scholarly journals Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off: Morphological Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Jameel Shaik ◽  
Javeed Shaikh Mohammed ◽  
Michael J. McShane ◽  
David K. Mills
Keyword(s):  
Biomedical Applications ◽  
Cellular Response ◽  
Morphological Analysis ◽  
Layer By Layer ◽  
Lab On Chip ◽  
Cell Characterization ◽  
Characterization Studies ◽  
Lift Off ◽  
On Chip ◽  
Poly Styrene Sulfonate

Cell patterning has emerged as an elegant tool in developing cellular arrays, bioreactors, biosensors, and lab-on-chip devices and for use in engineering neotissue for repair or regeneration. In this study, micropatterned surfaces were created using the layer-by-layer lift-off (LbL-LO) method for analyzing canine chondrocytes response to patterned substrates. Five materials were chosen based on our previous studies. These included: poly(dimethyldiallylammonium chloride) (PDDA), poly(ethyleneimine) (PEI), poly(styrene sulfonate) (PSS), collagen, and chondroitin sulfate (CS). The substrates were patterned with these five different materials, in five and ten bilayers, resulting in the following multilayer nanofilm architectures: (PSS/PDDA)5, (PSS/PDDA)10; (CS/PEI)4/CS, (CS/PEI)9/CS; (PSS/PEI)5, (PSS/PEI)10; (PSS/Collagen)5, (PSS/Collagen)10; (PSS/PEI)4/PSS, (PSS/PEI)9/PSS. Cell characterization studies were used to assess the viability, longevity, and cellular response to the configured patterned multilayer architectures. The cumulative cell characterization data suggests that cell viability, longevity, and functionality were enhanced on micropatterned PEI, PSS, collagen, and CS multilayer nanofilms suggesting their possible use in biomedical applications.

Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process

2015 ◽  
Vol 212 (6) ◽  
pp. 1347-1352 ◽  
Author(s):  
S. Takenaga ◽  
B. Schneider ◽  
E. Erbay ◽  
M. Biselli ◽  
Th. Schnitzler ◽  
...  
Keyword(s):  
3D Printing ◽  
Biomedical Applications ◽  
Printing Process ◽  
Lab On Chip ◽  
On Chip

scholarly journals Microoxygraph Device for Biosensoristic Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
A. Aloisi ◽  
E. Tarentini ◽  
A. Ferramosca ◽  
V. Zara ◽  
R. Rinaldi
Keyword(s):  
Response Time ◽  
Low Cost ◽  
Reference Electrode ◽  
Reaction Chamber ◽  
Counter Electrodes ◽  
Permeable Membrane ◽  
Lab On Chip ◽  
Lift Off ◽  
On Chip

Oxygen consumption rate (OCR) is a significant parameter helpful to determinein vitrorespiratory efficiency of living cells. Oxygen is an excellent oxidant and its electrocatalytic reduction on a noble metal allows accurately detecting it. By means of microfabrication technologies, handy, low-cost, and disposable chip can be attained, minimizing working volumes and improving sensitivity and response time. In this respect, here is presented a microoxygraph device (MOD), based on Clark’s electrode principle, displaying many advantageous features in comparison to other systems. This lab-on-chip platform is composed of a three-microelectrode detector equipped with a microgrooved electrochemical cell, sealed with a polymeric reaction chamber. Au working/counter electrodes and Ag/AgCl reference electrode were fabricated on a glass slide. A microchannel was realized by photoresist lift-off technique and a polydimethylsiloxane (PDMS) nanoporous film was integrated as oxygen permeable membrane (OPM) between the probe and the microreaction chamber. Electrochemical measurements showed good reproducibility and average response time, assessed by periodic injection and suction of a reducing agent. OCR measurements on 3T3 cells, subjected, in real time, to chemical stress on the respiratory chain, were able to show that this chip allows performing consistent metabolic analysis.

Label-Free 3D Imaging for Lab-on-Chip Biomedical Applications

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Lisa Miccio ◽  
Francesco Merola ◽  
Pasquale Memmolo ◽  
Pietro Ferraro
Keyword(s):  
Biomedical Applications ◽  
3D Imaging ◽  
Label Free ◽  
Lab On Chip ◽  
On Chip

scholarly journals Lactobacillus acidophilus Derived Biosurfactant as a Biofilm Inhibitor: A Promising Investigation Using Microfluidic Approach

2018 ◽  
Vol 8 (9) ◽  
pp. 1555 ◽  
Author(s):  
Surekha Satpute ◽  
Nishigandha Mone ◽  
Parijat Das ◽  
Arun Banpurkar ◽  
Ibrahim Banat
Keyword(s):  
Lactobacillus Acidophilus ◽  
Biomedical Applications ◽  
Health Issues ◽  
Lab On Chip ◽  
On Chip ◽  
Catheter Tubing ◽  
Pathogenic Biofilms ◽  
Biofilm Inhibitor ◽  
Lactobacillus Spp ◽  
Disc Assay

Background: Biomedical devices and implants are adversely affected by biofilm-associated infections that pose serious public health issues. Biosurfactants (BSs) can combat pathogenic biofilms through their antimicrobial, antibiofilm and antiadhesive capabilities. The objective of our research was to produce biosurfactant (BS) from Lactobacillus acidophilus NCIM 2903 and investigate its antibiofilm, antiadhesive potential using microfluidics strategies by mimicking the micro-environment of biofilm. Methods: Antibiofilm and antiadhesive potential was effectively evaluated using different methods like microfluidics assay, catheter assay, polydimethlysiloxane (PDMS) disc assay. Along with this chemical and physical characteristics of BS were also evaluated. Results: Cell free biosurfactant (CFBS) obtained was found to be effective against biofilm which was validated through the microfluidic (MF) or Lab on Chip (LOC) approach. The potency of CFBS was also evaluated on catheter tubing and PDMS surfaces (representative bioimplants). The efficacy of CFBS was also demonstrated through the reduction in surface tension, interfacial tension, contact angle and low critical micelle concentration. Conclusion: CFBS was found to be a potent antimicrobial and antibiofilm agent. We believe that perhaps this is the first report on demonstrating the inhibiting effect of Lactobacillus spp. derived CFBS against selected bacteria via LOC approach. These findings can be explored to design various BSs based formulations exhibiting antimicrobial, antibiofilm and antiadhesive potential for biomedical applications.

Integrated photonic lab-on-chip systems for biomedical applications

2010 ◽  
Author(s):  
Timo Mappes ◽  
Christoph Vannahme ◽  
Sönke Klinkhammer ◽  
Uwe Bog ◽  
Mauno Schelb ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Lab On Chip ◽  
On Chip

scholarly journals Local Acoustic Fields Powered Assembly of Microparticles and Applications

Micromachines ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 882
Author(s):  
Hui Shen ◽  
Kangdong Zhao ◽  
Zhiwen Wang ◽  
Xiaoyu Xu ◽  
Jiayu Lu ◽  
...  
Keyword(s):  
Acoustic Field ◽  
Biomedical Applications ◽  
Energy Sources ◽  
Driving Frequency ◽  
Lab On Chip ◽  
Assembly Strategy ◽  
On Demand ◽  
Propulsion Force ◽  
On Chip ◽  
Size Of Particles

Controllable assembly in nano-/microscale holds considerable promise for bioengineering, intracellular manipulation, diagnostic sensing, and biomedical applications. However, up to now, micro-/nanoscopic assembly methods are severely limited by the fabrication materials, as well as energy sources to achieve the effective propulsion. In particular, reproductive manipulation and customized structure is quite essential for assemblies to accomplish a variety of on-demand tasks at small scales. Here, we present an attractive assembly strategy to collect microparticles, based on local acoustic forces nearby microstructures. The micro-manipulation chip is built based on an enhanced acoustic field, which could tightly trap microparticles to the boundaries of the microstructure by tuning the applied driving frequency and voltage. Numerical simulations and experimental demonstrations illustrate that the capturing and assembly of microparticles is closely related to the size of particles, owing to the vibration-induced locally enhanced acoustic field and resultant propulsion force. This acoustic assembly strategy can open extensive opportunities for lab-on-chip systems, microfactories, and micro-manipulators, among others.

3D Full Morphometric Assessment by Holographic Imaging at Lab-on-Chip Scale for Biomedical Applications

2014 ◽  
Author(s):  
Pietro Ferraro ◽  
Pasquale Memmolo ◽  
Paolo A. Netti ◽  
Lisa Miccio ◽  
Francesco Merola
Keyword(s):  
Biomedical Applications ◽  
Lab On Chip ◽  
Holographic Imaging ◽  
On Chip ◽  
Morphometric Assessment
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