DNA Detection ofToxoplasma gondiiwith a Magnetic Molecular Beacon Probe via CdTe@Ni Quantum Dots as Energy Donor

A new method for detection ofToxoplasma gondiivia DNA sensing technology was developed in this study. It was based on the mechanism of fluorescence resonance energy transfer (FRET) in which multifunctional and magnetic-fluorescent CdTe@Ni quantum dots (mQDs) were utilized as energy donor and a commercial BHQ2as acceptor. The sensing probe was fabricated by labeling a stem-loopToxoplasma gondiiDNA oligonucleotide with CdTe@Ni mQDs at the 5′ end and BHQ2at 3′ end, respectively. The surface assembly of CdTe on Ni core and the formation of CdTe@Ni were confirmed by XRD analysis. The sizes of CdTe, Ni nanoparticles, and CdTe@Ni were measured via TEM and XRD methods and estimated to be~3 nm,~15 nm, and~20 nm, respectively. The sensing ability was investigated by the fluorescence spectrum (FS). An obvious fluorescence recovery was observed when the complete complimentary targetToxoplasma gondiiDNA was introduced, which did not happen in the case of the target DNA with one-base pair mismatch. Our research indicates that the current sensing probe is sensitive and specific in detection ofToxoplasma gondiiDNA and has great potential in Toxoplasmosis diagnosis.

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Toxoplasma gondii DNA Sensor Based on a Novel Ni-Magnetic Sensing Probe

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.152-153.1510 ◽

2010 ◽

Vol 152-153 ◽

pp. 1510-1513 ◽

Cited By ~ 2

Author(s):

Heng Miao ◽

Shi Chao Xu ◽

Yue Qian Yang ◽

Ji Mei Zhang ◽

Zhao Dai ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

High Sensitivity ◽

Core Material ◽

Ni Nanoparticles ◽

Simple Method ◽

Stem Loop ◽

Current Sensing ◽

Potential Applications

we introduced a fast, specific, and sensitive sensing probe to detect Toxoplasma gondii DNA based on mechanism of fluorescence resonance energy transfer (FRET), and a multifunctional and magnetic-fluorescent CdTe/Ni quantum dots (mQDs) was prepared as energy donor, and BHQ2 was used as energy acceptor, respectively. CdTe/Ni mQDs were synthesized with a more simple method using Ni nanoparticles as core material and CdTe as shell material. The sensing probe was fabricated through labeling a stem-loop Toxoplasma gondii DNA oligonucleotide with CdTe/Ni mQDs at the 5′ end and BHQ2 at 3′ end, respectively, and the resulting sensing probe can be conveniently isolated and purified with a common magnet. Properties of mQDs and sensing probe were investigated by transmission electron microscopy (TEM), X-ray diffraction (XRD), and fluorescence spectrum (FS) methods. The TEM data demonstrated that the size of Ni nanoparticles was estimated to be ~10nm, and size of CdTe/Ni is 15nm. XRD data showed similar spectrum of CdTe and CdTe/Ni, intensity of Ni (111) typical diffraction peak was detected, which inferred the formation of CdTe on surface of Ni core. An obvious fluorescence recovery (FR) was observed when the complete complimentary target Toxoplasma gondii DNA was introduced comparing with the target DNA with one-basepair-mismatch, this result revealed the sensing probe has high sensitivity and specificity. The current sensing probe will has great potential applications in the life science and gene diagnostics.

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High-performance Förster resonance energy transfer-based dye-sensitized photocatalytic H2 evolution with graphene quantum dots as the homogeneous energy donor

Photochemical & Photobiological Sciences ◽

10.1039/c8pp00227d ◽

2018 ◽

Vol 17 (9) ◽

pp. 1147-1152 ◽

Cited By ~ 4

Author(s):

Yonggang Lei ◽

Yuan Xue ◽

Yanan Li ◽

Xiangyu Liu ◽

Fang Wang ◽

...

Keyword(s):

Quantum Dots ◽

Energy Transfer ◽

High Performance ◽

Graphene Quantum Dots ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Evolution System ◽

H2 Evolution ◽

Dye Sensitized ◽

Energy Donor

A highly efficient dye sensitized photocatalytic H2 evolution system based on Förster resonant energy transfer has been developed by employing N,S codoped graphene quantum dots as energy donor.

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