scholarly journals Fractionation of Volatile Constituents fromCurcumaRhizome by Preparative Gas Chromatography

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
F. Q. Yang ◽  
H. K. Wang ◽  
H. Chen ◽  
J. D. Chen ◽  
Z. N. Xia
Keyword(s):  
Gas Chromatography ◽  
Methanol Extract ◽  
Volatile Components ◽  
Gas Flows ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Fraction Collector ◽  
Preparative Gas Chromatography ◽  
Steel Column ◽  
Stainless Steel Column

A preparative gas chromatography (pGC) method was developed for the separation of volatile components from the methanol extract ofCurcumarhizome. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then, the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% were directed to the fraction collector. Five volatile compounds were collected from the methanol extract ofCurcumarhizome (5 g/mL) after 83 single injections (20 uL) with the yield of 5.1–46.2 mg. Furthermore, the structures of the obtained compounds were identified asβ-elemene, curzerene, curzerenone, curcumenol, and curcumenone by MS and NMR spectra, respectively.

scholarly journals Separation ofcis- andtrans-Asarone fromAcorus tatarinowiiby Preparative Gas Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
H. L. Zuo ◽  
F. Q. Yang ◽  
X. M. Zhang ◽  
Z. N. Xia
Keyword(s):  
Gas Chromatography ◽  
Gradient Elution ◽  
Gel Chromatography ◽  
Gas Flows ◽  
Flame Ionization ◽  
Fraction Collector ◽  
Preparative Gas Chromatography ◽  
Separation Of Isomers ◽  
Stainless Steel Column ◽  
Silica Gel Chromatography

A preparative gas chromatography (pGC) method was developed for the separation of isomers (cis- andtrans-asarone) from essential oil ofAcorus tatarinowii. The oil was primarily fractionated by silica gel chromatography using different ratios of petroleum ether and ethyl acetate as gradient elution solvents. And then the fraction that contains mixture of the isomers was further separated by pGC. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% was directed to the fraction collector. Two isomers were collected after 90 single injections (5 uL) with the yield of 178 mg and 82 mg, respectively. Furthermore, the structures of the obtained compounds were identified ascis- andtrans-asarone by1H- and13C-NMR spectra, respectively.

Gas Chromatographic Determination of Pyrrolidonecarboxylic Acid

1970 ◽  
Vol 53 (4) ◽  
pp. 716-719
Author(s):  
C Y Lee
Keyword(s):  
Stainless Steel ◽  
Analytical Method ◽  
Chromatographic Determination ◽  
Gas Liquid Chromatography ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Extraction Apparatus ◽  
Steel Column ◽  
Stainless Steel Column

Abstract An analytical method is described for analysis of 2-pyrroIidone-5-carboxylic acid (PCA) in foods. PCA was extracted with ethyl acetate, using a modified Widmark extraction apparatus, and then silylated with trimethylchlorosilane and hexamethyldisilazane in the presence of pyridine. The trimethylsilylated PCA derivative was separated by gas-liquid chromatography on a 6′ × 1/4″ °d stainless steel column of 3% SE-30 on Gas Chrom Q and detected with a flame ionization detector. Recovery of PCA was 94-108%. This procedure is a relatively simple and rapid analytical method as compared to the conventional methods.

Identification of Methyl Ester Content from Waste Cooking Oil Using Gas Chromatographic Method

2014 ◽  
Vol 660 ◽  
pp. 297-300
Author(s):  
Nor Hazwani Abdullah ◽  
Sulaiman Hassan
Keyword(s):  
Gas Chromatography ◽  
Methyl Ester ◽  
Acid Methyl Ester ◽  
Waste Cooking Oil ◽  
Molar Ratio ◽  
Ionization Detector ◽  
Cooking Oil ◽  
Ester Content ◽  
Flame Ionization ◽  
Acid Methyl

Waste cooking oil has always been an environment problem in food factories and one method of effect disposing this oil without effecting the environment is to convert it to fatty acid methyl ester (FAME) using small scale pilot plant. The conversion of waste cooking oil with sodium hydroxide as a catalyst in conversional process at 22kHz speed. The reaction of time, molar ratio, speed, catalyst and amount of catalyst will be effect in FAME quality. The quality of biodiesel define is total ester content using gas chromatography. Gas chromatography analysis is a one of technique for identification and quantitation of compounds in a biodiesel sample. From biodiesel sample can identification of contaminants and fatty acid methyl ester. In this research biodiesel sample were analyses using a gas chromatography-flame ionization detector ( Perkin Elmer GC Model Clarus 500) equipped with a DB-5 HT capillary column ( 0.53mm x 5 m) J&W Scientific. The analytic conditions for ester content were as follow by: column temperature used 2100C, temperature flame ionization detector (FID) of 2500C, pressure of 80kPa, flow carrier gas of 1ml/min, temperature injector of 2500C, split flow rate of 50ml/min, time for analysis 20 minute and volume injected of 1 μl. The ester content (C), expresses as a mass fraction in present using formula (EN 14103, 2003a) calculation. Conversion of triglyceride (TG) to FAME using conversional process obtained 96.54 % w.t with methanol to oil molar ratio 6:1, 1%w.t acid sulphuric and 1% w.t sodium hydroxide catalyst.

Composition of N – Alkanes and Polynuclear Aromatic Hydrocarbons in Jatropha curcas Seed Oil from Different Locations

2013 ◽  
Vol 824 ◽  
pp. 311-317
Author(s):  
E.T. Akhihiero ◽  
E.O. Aluyor ◽  
T.O.K. Audu
Keyword(s):  
Gas Chromatography ◽  
Aromatic Hydrocarbons ◽  
Jatropha Curcas ◽  
Seed Oil ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Polynuclear Aromatic Hydrocarbon ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Hydrocarbon Content ◽  
Edo State

The hydrocarbon content of Jatropha curcas seed oil obtained from Oleh Community in Delta State, NIFOR farm in Edo State and Ikabigbo in Edo State represented as samples X, Y and Z respectively were investigated using Gas Chromatography with flame ionization detector. The predominant alkanes found in the oil samples are n-Eicosane (C20H42) and n-Docosane (C22H46), while the major polynuclear aromatic hydrocarbon found in the oil samples is chrysene. Sample X has the highest percentage of n-Eicosane (65.72%), sample Y has 35.56% n-Eicosane while sample Z has the lowest n-Eicosane (0.23%). Sample Z has the highest percentage of n-Docosane (91.38%), sample Y has 60.11% n-Docosane with sample X being the lowest (22.65%). The percentage of chrysene in sample Y is highest (100%), sample X has 97.47% while sample Z has the lowest percentage of chrysene (44.75%).

Sign in / Sign up

Export Citation Format

Share Document