scholarly journals The Spectrophotometric Multicomponent Analysis of a Ternary Mixture of Paracetamol, Aspirin, and Caffeine by the Double Divisor-Ratio Spectra Derivative Method

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Reza Hajian ◽  
Ahmad Soltaninezhad
Keyword(s):  
Absorption Spectrum ◽  
Ternary Mixture ◽  
Simultaneous Analysis ◽  
Mathematical Explanation ◽  
Multicomponent Analysis ◽  
Ratio Spectrum ◽  
Standard Spectrum ◽  
Derivative Method ◽  
Spectrophotometric Data

Double divisor-ratio spectra derivative method based on the spectrophotometric data was developed for the simultaneous analysis of a ternary mixture containing paracetamol, aspirin, and caffeine, without prior separation. This method is based on the use of the derivative of the ratio spectrum obtained by dividing the absorption spectrum of the ternary mixture by a standard spectrum of a mixture of two of the three compounds in the title mixture. The concentrations of three compounds in their mixture are determined by using their respective calibration graphs which are obtained by measuring the amplitude at either the maximum or minimum wavelengths selected. The selected wavelengths for determination of aspirin, caffeine, and paracetamol are 241.5, 256, and 258.5 nm, respectively. All of the solutions adjusted to pH 11 before recording the spectra in the range of 220 to 320 nm. Also, the mathematical explanation of the procedure is illustrated. The method was applied for the assay of Excedrin containing paracetamol, aspirin, and caffeine.

scholarly journals The Spectrophotometric Multicomponent Analysis of a Ternary Mixture of Ibuprofen, Caffeine and Paracetamol by the Combination of Double Divisor-Ratio Spectra Derivative and H-Point Standard Addition Method

2012 ◽  
Vol 9 (3) ◽  
pp. 1153-1164 ◽  
Author(s):  
R. Hajian ◽  
N. Afshari
Keyword(s):  
Ternary Mixture ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Mathematical Explanation ◽  
Addition Method ◽  
Alternative Analysis ◽  
Ratio Spectrum ◽  
Standard Spectrum ◽  
Study Results ◽  
Recovery Study

A new spectrophotometric method was developed for the simultaneous analysis of a ternary mixture containing paracetamol (PAR), ibuprofen (IBU) and caffeine (CAF) without prior separation. H-point standard addition method (HPSAM) was used for the first time in the analysis of a component (IBU) in a ternary mixture (paracetamol, ibuprofen and caffeine). In contrast, PAR and CAF determined using double divisor ratio spectra derivative method. This method is based on the use of derivative of the ratio spectrum obtained by dividing the absorption spectrum of the ternary mixture by a standard spectrum of a mixture of two of the three compounds in the title mixture. The concentrations of PAR and CAF compounds in their mixture are determined by using their respective standard addition graphs which are obtained by measuring the amplitude at either the maximum or minimum wavelengths selected. The mathematical explanation of the procedure is illustrated. It was shown that at wavelengths 226 and 260 nm, the coordinate of H-point is only dependent on the concentration of IBU without any interference by PAR and CAF. This method was successfully applied for the analysis of Novafen capsule, with no interference from excipients as indicated by the recovery study results. The proposed method is simple and rapid and can be easily used in the quality control of drugs as an alternative analysis tools.

scholarly journals Simultaneous Determination of Amlodipine and Valsartan

2011 ◽  
Vol 6 ◽  
pp. ACI.S7282 ◽  
Author(s):  
Nashwah Gadallah Mohamed
Keyword(s):  
Absorption Spectrum ◽  
Simultaneous Determination ◽  
Zero Order ◽  
Uv Spectrophotometry ◽  
Ratio Spectrum ◽  
First Derivative ◽  
Bulk Powder ◽  
Overlapped Peaks ◽  
Third Derivative

A spectrophotometric method was developed for simultaneous determination of amlodipine (Aml) and valsartan (Val) without previous separation. In this method amlodipine in methanolic solution was determined using zero order UV spectrophotometry by measuring its absorbency at 360.5 nm without any interference from valsartan. Valsartan spectrum in zero order is totally overlapped with that of amlodipine. First, second and third derivative could not resolve the overlapped peaks. The first derivative of the ratio spectra technique was applied for the measurement of valsartan. The ratio spectrum was obtained by dividing the absorption spectrum of the mixture by that of amlodipine, so that the concentration of valsartan could be determined from the first derivative of the ratio spectrum at 290 nm. Quantification limits of amlodipine and valsartan were 10-80 μg/ml and 20-180 μg/ml respectively. The method was successfully applied for the quantitative determination of both drugs in bulk powder and pharmaceutical formulation.

Simultaneous analysis of three catecholamines by a kinetic procedure: comparison of prediction performance of several different multivariate calibrations

2011 ◽  
Vol 65 (6) ◽  
Author(s):  
Yongnian Ni ◽  
Yao Gu ◽  
Serge Kokot
Keyword(s):  
High Performance ◽  
Multivariate Calibration ◽  
Kinetic Method ◽  
Simultaneous Analysis ◽  
Rabbit Serum ◽  
Serum Samples ◽  
Consecutive Reaction ◽  
Calibration Models ◽  
Spectrophotometric Data

AbstractA rapid kinetic method for the simultaneous determination of levodopa, dopamine, and dobutamine was examined and developed. It was based on a consecutive reaction of a reduction of Cu(II) to Cu(I) by catecholamines, followed by the complexation of Cu(I) with neocuproine to form a yellow product in an acetic acid-acetate buffer. Spectrophotometric data were recorded at 453 nm (wavelength at the yellow complex absorption maximum) for 300 s. Linear calibrations were obtained in the concentration ranges of (0.08–1.44) × 10−5 mol L−1, (0.08–1.44) × 10−5 mol L−1, and (0.16–1.44) × 10−5 mol L−1 for levodopa, dopamine, and dobutamine, respectively. A variety of multivariate calibration models was developed for simultaneous analysis of the three analytes; while most models produced satisfactory prediction results for synthetic samples, the hybrid linear analysis method was arguably the best-performing (relative prediction error, RPET = 6.6 %). The proposed method was applied to an analysis of spiked rabbit serum samples and the results showed good agreement with the high performance liquid chromatography measurements.

scholarly journals Determination of antihypertensive drugs by using ratio difference spectrophotometric method

2019 ◽  
Vol 10 (1) ◽  
pp. 12-18
Author(s):  
Nessreen Salah Abdelhamid ◽  
Eglal Abdelhamid Abd El Aleem El Aleem ◽  
Aml Mohamed Khorshed ◽  
Mahmoud Mohsen Amin
Keyword(s):  
Spectrophotometric Method ◽  
Anthranilic Acid ◽  
Ternary Mixture ◽  
Pharmaceutical Preparations ◽  
The Other ◽  
Ratio Spectrum ◽  
Other Hand ◽  
Significant Difference ◽  
The Difference

An accurate, sensitive and time saving spectrophotometric method has been developed and validated for the determination of two antihypertensive drug mixtures. Mixture 1 contains spironolactone (SPIR), furosemide (FUR) and anthranilic acid (ANTH) (impurity of furosemide) and mixture 2 contains triamterene (TRI), hydrochlorothiazide (HCZ) and chlorothiazide (CZ) (impurity of hydrochlorothiazide). In mixture 1, the determination of drugs depends on dividing the spectrum of ternary mixture by the spectrum of 10 µg/mL of standard furosemide and then spironolactone and anthranilic acid were determined using the difference in amplitude between 242.3 and 254.6 nm, and between 250.8 and 242.4 nm in the ratio spectrum, respectively. On the other hand, furosemide could be determined by dividing the spectrum of ternary mixture by the spectrum of 10 µg/mL of standard spironolactone and then it was determined using the difference in amplitude between 244.8 and 229.7 nm in the ratio spectrum. In mixture 2, the determination of drugs depends on dividing the spectrum of ternary mixture by spectrum of 10 µg/mL of standard triamterene and then hydrochlorothiazide and chlorothiazide were determined using the difference in amplitude between 268.9 and 232.8 nm, and between 292.9 and 250.7 nm in the ratio spectrum, respectively. On the other hand, triamterene could be determined by dividing spectrum of ternary mixture by spectrum of 10 µg/mL of standard hydrochlorothiazide and then triamterene was determined using the difference in amplitude between 230.1 and 244 nm in the ratio spectrum. The developed analytical methods were validated regarding good accuracy and precision according to The International Conference on Harmonisation guidelines, and they were applied to pharmaceutical preparations in addition to laboratory prepared mixtures successfully. Statistically the results were compared with those obtained by reported method and no significant difference was found.

scholarly journals SIMULTANEOUS DETERMINATION OF PARACETAMOL AND TAPENTADOL IN TABLETS BY RATIO SPECTRA DERIVATIVE SPECTROPHOTOMETRY

2021 ◽  
Vol 12 (9) ◽  
pp. 27-32
Author(s):  
Shah Esha Bhavin ◽  
Gajjar Anuradha
Keyword(s):  
Absorption Spectrum ◽  
Binary Mixture ◽  
Simultaneous Determination ◽  
Derivative Spectrophotometry ◽  
Pharmaceutical Tablets ◽  
First Derivative ◽  
Standard Spectrum ◽  
Ich Guidelines ◽  
Relative Standard

The application of the ratio spectra derivative spectrophotometry to the simultaneous determination of Paracetamol (PCM) and Tapentadol (TAP) in combined pharmaceutical tablets is presented. The spectrophotometric procedure is based on the use of the first derivative of the ratio spectra obtained by dividing the absorption spectrum of the binary mixtures by a standard spectrum of one of the compounds. The first derivative amplitudes were measured at 220 and 232 nm for the assay of TAP and PCM, respectively. Calibration graphs were established for 1-5 μg mL-1 for TAP and 6.5-32.5 μg mL-1 for PCM in binary mixture. The detection limits for TAP and PCM were found 0.098 and 0.595 μg mL-1, respectively, while the quantification limits were 0.298 μg mL-1 for TAP and 1.805 μg/ml for PCM. The relative standard deviations were found to be less than 2%, indicating reasonable repeatability of both methods. The proposed methods were hence validated as per ICH guidelines and successfully applied to the determination of these drugs in commercial tablets.

Simultaneous Determination of Epinephrine and Tyrosine Using a Glassy Carbon Electrode Amplified with ZnO-Pt/CNTs Nanocomposite

2019 ◽  
Vol 15 (2) ◽  
pp. 166-171 ◽  
Author(s):  
Ali Samadzadeh ◽  
Iran Sheikhshoaie ◽  
Hassan Karimi-Maleh
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Simultaneous Analysis ◽  
Urine Samples ◽  
Carbon Electrode ◽  
Linear Dynamic ◽  
Biological Compounds ◽  
Blood And Urine Samples ◽  
Human Blood And Urine

Background: Simultaneous analysis of epinephrine and tyrosine as two effective and important biological compounds in human blood and urine samples are very important for the investigation of human health. Objective: In this research, a highly effective voltammetric sensor fabricated for simultaneous analysis of epinephrine and tyrosine. The sensor was fabricated by the modification of glassy carbon electrode with ZnO-Pt/CNTs nanocomposite (ZnO-Pt/CNTs/GCE). The synthesized nanocomposite was characterized by SEM method. The ZnO-Pt/CNTs/GCE showed two separated oxidation signals at potential ~220 mV and 700 mV for epinephrine and tyrosine, respectively. Also, we detected linear dynamic ranges 0.5-250.0 µM and 1.0-220 µM with a limit of detections 0.1 µM and 0.5 µM for the determination of epinephrine and tyrosine, respectively. The ZnO-Pt/CNTs/GCE was used for the determination of epinephrine and tyrosine in blood serum and human urine samples.

scholarly journals Application of Derivative, Derivative Ratio, and Multivariate Spectral Analysis and Thin-Layer Chomatography-Densitometry for Determination of a Ternary Mixture Containing Drotaverine Hydrochloride, Caffeine, and Paracetamol

2007 ◽  
Vol 90 (2) ◽  
pp. 391-404 ◽  
Author(s):  
Fadia H Metwally ◽  
Yasser S El-Saharty ◽  
Mohamed Refaat ◽  
Sonia Z El-Khateeb
Keyword(s):  
Thin Layer ◽  
Least Squares ◽  
Ternary Mixture ◽  
Principal Component Regression ◽  
Pharmaceutical Preparations ◽  
Principal Component ◽  
Standard Addition ◽  
Drotaverine Hydrochloride ◽  
Assay Precision

Abstract New selective, precise, and accurate methods are described for the determination of a ternary mixture containing drotaverine hydrochloride (I), caffeine (II), and paracetamol (III). The first method uses the first (D1) and third (D3) derivative spectrophotometry at 331 and 315 nm for the determination of (I) and (III), respectively, without interference from (II). The second method depends on the simultaneous use of the first derivative of the ratio spectra (DD1) with measurement at 312.4 nm for determination of (I) using the spectrum of 40 μg/mL (III) as a divisor or measurement at 286.4 and 304 nm after using the spectrum of 4 μg/mL (I) as a divisor for the determination of (II) and (III), respectively. In the third method, the predictive abilities of the classical least-squares, principal component regression, and partial least-squares were examined for the simultaneous determination of the ternary mixture. The last method depends on thin-layer chromatography-densitometry after separation of the mixture on silica gel plates using ethyl acetatechloroformmethanol (16 + 3 + 1, v/v/v) as the mobile phase. The spots were scanned at 281, 272, and 248 nm for the determination of (I), (II), and (III), respectively. Regression analysis showed good correlation in the selected ranges with excellent percentage recoveries. The chemical variables affecting the analytical performance of the methodology were studied and optimized. The methods showed no significant interferences from excipients. Intraday and interday assay precision and accuracy values were within regulatory limits. The suggested procedures were checked using laboratory-prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparations. The validity of the proposed methods was further assessed by applying a standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the manufacturer's method.

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