scholarly journals Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage

2012 ◽  
Vol 2012 ◽  
pp. 1-8
Author(s):  
Tammy Tamayo ◽  
Liliana Grajales ◽  
Jesús García
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Calcium Channel ◽  
Satellite Cells ◽  
Cell Attachment ◽  
Donor Cell ◽  
Adult Skeletal Muscle ◽  
Voltage Dependent ◽  
Cell Transplants ◽  
And Migration

Satellite cells can maintain or repair muscle because they possess stem cell properties, making them a valuable option for cell therapy. However, cell transplants into skeletal muscle of patients with muscular dystrophy are limited by donor cell attachment, migration, and survival in the host tissue. Cells used for therapy are selected based on specific markers present in the plasma membrane. Although many markers have been identified, there is a need to find a marker that is expressed at different states in satellite cells, activated, quiescent, or differentiated cell. Furthermore, the marker has to be present in human tissue. Recently we reported that the plasma membrane α2δ1 protein is involved in cell attachment and migration in myoblasts. The α2δ1 subunit forms a part of the L-type voltage-dependent calcium channel in adult skeletal muscle. We found that the α2δ1 subunit is expressed in the majority of newly isolated satellite cells and that it appears earlier than the α1 subunits and at higher levels than the β or γ subunits. We also found that those cells that expressed α2δ1 would differentiate into muscle cells. This evidence indicates that the α2δ1 may be used as a marker of satellite cells that will differentiate into muscle.

The expression patterns of Pax7 in satellite cells during overload-induced rat adult skeletal muscle hypertrophy

2009 ◽  
Vol 195 (4) ◽  
pp. 459-469 ◽  
Author(s):  
M. Ishido ◽  
M. Uda ◽  
N. Kasuga ◽  
M. Masuhara
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Hypertrophy ◽  
Expression Patterns ◽  
Adult Skeletal Muscle ◽  
Skeletal Muscle Hypertrophy

Acidic motif responsible for plasma membrane association of the voltage-dependent calcium channel β1b subunit

2000 ◽  
Vol 12 (3) ◽  
pp. 894-902 ◽  
Author(s):  
Y. Bogdanov ◽  
N. L. Brice ◽  
C. Canti ◽  
K. M. Page ◽  
M. Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Calcium Channel ◽  
Membrane Association ◽  
Voltage Dependent Calcium Channel ◽  
Voltage Dependent

scholarly journals Depletion of resident muscle stem cells negatively impacts running volume, physical function, and muscle fiber hypertrophy in response to lifelong physical activity

2020 ◽  
Vol 318 (6) ◽  
pp. C1178-C1188 ◽  
Author(s):  
Davis A. Englund ◽  
Kevin A. Murach ◽  
Cory M. Dungan ◽  
Vandré C. Figueiredo ◽  
Ivan J. Vechetti ◽  
...  
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Physical Function ◽  
Satellite Cell ◽  
Muscle Fiber ◽  
Satellite Cells ◽  
Cell Depletion ◽  
Muscle Adaptation ◽  
Adult Skeletal Muscle

To date, studies that have aimed to investigate the role of satellite cells during adult skeletal muscle adaptation and hypertrophy have utilized a nontranslational stimulus and/or have been performed over a relatively short time frame. Although it has been shown that satellite cell depletion throughout adulthood does not drive skeletal muscle loss in sedentary mice, it remains unknown how satellite cells participate in skeletal muscle adaptation to long-term physical activity. The current study was designed to determine whether reduced satellite cell content throughout adulthood would influence the transcriptome-wide response to physical activity and diminish the adaptive response of skeletal muscle. We administered vehicle or tamoxifen to adult Pax7-diphtheria toxin A (DTA) mice to deplete satellite cells and assigned them to sedentary or wheel-running conditions for 13 mo. Satellite cell depletion throughout adulthood reduced balance and coordination, overall running volume, and the size of muscle proprioceptors (spindle fibers). Furthermore, satellite cell participation was necessary for optimal muscle fiber hypertrophy but not adaptations in fiber type distribution in response to lifelong physical activity. Transcriptome-wide analysis of the plantaris and soleus revealed that satellite cell function is muscle type specific; satellite cell-dependent myonuclear accretion was apparent in oxidative muscles, whereas initiation of G protein-coupled receptor (GPCR) signaling in the glycolytic plantaris may require satellite cells to induce optimal adaptations to long-term physical activity. These findings suggest that satellite cells play a role in preserving physical function during aging and influence muscle adaptation during sustained periods of physical activity.

scholarly journals 19-Residue Peptide from C-Terminal Tail of DHPR β1A Subunit Potentiates Voltage-Dependent Calcium Release in Adult Skeletal Muscle Fibers

2012 ◽  
Vol 102 (3) ◽  
pp. 362a ◽  
Author(s):  
Rotimi O. Olojo ◽  
Erick O. Hernandez-Ochoa ◽  
Martin F. Schneider
Keyword(s):  
Skeletal Muscle ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Adult Skeletal Muscle ◽  
Skeletal Muscle Fibers ◽  
Voltage Dependent

scholarly journals Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration

Development ◽  
2011 ◽  
Vol 138 (19) ◽  
pp. 4333-4333 ◽  
Author(s):  
R. Sambasivan ◽  
R. Yao ◽  
A. Kissenpfennig ◽  
L. Van Wittenberghe ◽  
A. Paldi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Adult Skeletal Muscle

scholarly journals Skeletal Myogenic Progenitors Originating from Embryonic Dorsal Aorta Coexpress Endothelial and Myogenic Markers and Contribute to Postnatal Muscle Growth and Regeneration

1999 ◽  
Vol 147 (4) ◽  
pp. 869-878 ◽  
Author(s):  
Luciana De Angelis ◽  
Libera Berghella ◽  
Marcello Coletta ◽  
Laura Lattanzi ◽  
Malvina Zanchi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Developmental Stages ◽  
Vascular System ◽  
Muscle Growth ◽  
Paraxial Mesoderm ◽  
Dorsal Aorta ◽  
Myogenic Cells ◽  
Adult Skeletal Muscle

Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met−/− embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.

scholarly journals Pax7 is critical for the normal function of satellite cells in adult skeletal muscle

2013 ◽  
Vol 110 (41) ◽  
pp. 16474-16479 ◽  
Author(s):  
J. von Maltzahn ◽  
A. E. Jones ◽  
R. J. Parks ◽  
M. A. Rudnicki
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Normal Function ◽  
Adult Skeletal Muscle

In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle

1999 ◽  
Vol 112 (17) ◽  
pp. 2895-2901 ◽  
Author(s):  
R.N. Cooper ◽  
S. Tajbakhsh ◽  
V. Mouly ◽  
G. Cossu ◽  
M. Buckingham ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Cell Activation ◽  
Muscle Fibres ◽  
Adult Skeletal Muscle ◽  
Satellite Cell Activation ◽  
Slow Muscle Fibres ◽  
Myogenic Factors ◽  
Beta Galactosidase

Regeneration of adult skeletal muscle is an asynchronous process requiring the activation, proliferation and fusion of satellite cells, to form new muscle fibres. This study was designed to determine the pattern of expression in vivo of the two myogenic regulatory factors, Myf5 and MyoD during this process. Cardiotoxin was used to induce regeneration in the gastrocnemius and soleus muscles of heterozygous Myf5-nlacZ mice, and the muscles were assayed for the presence of (beta)-galactosidase (Myf5) and MyoD. Adult satellite cells identified by M-cadherin labelling, when activated, initially express either MyoD or Myf5 or both myogenic factors. Subsequently all proliferating myoblasts express MyoD and part of the population is (beta)-galactosidase (Myf5) positive. Furthermore, we demonstrate that activated satellite cells, which express either Myf5 or MyoD, do not accumulate selectively on fast or slow muscle fibres.

Tristetraprolin and LPS-inducible CXC chemokine are rapidly induced in presumptive satellite cells in response to skeletal muscle injury

2002 ◽  
Vol 115 (13) ◽  
pp. 2701-2712 ◽  
Author(s):  
Chetana Sachidanandan ◽  
Ramkumar Sambasivan ◽  
Jyotsna Dhawan
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Cell Biology ◽  
Muscle Injury ◽  
Rna Binding ◽  
C2c12 Myoblasts ◽  
Adult Skeletal Muscle ◽  
Cxc Chemokine

Myogenic precursor cells known as satellite cells persist in adult skeletal muscle and are responsible for its ability to regenerate after injury. Quiescent satellite cells are activated by signals emanating from damaged muscle. Here we describe the rapid activation of two genes in response to muscle injury; these transcripts encode LPS-inducible CXC chemokine (LIX), a neutrophil chemoattractant, and Tristetraprolin (TTP), an RNA-binding protein implicated in the regulation of cytokine expression. Using a synchronized cell culture model we show that C2C12 myoblasts arrested in G0 exhibit some molecular attributes of satellite cells in vivo: suppression of MyoD and Myf5 expression during G0 and their reactivation in G1. Synchronization also revealed cell cycle dependent expression of CD34, M-cadherin, HGF and PEA3, genes implicated in satellite cell biology. To identify other genes induced in synchronized C2C12 myoblasts we used differential display PCR and isolated LIX and TTP cDNAs. Both LIX and TTP mRNAs are short-lived, encode molecules implicated in inflammation and are transiently induced during growth activation in vitro. Further, LIX and TTP are rapidly induced in response to muscle damage in vivo. TTP expression precedes that of MyoD and is detected 30 minutes after injury. The spatial distribution of LIX and TTP transcripts in injured muscle suggests expression by satellite cells. Our studies suggest that in addition to generating new cells for repair, activated satellite cells may be a source of signaling molecules involved in tissue remodeling during regeneration.

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