scholarly journals Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Letícia A. de C. Lara ◽  
João B. dos Santos ◽  
Juliana S. Veloso ◽  
Marcio Balestre ◽  
Filipe C. Alves ◽  
...  

The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F2 population of common bean evaluated by the straw test method. The DNA from 186 F2 plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F2:4 progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F2:4 progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance—13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection.

2011 ◽  
Vol 11 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Flávia Fernandes Carneiro ◽  
João Bosco dos Santos ◽  
Paulo Roberto Carvalho Gonçalves ◽  
Rafaela Priscila Antonio ◽  
Thaís Paula de Souza

The objective of the research was to investigate the nature and magnitude of the genetic factors involved in the resistance of the common bean to white mold. The lines G122 (resistant) and M20 (susceptible) were crossed to yield F1 and F2 generations and F2:3 progenies. The experiment was set up using the random block design with two replications, each of which was evaluated twice with fungal inoculations being performed on 28 and 38 day-old plants using the straw test method. Six to eight days after inoculation evaluations were conducted on individual plants and at the level of means of progenies using a diagrammatic scale ranging from 1 to 9. The additive-dominance model adopted was efficient, and the genetic control of resistance was predominantly due additive effects. Estimates of broad-sense heritability indicated that selection would be more efficient when based on the means of progenies and when successive inoculations are employed.


2007 ◽  
Vol 21 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Marcio Ender ◽  
Karolyn Terpstra ◽  
James D. Kelly

2009 ◽  
Vol 3 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Shree P. Singh ◽  
Henry Terán ◽  
Howard F. Schwartz ◽  
Kristen Otto ◽  
Margarita Lema
Keyword(s):  

Author(s):  
George Muhamba ◽  
Luseko Amos ◽  
Deogracious Protas ◽  
Paul Mbogo ◽  
Susan Nchimbi-Msoll

Crop Science ◽  
2012 ◽  
Vol 52 (4) ◽  
pp. 1511-1521 ◽  
Author(s):  
Robert W. Duncan ◽  
Robert L. Gilbertson ◽  
Shree P. Singh

2009 ◽  
Vol 89 (4) ◽  
pp. 755-762 ◽  
Author(s):  
H Terán ◽  
S P Singh

White mold (WM) caused by Sclerotinia sclerotiorum (Lib.) de Bary is the most devastating disease of common bean (dry and snap or garden bean) (Phaseolus vulgaris L.) in North America. The use of a reliable screening method (SM) in common bean is crucial to improve physiological resistance to WM. The objective of this study was to compare the efficacy of three SM to identify physiological resistance in dry bean genotypes with different evolutionary origins and levels of resistance. Screening methods tested were: (i) the modified straw test or cut–stem (CSM); (ii) infected bean flower (IFL); and (iii) infected oat seed (IOS). A 195, ICA Bunsi, Othello, and VCW 54 dry bean were tested with the three SM. The experimental design was a split plot in randomized complete blocks with three replications in 2007 and 2008. Two independent inoculations 1 wk apart for each SM were made. The WM reaction was scored at 16, 23, and 33 d post-inoculation (DPI) using a 1 to 9 scale. There were highly significant differences between SM and its interaction with years. The CSM and IFL were the most consistent and highly correlated (r > 0.70, P < 0.01). Interspecific breeding line VCW 54 consistently had the highest WM resistance across years, SM, and evaluation dates, followed by A 195. White mold scores increased with delayed evaluations. Thus, CSM or IFL with disease assessed 33 DPI should be used for identifying common bean genotypes with high levels of physiological resistance to WM.Key words: Common bean, growth habit, race Mesoamerica, race Nueva Granada, Phaseolus vulgaris, Sclerotinia sclerotiorum


2015 ◽  
Vol 14 (1) ◽  
pp. 1124-1135 ◽  
Author(s):  
L.A.C. Lara ◽  
J.B. Santos ◽  
M. Balestre ◽  
I.A. Lima ◽  
A.K.A. Pamplona ◽  
...  

2019 ◽  
Vol 4 (1) ◽  
pp. 41-55 ◽  
Author(s):  
Jan Hummel ◽  
Dietmar Göhlich ◽  
Roland Schmehl

Abstract. We have developed a tow test setup for the reproducible measurement of the dynamic properties of different types of tethered membrane wings. The test procedure is based on repeatable automated maneuvers with the entire kite system under realistic conditions. By measuring line forces and line angles, we determine the aerodynamic coefficients and lift-to-drag ratio as functions of the length ratio between power and steering lines. This nondimensional parameter characterizes the angle of attack of the wing and is varied automatically by the control unit on the towed test bench. During each towing run, several test cycles are executed such that mean values can be determined and errors can be minimized. We can conclude from this study that an objective measurement of specific dynamic properties of highly flexible membrane wings is feasible. The presented tow test method is suitable for quantitatively assessing and comparing different wing designs. The method represents an essential milestone for the development and characterization of tethered membrane wings as well as for the validation and improvement of simulation models. On the basis of this work, more complex maneuvers and a full degree of automation can be implemented in subsequent work. It can also be used for aerodynamic parameter identification.


2018 ◽  
Vol 44 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Lenio U. Ferreira ◽  
Victor A. Ribeiro ◽  
Patrícia G. S. Melo ◽  
Murillo Lobo Junior ◽  
Joaquim Geraldo C. Costa ◽  
...  

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