scholarly journals The Cytotoxic Effect of Magainin II on the MDA-MB-231 and M14K Tumour Cell Lines

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Radu Anghel ◽  
Daniela Jitaru ◽  
Laurenţiu Bădescu ◽  
Magda Bădescu ◽  
Manuela Ciocoiu
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Chemotherapeutic Agents ◽  
Breast Adenocarcinoma ◽  
Public Health Issue ◽  
Tumour Cell Line ◽  
Global Public Health ◽  
Tumour Cell Lines ◽  
Magainin Ii

Many studies have highlighted the tumoricidal properties of some natural peptides known to have antimicrobial virtues. Also, the increasingly higher resistance to conventional antibiotics has become a global public health issue, and the need for new antibiotics has stimulated interest in finding and synthesizing new antimicrobial peptides, which may also be used as chemotherapeutic agents. Relying on the literature, the purpose of ourin vitroresearch was to assess the tumoricidal potential of magainin II on a series of tumour cell lines, namely, MDA-MB-231 (breast adenocarcinoma) and M14K (human mesothelioma). The experimental results of our study revealed that the cytotoxic effects of magainin II depend on its concentration. Its efficiency is significant at 120 μM concentrations, and, although it is much lower, it persists even at 60 μM concentrations. The effects were insignificant at 30 μM concentrations. In our experimental research, the tumoricidal effect of magainin II was not significantly dependent on the type of tumour cell line used.

scholarly journals Masked Phenolic-Selenium Conjugates: Potent and Selective Antiproliferative Agents Overcoming P-gp Resistance

2020 ◽  
Vol 13 (11) ◽  
pp. 358
Author(s):  
Paloma Begines ◽  
Lucía Sevilla-Horrillo ◽  
Adrián Puerta ◽  
Rebecca Puckett ◽  
Samuel Bayort ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Efflux Pump ◽  
Chemotherapeutic Agents ◽  
Cytotoxic Agents ◽  
Therapeutic Approaches ◽  
Vast Number ◽  
Antiproliferative Agents ◽  
Multifactorial Diseases ◽  
Tumour Cell Lines

Cancer accounts for one of the most complex diseases nowadays due to its multifactorial nature. Despite the vast number of cytotoxic agents developed so far, good therapeutic approaches are not always reached. In recent years, multitarget drugs are gaining great attention against multifactorial diseases in contraposition to polypharmacy. Herein we have accomplished the conjugation of phenolic derivatives with an ample number of organochalcogen motifs with the aim of developing novel antiproliferative agents. Their antioxidant, and antiproliferative properties (against six tumour and one non-tumour cell lines) were analysed. Moreover, in order to predict P-gp-mediated chemoresistance, the P-glycoprotein assay was also conducted in order to determine whether compounds prepared herein could behave as substrates of that glycoprotein. Selenium derivatives were found to be significantly stronger antiproliferative agents than their sulfur isosters. Moreover, the length and the nature of the tether, together with the nature of the organoselenium scaffold were also found to be crucial features in the observed bioactivities. The lead compound, bearing a methylenedioxyphenyl moiety, and a diselenide functionality, showed a good activity (GI50 = 0.88‒2.0 µM) and selectivity towards tumour cell lines (selectivity index: 14‒32); moreover, compounds considered herein were not substrates for the P-gp efflux pump, thus avoiding the development of chemoresistance coming from such mechanism, commonly found for widely-used chemotherapeutic agents.

Immunophenotype of Mammary Tumour Cell Lines for In-Vitro Analysis Using Three Different Techniques

2020 ◽  
Vol 174 ◽  
pp. 184
Author(s):  
F. Torrigiani ◽  
A. Sammarco ◽  
M.E. Gelain ◽  
F. Bonsembiante ◽  
R. Zanetti ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Lines ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Novel O-methyl goniofufurone and 7-epi-goniofufurone derivatives: synthesis, in vitro cytotoxicity and SAR analysis

MedChemComm ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 2017-2027
Author(s):  
Jovana Francuz ◽  
Mirjana Popsavin ◽  
Sanja Djokić ◽  
Vesna Kojić ◽  
Tatjana Srdić-Rajić ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Methoxy Group ◽  
Antitumour Activity ◽  
In Vitro Cytotoxicity ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Sar Analysis ◽  
Human Tumour Cell Lines

Novel goniofufurone (1) and 7-epi-goniofufurone (2) derivatives bearing a methoxy group at the C-5 and/or C-7 positions were prepared and their in vitro antitumour activity against some human tumour cell lines was evaluated.

scholarly journals Synthesis, Characterization and High In Vitro Antitumour Activity of Novel Triphenyltin Carboxylates

1994 ◽  
Vol 1 (4) ◽  
pp. 305-309 ◽  
Author(s):  
Marcel Gielen ◽  
Abdelaziz El Khloufi ◽  
Monique Biesemans ◽  
Abdeslam Bouhdid ◽  
Dick de Vos ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Cell Lines ◽  
Tumour Cell ◽  
Antitumour Activity ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Human Tumour Cell Lines ◽  
Mcf 7

The synthesis and spectral characterization of six novel triphenyltin compounds are described. The in vitro antitumour activity of three of these compounds against two human tumour cell lines, MCF-7, a mammary tumour, and WiDr, a colon carcinoma, was determined. All three compounds are more active than cis-platin, etoposide and doxorubicin against both tumour cell lines. They are as active as mitomycin C against WiDr, but less active against MCF-7.

In-vitro anti-proliferative effects of some anti-tumour drugs on feline mammary tumour cell lines

1999 ◽  
Vol 66 (3) ◽  
pp. 169-174 ◽  
Author(s):  
J.S MULEYA ◽  
M NAKAICHI ◽  
Y TAURA ◽  
R YAMAGUCHI ◽  
S NAKAMA
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Lines

scholarly journals Di-n-Butyl-, Tri-n-Butyl- and Triphenyltin dl-Terebates: Synthesis, Characterization and In Vitro Antitumour Activity

1997 ◽  
Vol 4 (4) ◽  
pp. 193-197 ◽  
Author(s):  
Marcel Gielen ◽  
Huairang Ma ◽  
Abdeslam Bouhdid ◽  
Hassan Dalil ◽  
Monique Biesemans ◽  
...  
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Antitumour Activity ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Human Tumour Cell Lines

Di-n-butyltin, tri-n-butyltin and triphenyltin terebates were screened against several human tumour cell lines and found comparably or more active than carboplatin, cis-platin, 5-fluorouracil, methotrexate and doxorubicin, some reference compounds used clinically.

l-Asparaginyl-Trna Synthetase and l-Asparagine Synthetase Activities of l-Asparaginase-Sensitive and -Resistant Forms of the Mouse Gardner Lymphoma 6C3HED

1979 ◽  
Vol 56 (6) ◽  
pp. 539-545 ◽  
Author(s):  
M. R. Davies ◽  
Lucy P. Lambert ◽  
R. D. Marshall
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Trna Synthetase ◽  
Asparagine Synthetase ◽  
Ascites Fluid ◽  
Synthetase Activity ◽  
Early Passage ◽  
Tumour Cell Lines ◽  
Cells Cultured

1. The mouse Gardner lymphoma 6C3HED was grown in ascites fluid in a form sensitive to the action of l-asparaginase (line 1), in another form which was resistant to l-asparaginase (line 2) and in a third form with partial sensitivity to l-asparaginase (line 3). 2. The l-asparaginyl-tRNA synthetase activities of extracts of the tumour cells, cultured both in the mouse and in vitro, were determined. Two of the lines, 1 and 3, in early passage numbers, showed a derepression mechanism involving l-asparagine. Mutation occurred with these lines resulting in the l-asparaginyl-tRNA synthetase activity of all the tumour cell lines being the same. 3. Cells of line 1 had low l-asparagine synthetase activity, which was unchanged by altering the supply of l-asparagine in vitro. Cells of lines 2 and 3 exhibited l-asparagine synthetase activities, which changed with the supply of l-asparagine. 4. It is not certain that l-asparagine synthetase activity of l-asparaginase-sensitive cells is controlled by l-asparaginyl-tRNA acting as a corepressor.

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