scholarly journals The Used of Natural Anesthetic Materials Nutmeg Seed Oil in the Transportation of Sumatran Fish Fingerlings (Puntius tetrazona)

Author(s):  
Walim Lili ◽  
Muhamad Fikri Wahyudin ◽  
Asep Agus Handaka Suryana ◽  
Kiki Haetami

The low survival rate in Sumatran fish fingerlings transportation is a problem related to metabolic disturbances that cause death. The addition of nutmeg seed oil in the transportation medium is expected to reduce the rate of respiration and metabolism, so that fish mortality can be minimized. The purpose of this study was to determine the effective concentration of nutmeg seed oil to maintain the highest survival rate of Sumatran fish fry transported during treatment and to analyze its effect on induction time and recovery time. This research was carried out from January to March 2021 in the Ciparanje wet laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research was conducted experimentally using a factorial randomized group design (FRGD) consisting of two factors, namely the concentration of four levels (0.03, 0.06, 0.09 ml/L and control) and duration of three levels (3, 5, and 7 hours) which was repeated three times. This research uses a closed transportation system. Parameters observed were induction time and conscious recovery time of test fish, post-transportation and post-maintenance survival for 7 days and water quality consisting of temperature, DO, pH, and ammonia. The results showed that the effective nutmeg seed oil for the anesthesia of Sumatran fish fry transported was 0.03 ml/L with a transportation duration of 3 hours because it had an induction time of 08:07, recovery time of 02:41 and postoperative survival rate. transportation by 98.33% and post-maintenance for 7 days by 91.49%. Temperature parameters are 22.7oC, DO is ±10.3 mg/L, pH is ± 6.83 and ammonia is ±0.0010 mg/L.

Author(s):  
Kelvin Manik ◽  
Walim Lili ◽  
. Rosidah ◽  
Roffi Grandiosa

This research aims to determine the most effective concentration of clove oil and the best period in the transportation of swordtail (Xiphophorius helleri) with the highest survival rate. This research used the factorial randomized block design (FRBD) which consisting of four treatments of concentration (10 x 10-3 mL, 13 x 10-3 mL, 16 x 10-3 mL), three treatments of duration (3 hours, 5 hours, 7 hours), and repeated three times. The measured parameters are induction time, conscious recovery time, and survival rate after transportation. The results showed that the treatment of 10 x 10-3 mL with the duration of 5 hours was an effective treatment with an average induction time of 17 minutes 56 seconds, a conscious recovery time of 7 minutes 37 seconds, the survival rate of 100% at post-transportation and after 7 days of rearing is 83%. Water quality after transportation are temperature (24.6 ℃), DO (14.72 mg / L), pH (6.42) and ammonia (0.0043 mg/L).


Author(s):  
Ainun Ayu Utami Amris ◽  
Sri Wahyuni Rahim ◽  
Khusnul Yaqin

The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time  and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).


2021 ◽  
pp. 1059-1066
Author(s):  
Tatyana Vladimirovna Zubova ◽  
Vladimir Alexandrovich Pleshkov ◽  
Oksana Vladimirovna Smolovskaya ◽  
Alexander Nikolaevich Mironov ◽  
Larisa Nikolaevna Korobeynikova

Background and Aim: It is known that metabolic disturbances in the animal body negatively affect parturition, uterine involution, and, ultimately, fertility, especially in cows, during the first insemination. Although the method of diet optimization with the use of a software package results in positive outcomes, adjustment is required for certain groups of animals (e.g., cows), in accordance with the period of the year. Every year, in the spring and autumn, blood is taken from animals to detect metabolic disorders, and then either the diet is balanced or, if the cows lack vitamins and minerals, they are administered to cows parenterally or with food. The aim of this study was to assess the efficiency of using carotene-containing preparation in the prevention of postpartum complications in cows. Materials and Methods: Before the start of the experiment, blood was collected from the animals of the experimental and control groups, with ten animals in each group, and its serum was examined for the presence of carotene. Low carotene content was found in the serum of all animals (from 0.2 to 0.25 mg%) with the norm within the range of 0.40-0.62 mg%. The cows of the experimental group were injected subcutaneously with the carotene-containing preparation 30, 20, and 10 days before the expected calving date at a dose of 10 mL per head. The carotene-containing preparation was a solution of crystalline β-carotene substance in deodorized sunflower oil. Moreover, the share of β-carotene was at least 0.18%. The drug was administered intramuscularly into the rump. Results: In the postpartum period, the retention of the placenta was observed in two animals of the control group. The uterine involution in the cows of the control group was 16.0 (p<0.05) days longer than that in the cows of the experimental group. The duration of placenta separation in the cows of the control group was on average 3.21 h longer (p<0.01) than that in the cows of the experimental group. The period from calving to the introduction of the embryo was 63.17±1.56 days in the control group and 48.3±0.83 days in the experimental group. The survival rate of embryos in the cows of the experimental group was 60%, and the period from the calving date to the introduction of the embryo averaged 48 days, which were 14.9 (p<0.05) days less than that in the cows of the control group. Conclusion: When the carotene-containing preparation was administered in a dose of 10 mL subcutaneously to cows 30, 20, and 10 days before the calving date, the blood carotene content increased, and the duration of the last stage and uterine involution decreased. The period from the calving date to the introduction of the embryo was reduced to 48.3±0.83 days, and the survival rate of embryos was 60%.


2018 ◽  
Vol 6 (1) ◽  
pp. 117
Author(s):  
Ramlesius Mangaro ◽  
Hengky J. Sinjal ◽  
Revol D. Monijung

This research activity was conducted at Balai Pembenihan, Pengendalian Hama dan Penyakit Tateli from August -September 2017. The treatment in this study was masculinization by using Immersion Method (P), Oral (O) and Control or without hormone addition (K). In the method of immersion with a dose of 10 mg / L water / 100 larvae in soak for 6 hours. Oral Method with a dose of 15 mg / kg of feed hormone, administration for 14 days. Control is maintenance without treatment. The result of male sex test identification at the end of the experiment was obtained by the percentage of males after the highest treatment was Oral (O) treatment of 92.57%, the immersion treatment (P) of 89.53%, and the last was Control (K) , 73%. The survival rate (SR) of the test fish during the 50 days of trial maintenance was highest in Oral test fish, 61.67% followed by Immersion 53% and 38.67% Control. Keywords : Fish,  Immersionn, Masculinization, Oral, Survival, TilapiaAbstrakPenelitian ini dilakukan di Balai Pembenihan, Pengendalian hama dan Penyakit Tateli dari bulan Agustus – September 2017.  Perlakuan dalam penelitian ini adalah maskulinisasi dengan menggunakan metode Peredaman (P), Oral (O) dan Kontrol atau tanpa penambahan hormon (K). Pada metode Perendaman dengan dosis 10 mg/L air/100 ekor larva di rendam selama 6 jam. Metode Oral dengan dosis hormon 15 mg/Kg pakan, pemberian selama 14 hari. Kontrol adalah pemeliharaan tanpa perlakuan. Hasil identifikasi kelamin jantan ikan uji pada akhir percobaan didapat persentase jantan setelah perlakuan tertinggi adalah perlakuan Oral (O) sebesar 92,57 %, perlakuan Perendaman (P) sebesar 89,53 %, dan yang terakhir adalah Kontrol (K) dengan hasil sebesar 52,73 %. Persentase kelangsungan hidup (SR) ikan uji selama pemeliharaan 50 hari percobaan adalah yang tertinggi terdapat pada ikan uji Oral  yaitu sebesar 61,67%  diikuti dengan Perendaman sebesar 53 % dan Kontrol sebesar 38,67  %.Kata kunci : Ikan, Maskulinisasi, Perendaman, Oral, Kelangsungan Hidup, Nila


2015 ◽  
Vol 4 (2) ◽  
pp. 50-55
Author(s):  
Sandra J Nendissa ◽  
Rachel Breemer ◽  
Nikholaus Melamas

This objectives of this research were both to study and determine the best level of concentration of yeast Saccharomyces cereviseae and period of fermentation on the quality of tomi-tomi vinegar (Flacourtia inermis). A completely randomized experimental design with two factors of treatment was applied in this research. The first factor was concentration of yeast S. cereviseae having four levels of tretament, i.e.: without the addition of yeast 0.5, 1 and 1.5 g yeast. The second factor was period fermentation with 1, 2, 3, 4, and 5 weeks. The result indicated that the concentration of yeast S. cereviseae 1.5 g and period fermentation 5 week produced a good tomi-tomi vinegar with total acids 51.22%, total dissolved solids 8.35, total sugar 8.07% and pH 5.40.


2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Muhammad Atilah Setiawan ◽  
Muarif Muarif ◽  
Fia Sri Mumpuni

The aim of this study was to determine the growth and survival rate of Ctenopoma fish seeds. This study used a completely randomized design with 3 treatments and 3 replications. The treatment in this study was a different feeding rate using natural feed of Tubifex sp  namely A (8%), B (10%), and C (12%) ). The test fish used was Ctenopoma fish seeds with an initial length of 2.40 ± 0.10 cm and an initial weight of 0.32 ± 0.05 g. Fish seeds are cultivated  for 28 days with feeding frequency twice a day. The results of research showed that the use of different feeding rate had an significantly different (P<0.05) on the specific growth  rate and absolute length growth with the best results in the treatment C. The survival rate was not significantly different between treatments (P>0.05). Key Words: Ctenopoma, feeding rate, growth, survival rate 


2021 ◽  
Vol 28 ◽  
pp. 107327482199743
Author(s):  
Ke Chen ◽  
Xiao Wang ◽  
Liu Yang ◽  
Zheling Chen

Background: Treatment options for advanced gastric esophageal cancer are quite limited. Chemotherapy is unavoidable at certain stages, and research on targeted therapies has mostly failed. The advent of immunotherapy has brought hope for the treatment of advanced gastric esophageal cancer. The aim of the study was to analyze the safety of anti-PD-1/PD-L1 immunotherapy and the long-term survival of patients who were diagnosed as gastric esophageal cancer and received anti-PD-1/PD-L1 immunotherapy. Method: Studies on anti-PD-1/PD-L1 immunotherapy of advanced gastric esophageal cancer published before February 1, 2020 were searched online. The survival (e.g. 6-month overall survival, 12-month overall survival (OS), progression-free survival (PFS), objective response rates (ORR)) and adverse effects of immunotherapy were compared to that of control therapy (physician’s choice of therapy). Results: After screening 185 studies, 4 comparative cohort studies which reported the long-term survival of patients receiving immunotherapy were included. Compared to control group, the 12-month survival (OR = 1.67, 95% CI: 1.31 to 2.12, P < 0.0001) and 18-month survival (OR = 1.98, 95% CI: 1.39 to 2.81, P = 0.0001) were significantly longer in immunotherapy group. The 3-month survival rate (OR = 1.05, 95% CI: 0.36 to 3.06, P = 0.92) and 18-month survival rate (OR = 1.44, 95% CI: 0.98 to 2.12, P = 0.07) were not significantly different between immunotherapy group and control group. The ORR were not significantly different between immunotherapy group and control group (OR = 1.54, 95% CI: 0.65 to 3.66, P = 0.01). Meta-analysis pointed out that in the PD-L1 CPS ≥10 sub group population, the immunotherapy could obviously benefit the patients in tumor response rates (OR = 3.80, 95% CI: 1.89 to 7.61, P = 0.0002). Conclusion: For the treatment of advanced gastric esophageal cancer, the therapeutic efficacy of anti-PD-1/PD-L1 immunotherapy was superior to that of chemotherapy or palliative care.


2021 ◽  
Vol 883 (1) ◽  
pp. 012052
Author(s):  
N Herawati ◽  
A R Aisah ◽  
I Mardian ◽  
B N Hidayah ◽  
B T R Erawati

Abstract Plant growth is influenced by seed quality. Seeds need to be treated to prevent pest and disease disorders or to increase seed germination. Soybean planting was carried out in this study by the treatment of varieties and seed pre-treatment application before planting. The study aimed to measure the growth and yield of soybeans by varieties and seed treatment before planting, carried out in the Village of Nggembe, District of Bolo, Bima Regency. The study used factorial randomized block design with two factors. The first factor was soybean varieties consisting of two levels, namely Devon and Dena varieties, and the second factor was the application of pre-planting seed treatments consisting of four levels namely Cruiser, Marshall, Agrisoy, and without seed treatment, each treatment was repeated three times. Observations were made on the performance of agronomic crops and soybean yields. The results showed that the treatment of soybean varieties and the application of seed treatment had a significant influence on plant height and soybean productivity, and both treatments had interactions on parameters of plant height, number of filled pods, weight of 100 seeds, and soybean productivity with the best results successively produced by a combination of Devon-Control, Devon-Marshall, Devon-Agrisoy, and Dena-Cruiser.


2018 ◽  
Vol 13 (2) ◽  
pp. 95 ◽  
Author(s):  
Nurbaya Nurbaya ◽  
Muharijadi Atmomarsono

To counter disease problems caused by vibriosis in shrimp hatchery, this recent study used three different probiotics to be tested on tiger shrimp (Penaeus monodon) postlarvae. The study arranged four treatments as follows: A: a combination of three liquid-form probiotics Brevibacillus laterosporus BT951, Bacillus subtilis BM12, and B. licheniformis BM58; B: a combination of three powder-form probiotics Brevibacillus laterosporus BT951, Bacillus subtilis BM12, and B. licheniformis BM58; C: a commercial powder probiotic containing Bacillus subtilis; and D: control (without probiotic), each treatment with three replications. This study was set up in a completely randomized design experiment using twelve fiberglass tanks filled with 750 L sterile sea water and stocked with 30,000 nauplii in the Awarange shrimp hatchery of the Research Institute for Brackishwater Aquaculture and Fisheries Extension Installation in Barru. Variables observed in this study were the survival rate of the shrimp postlarvae at the end of the experiment, total vibrio count (TBV) and total plate count of common bacteria (TPC) in the culture water. The results showed that the survival rate of tiger shrimp applied either in liquid (A: 61.5±4.7%) or powder form (B: 48.6±6.8%), and control (without probiotic) (D: 51.2±4.4%) were not significantly different (P>0.05). However, survival rates in these three treatments differed (P<0.05) with that of the commercial probiotic (C: 21.7±9.9%). TBV/TPC ratio in the tank waters treated with the commercial probiotic (2.26-37.52%) was much higher than that of the liquid form probiotic (0.86-1.98%), powder form probiotic (1.25-8.37%), and control (1.93-2.84%). Ammonia-nitrogen in treatment C (1.462-2.989 mg/L) was relatively higher than that of in treatment A (1.595-2.435 mg/L), treatment B (1.644-2.115 mg/L), and treatment D (1.051-1.858 mg/L).


2019 ◽  
pp. 117-120
Author(s):  
Stephanie Imelda Pella ◽  
Hendro FJ L

This research presents an automation process of controlling room temperature based on the number of people detected in a room. The system consists of a single board raspberry pi computer, esp8266 micro controller, pi camera, and an infrared module. This research is divided into two parts, namely object detection using Raspbery Pi and Tensorflow and Open CV libraries and controlling air cooling system (ACS) using esp8266 and infrared modules by transmitting hexadecimal AC control codes. The ACS temperature is divided into four levels with a minimum value at 18o C and a maximum at 24o C. System testings were carried out in an empty room and a room with a capacity of 50 people that is fully occupied. The results show that the system is able to detect the number of people in the room and control the ACS, but under certain conditions some objects are not detected because the position and camera tilt is not optimal.


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