

Keyword(s):

Cesarean Section ◽

Metastatic Tumor ◽

Borderline Tumor ◽

Continuous Variables ◽

Ovarian Carcinomas ◽

Borderline Tumors ◽

Operative Ultrasound ◽

The Masses ◽

Ovarian Masses ◽

Epithelial Ovarian Carcinomas

ObjectiveThe number of women diagnosed with ovarian masses during pregnancy has increased in recent years and the management of these women can be controversial. We aim to describe ultrasound characteristics and clinical outcomes of patients with malignant ovarian masses diagnosed during pregnancy.MethodsPatients with a histological diagnosis of malignant ovarian mass detected during pregnancy who underwent pre-operative ultrasound by experienced ultrasound examiners between December 2000 and November 2017 were included in this retrospective observational study. Ultrasound characteristics of the masses were described using International Ovarian Tumor Analysis terminology. Patients with ovarian masses but without histopathological reports were excluded. Results are presented as absolute frequency (percentage) for nominal variables and as median (range) for continuous variables.Results A total of 22 patients were included in the analysis. The median age was 32.5 (range 23–42) years and median gestational age at diagnosis was 13.5 (range 4–30) weeks. Eight (36.4%) patients had a serous/endocervical-type borderline tumor, seven (31.8%) patients had a primary epithelial ovarian carcinoma, five (22.8%) patients had a metastatic tumor to the ovary, and two (9%) patients had a mucinous borderline tumor. At ultrasound, mucinous borderline tumors were multilocular (1/2, 50%) or multilocular-solid (1/2, 50%) lesions. Serous/endocervical-type borderline tumors were unilocular-solid (3/8, 37.5%) or multilocular-solid (5/8, 62.5%) masses and all had papillary projections. Most invasive epithelial ovarian cancers were multilocular-solid masses (5/7, 71.4%). All metastatic tumors appeared as solid masses. No patients with borderline tumors had a cesarean section due to disease, whereas most patients with epithelial ovarian carcinomas (4/7, 57.2%) and with ovarian metastases (3/5, 60%) had a cesarean section due to disease. No neonatal complication was reported for patients with borderline tumors or epithelial ovarian carcinomas, whereas two of three newborns of patients with metastatic tumor died of the disease.ConclusionAt ultrasound, morphological features of malignant ovarian masses detected during pregnancy are similar to those described in non-pregnant patients. The likelihood of undergoing cesarean section increases with malignant disease in the ovary.

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