scholarly journals The Response of Gray Treefrogs to Anesthesia by Tricaine Methanesulfonate (TMS or MS-222)

ISRN Zoology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mary Paduano ◽  
Kaitlen C. Colafrancesco ◽  
Sarah A. Wong ◽  
Michael S. Caldwell ◽  
Marcos Gridi-Papp
Keyword(s):  
Body Mass ◽  
Individual Variation ◽  
Induction Time ◽  
Recovery Time ◽  
Hyla Chrysoscelis ◽  
Multiple Exposures ◽  
Lack Of Information ◽  
Gray Treefrogs ◽  
Tricaine Methanesulfonate

The design of anesthetic protocols for frogs is commonly hindered by lack of information. Results from fishes and rodents do not always apply to frogs, and the literature in anurans is concentrated on a few species. We report on the response of treefrogs (Hyla chrysoscelis and H. versicolor) to tricaine methanesulfonate. Body mass did not differ significantly between the species or between sexes. In the first exposure of a frog to TMS, variation in induction time was best explained by species (H. chrysoscelis resisted longer) and body mass (larger animals resisted longer). Multiple exposures revealed a strong effect of individual variation on induction time and a significant increase of induction time with number of previous anesthesia events within the same day. Recovery time was mostly explained by individual variation, but it increased with total time in anesthetic and decreased with induction time. It also increased with number of days since the last series of anesthesias and decreased with number of previous uses of the anesthetic bath. This is one of the first studies of anesthesia in hylids and also one of the first assessments of the factors that influence the variability of the response to anesthesia within a species.

scholarly journals Occupations and exposure events in acute and subacute irritant-induced asthma

2021 ◽  
pp. oemed-2020-107323
Author(s):  
Irmeli Lindström ◽  
Jussi Lantto ◽  
Kirsi Karvala ◽  
Satu Soini ◽  
Katriina Ylinen ◽  
...  
Keyword(s):  
Acute Onset ◽  
High Exposure ◽  
Root Cause ◽  
Multiple Exposures ◽  
Lack Of Information ◽  
Hygiene Measures ◽  
Work Tasks ◽  
Causative Agents ◽  
Strong Acids ◽  
Normal Work

BackgroundExposures leading to irritant-induced asthma (IIA) are poorly documented.MethodsWe retrospectively screened the medical records of patients with IIA diagnosed in an occupational medicine clinic during 2000–2018. We classified the cases into acute (onset after single exposure) and subacute (onset after multiple exposures) IIA. We analysed in detail, occupations, causative agents and their air levels in the workplace, exposure events and the root causes of high exposure.ResultsAltogether 69 patients were diagnosed with IIA, 30 with acute and 39 with subacute IIA. The most common occupational groups were industrial operators (n=23, 33%), metal and machinery workers (n=16, 11%) and construction workers (n=12, 8%). Among industrial operators significantly more cases had subacute IIA than acute IIA (p=0.002). Forty cases (57%) were attributable to some type of corrosive acidic or alkaline chemical. Acute IIA followed accidents at work in different types of occupation, while subacute IIA was typical among industrial operators performing their normal work tasks under poor work hygiene conditions. The most common root cause was lack of information or false guidance in acute IIA (n=11, 36%) and neglect of workplace hygiene measures in subacute IIA (n=29, 74%).ConclusionsAccidents are the main causes of acute IIA, whereas subacute IIA can develop in normal work in risk trades with poor work hygiene. Airborne strong acids or bases seem to be the most important causative agents of acute and subacute IIA. The different risk profiles of acute and subacute IIA should be considered in the prevention and identification of the cases.

scholarly journals Intraspecific Variation in Maximum Ingested Food Size and Body Mass in Varecia rubra and Propithecus coquereli

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Adam Hartstone-Rose ◽  
Jonathan M. G. Perry
Keyword(s):  
Body Size ◽  
Body Mass ◽  
Individual Variation ◽  
Size Variation ◽  
Maximum Size ◽  
Least Squares Regression ◽  
Scaling Relationship ◽  
Varecia Rubra ◽  
Food Size ◽  
The Relationship

In a recent study, we quantified the scaling of ingested food size (Vb )—the maximum size at which an animal consistently ingests food whole—and found that Vb scaled isometrically between species of captive strepsirrhines. The current study examines the relationship between Vb and body size within species with a focus on the frugivorous Varecia rubra and the folivorous Propithecus coquereli. We found no overlap in Vb between the species (all V. rubra ingested larger pieces of food relative to those eaten by P. coquereli), and least-squares regression of Vb and three different measures of body mass showed no scaling relationship within each species. We believe that this lack of relationship results from the relatively narrow intraspecific body size variation and seemingly patternless individual variation in Vb within species and take this study as further evidence that general scaling questions are best examined interspecifically rather than intraspecifically.

On the Use of Commercial Quails as Study Organisms: Lessons about Food Intake from Individual Variation in Body Mass

2012 ◽  
Vol 5 (3) ◽  
pp. 137-141 ◽  
Author(s):  
C. Duval ◽  
P. Cassey ◽  
S. Desaivre ◽  
S. J. Reynolds ◽  
K. A. Spencer
Keyword(s):  
Food Intake ◽  
Body Mass ◽  
Individual Variation

Do female frogs exploit inadvertent social information to locate breeding aggregations?

2007 ◽  
Vol 85 (9) ◽  
pp. 921-932 ◽  
Author(s):  
E.M. Swanson ◽  
S.M. Tekmen ◽  
M.A. Bee
Keyword(s):  
Social Information ◽  
Hyla Chrysoscelis ◽  
Timing Of Breeding ◽  
Sensory Cues ◽  
Bufo Americanus ◽  
Long Distance ◽  
Advertisement Calls ◽  
Inadvertent Social Information ◽  
Gray Treefrogs ◽  
Breeding Seasons

The advertisement calls of male anurans (frogs and toads) are loud and conspicuous signals, and the sound generated by breeding aggregations of males propagates over long distances. As a by-product of communication within an aggregation, the sounds of a frog chorus constitute a form of inadvertent social information that provides potential long-distance cues about the location and timing of breeding. We investigated whether female American toads ( Bufo americanus Holbrook, 1836) and Cope’s gray treefrogs ( Hyla chrysoscelis Cope, 1880) use the sounds of a chorus to locate breeding aggregations in the absence of other sensory cues. Females of both species approached speakers broadcasting recordings of a chorus made from distances of 0, 20, and 40 m, but not from distances of 80 and 160 m. Female toads also exhibited phonotaxis to a completely artificial chorus sound, but female gray treefrogs did not. We found little evidence to suggest that female American toads and Cope’s gray treefrogs differed substantially in their responses to natural chorus sounds despite potential differences in the predictability and duration of breeding seasons in these two species. Our results suggest that the inadvertent social information of a chorus could be used over short distances to locate breeding aggregations.

scholarly journals The Used of Natural Anesthetic Materials Nutmeg Seed Oil in the Transportation of Sumatran Fish Fingerlings (Puntius tetrazona)

2021 ◽  
pp. 1-10
Author(s):  
Walim Lili ◽  
Muhamad Fikri Wahyudin ◽  
Asep Agus Handaka Suryana ◽  
Kiki Haetami
Keyword(s):  
Survival Rate ◽  
Induction Time ◽  
Recovery Time ◽  
Seed Oil ◽  
Fish Mortality ◽  
Metabolic Disturbances ◽  
Two Factors ◽  
Test Fish ◽  
Four Levels ◽  
And Control

The low survival rate in Sumatran fish fingerlings transportation is a problem related to metabolic disturbances that cause death. The addition of nutmeg seed oil in the transportation medium is expected to reduce the rate of respiration and metabolism, so that fish mortality can be minimized. The purpose of this study was to determine the effective concentration of nutmeg seed oil to maintain the highest survival rate of Sumatran fish fry transported during treatment and to analyze its effect on induction time and recovery time. This research was carried out from January to March 2021 in the Ciparanje wet laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research was conducted experimentally using a factorial randomized group design (FRGD) consisting of two factors, namely the concentration of four levels (0.03, 0.06, 0.09 ml/L and control) and duration of three levels (3, 5, and 7 hours) which was repeated three times. This research uses a closed transportation system. Parameters observed were induction time and conscious recovery time of test fish, post-transportation and post-maintenance survival for 7 days and water quality consisting of temperature, DO, pH, and ammonia. The results showed that the effective nutmeg seed oil for the anesthesia of Sumatran fish fry transported was 0.03 ml/L with a transportation duration of 3 hours because it had an induction time of 08:07, recovery time of 02:41 and postoperative survival rate. transportation by 98.33% and post-maintenance for 7 days by 91.49%. Temperature parameters are 22.7oC, DO is ±10.3 mg/L, pH is ± 6.83 and ammonia is ±0.0010 mg/L.

scholarly journals The Use of Natural Anesthetic Ingredients for Clove Oil in Transportation of Juvenile Swordtail (Xiphophorus helleri)

2021 ◽  
pp. 1-8
Author(s):  
Kelvin Manik ◽  
Walim Lili ◽  
. Rosidah ◽  
Roffi Grandiosa
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Effective Treatment ◽  
Induction Time ◽  
Recovery Time ◽  
Block Design ◽  
Effective Concentration ◽  
Clove Oil ◽  
Xiphophorus Helleri ◽  
Randomized Block Design

This research aims to determine the most effective concentration of clove oil and the best period in the transportation of swordtail (Xiphophorius helleri) with the highest survival rate. This research used the factorial randomized block design (FRBD) which consisting of four treatments of concentration (10 x 10-3 mL, 13 x 10-3 mL, 16 x 10-3 mL), three treatments of duration (3 hours, 5 hours, 7 hours), and repeated three times. The measured parameters are induction time, conscious recovery time, and survival rate after transportation. The results showed that the treatment of 10 x 10-3 mL with the duration of 5 hours was an effective treatment with an average induction time of 17 minutes 56 seconds, a conscious recovery time of 7 minutes 37 seconds, the survival rate of 100% at post-transportation and after 7 days of rearing is 83%. Water quality after transportation are temperature (24.6 ℃), DO (14.72 mg / L), pH (6.42) and ammonia (0.0043 mg/L).

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