scholarly journals Effect of Electroacupuncture on Activation of p38MAPK in Spinal Dorsal Horn in Rats with Complete Freund's Adjuvant-Induced Inflammatory Pain

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Yi Liang ◽  
Jian-Qiao Fang ◽  
Jun-Ying Du ◽  
Jun-Fan Fang
Keyword(s):  
P38 Mapk ◽  
Inflammatory Pain ◽  
Saline Group ◽  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Intraplantar Injection ◽  
Male Adult ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Activation of mitogen-activated protein kinases (MAPKs), especially p38 MAPK, plays an important role in the development of central sensitization related to persistent inflammatory pain. Electroacupuncture (EA) is well known to relieve persistent inflammatory pain. However, little is known about relationship between EA and p38 MAPK. Inflammatory pain rat model was induced by intraplantar injection of complete Freund's adjuvant (CFA). Male adult SD rats were randomly divided into the saline group, CFA group, and CFA + EA group. EA (constant saquare wave, 2 Hz and 100 Hz alternating frequencies, intensities ranging from 1 to 2 mA) was applied to bilateral “Zusanli” (ST 36) and “Kunlun” acupoints (BL 60) for 30 min, once per day. The paw edema and paw withdrawal threshold (PWT) were measured at preinjection and days postinjection 1, 3, and 14. Spinal p-p38MAPK- immunoreactivty (p-p38MAPK-IR) cells were detected by immunohistochemistry at postinjection day 3 and 14. EA significantly inhibited paw edema at postinjection days 14 and increased PWT at postinjection days 3 and 14. Moreover, the increasing number of spinal p-p38MAPK-IR cells which was induced by CFA injection was suppressed by EA stimulation. These results indicate that anti-inflammatory and analgesic effect of EA might be associated with its inhibition of spinal p38 MAPK activation and thereby provide a potential mechanism for the treatment of inflammatory pain by EA.

Behavioral outcomes of complete Freund’s adjuvant-induced inflammatory pain in the rodent hind-paw

Pain ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Dominika J. Burek ◽  
Nicolas Massaly ◽  
Hye Jean Yoon ◽  
Michelle Doering ◽  
Jose A. Morón
Keyword(s):  
Inflammatory Pain ◽  
Behavioral Outcomes ◽  
Complete Freund’S Adjuvant ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Effects of Electroacupuncture with Different Frequencies on the Glycoconjugate Alterations in Articular Cartilage in the Ankle Joints of Complete Freund's Adjuvant-injected Rats

2006 ◽  
Vol 34 (03) ◽  
pp. 417-426 ◽  
Author(s):  
In-Bum Park ◽  
Chang-Beohm Ahn ◽  
Byung-Tae Choi
Keyword(s):  
Articular Cartilage ◽  
Experimental Period ◽  
Lectin Histochemistry ◽  
Complete Freund’S Adjuvant ◽  
Sprague Dawley ◽  
Water Displacement ◽  
Intraplantar Injection ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

The aim of this study was to investigate the effects of electroacupuncture (EA) on the glycoconjugate (GC) changes in articular cartilage in the ankle of an arthritic model. Arthritis was induced by an intraplantar injection of complete Freund's adjuvant (CFA) into the hindpaw of male Sprague-Dawley rats. Bilateral EA stimulation at 2 Hz, 15 Hz and 120 Hz was applied at those acupoints corresponding to Zusanli and Sanyinjiao in man, using needles for 3-day intervals for 30 days. To determine the presence of arthritis, paw edema was measured by a water displacement plethysmometer. Edema of the hindpaw induced by CFA-injection was strongly inhibited by EA stimulation throughout the experimental period. At 30 days after CFA-injection, GC changes of articular cartilage of the ankle joint were observed using conventional and lectin histochemistry. The CFA-injected rat revealed general reduction of staining abilities and lectin affinities for GC in comparison with normal rats. Significant reductions of neutral and acidic GC were observed in interterritorial matrix and chondrocyte capsules, respectively. All lectin affinities examined except DBA were also decreased in CFA-injected rats compared to normal ones. However, EA-treated rats, showed similar staining patterns and lectin affinities for GC as to normal ones, especially neutral GC in interterritorial matrix and sWGA and RCA-1 affinities in chondrocytes. It is concluded that EA in all frequencies examined, especially 2 Hz, can attenuate inflammatory edema in CFA-injected rats through alleviation of alterations of GC components in articular cartilage.

scholarly journals VGLUT2/Cdk5/p25 Signaling Pathway Contributed to Inflammatory Pain by Complete Freund’s Adjuvant

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yuwen Tang ◽  
Zhiyou Peng ◽  
Shoujun Tao ◽  
Jianliang Sun ◽  
Wenyuan Wang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Signaling Pathway ◽  
Inflammatory Pain ◽  
Glutamate Transporter ◽  
Underlying Mechanism ◽  
Complete Freund’S Adjuvant ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Heat Hyperalgesia

Vesicular glutamate transporter type 2 (VGLUT2) is known to play an important role in mediating heat hyperalgesia induced by inflammation. However, the underlying mechanism for this activity is poorly understood. Cyclin-dependent kinase 5 (Cdk5), serving as a key regulator in modulating release of glutamate, acted a key player in the formation of heat hyperalgesia of inflammatory pain. However, it remains unknown whether there is a bridge between Cdk5 and VGLUT2 for mediating inflammatory pain. Therefore, we designed the experiment to determine whether VGLUT2 signaling pathway is involved in inflammatory pain mediated by Cdk5 in the inflammatory pain model induced by complete Freund’s adjuvant (CFA). Our results showed that the coexpression of Cdk5/VGLUT2 in small- and medium-sized neuronal cells of the dorsal root ganglion (DRG) and spinal cord between days 1 and 3 following subcutaneous injection of CFA was significantly increased. Moreover, our study revealed that the expression of VGLUT2 protein in the DRG and spinal cord was remarkably increased between days 1 and 3 following CFA injection and was significantly reduced by roscovitine, a selective antagonist of Cdk5. Additionally, p25 but not p35, an activator of Cdk5, protein was significantly increased by CFA and reduced by roscovitine. Our findings suggested that VGLUT2/Cdk5 signaling pathway contributes to inflammatory pain mediated by Cdk5/p25.

Anti-inflammatory Activity of Taiwan Folk Medicine "Ham-Hong-Chho" in Rats

1995 ◽  
Vol 23 (03n04) ◽  
pp. 273-278 ◽  
Author(s):  
Hwa-Woei Chih ◽  
Chun-Ching Lin ◽  
Kung-Sheng Tang
Keyword(s):  
Folk Medicine ◽  
Chronic Arthritis ◽  
Complete Freund’S Adjuvant ◽  
Aqueous Extracts ◽  
Paw Edema ◽  
Bidens Pilosa ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory

"Ham-Hong-Chho" is a folk medicine in Taiwan, derived from the entire plants of Bidens pilosa L. var. minor (Blume) Sherff (Compositae), B. pilosa L. and B. chilensis DC. The anti-inflammatory effect of aqueous extracts of the three plants against paw edema induced by carrageenan and chronic arthritis induced by complete Freund's adjuvant were determined in rats. The results indicated that paw edema induced by carrageenan was significantly decreased by treatment with aqueous extracts (150 or 300 mg/kg) of all three plants ( p < 0.05) and that the effect of Bidens pilosa var. minor was the most potent. However, only extracts (500 mg/kg) of B. pilosa L. var. minor and B.pilosa L. significantly decreased the paw edema induced by complete Freund's adjuvant ( p < 0.05).

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