Matrix Metalloproteinase-9/Neutrophil Gelatinase-Associated Lipocalin Complex Activity in Human Glioma Samples Predicts Tumor Presence and Clinical Prognosis

Matrix metalloproteinase-9/neutrophil gelatinase-associated lipocalin (MMP-9/NGAL) complex activity is elevated in brain tumors and may serve as a molecular marker for brain tumors. However, the relationship between MMP-9/NGAL activity in brain tumors and patient prognosis and treatment response remains unclear. Here, we compared the clinical characteristics of glioma patients with the MMP-9/NGAL activity measured in their respective tumor and urine samples. Using gelatin zymography assays, we found that MMP-9/NGAL activity was significantly increased in tumor tissues (TT) and preoperative urine samples (Preop-1d urine). Activity was reduced by seven days after surgery (Postop-1w urine) and elevated again in cases of tumor recurrence. The MMP-9/NGAL status correlated well with MRI-based tumor assessments. These findings suggest that MMP-9/NGAL activity could be a novel marker to detect gliomas and predict the clinical outcome of patients.

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Evaluation of neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase-9 (MMP-9) and their complex MMP-9/NGAL in sera and urine of patients with kidney tumors

Oncology Letters ◽

10.3892/ol.2013.1252 ◽

2013 ◽

Vol 5 (5) ◽

pp. 1677-1681 ◽

Cited By ~ 28

Author(s):

ANGELINA DI CARLO

Keyword(s):

Matrix Metalloproteinase ◽

Matrix Metalloproteinase 9 ◽

Kidney Tumors ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Neutrophil Gelatinase ◽

Metalloproteinase 9

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Upregulation of the Extracellular Matrix Remodeling Genes, Biglycan, Neutrophil Gelatinase-Associated Lipocalin and Matrix Metalloproteinase-9 in Familial Amyloid Polyneuropathy

Amyloid and Amyloidosis ◽

10.1201/9781420037494.ch109 ◽

2004 ◽

pp. 320-322

Author(s):

M Saraiva ◽

A Guimarães ◽

M Sousa ◽

J Amaral

Keyword(s):

Extracellular Matrix ◽

Matrix Metalloproteinase ◽

Familial Amyloid Polyneuropathy ◽

Matrix Metalloproteinase 9 ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Amyloid Polyneuropathy ◽

Neutrophil Gelatinase ◽

Metalloproteinase 9

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Up‐regulation of the extracellular matrix remodeling genes, biglycan, neutrophil gelatinase‐associated lipocalin and matrix metalloproteinase‐9 in familial amyloid polyneuropathy

The FASEB Journal ◽

10.1096/fj.04-2022fje ◽

2004 ◽

Vol 19 (1) ◽

pp. 124-126 ◽

Cited By ~ 50

Author(s):

Mónica Mendes Sousa ◽

José Barbas Amaral ◽

António Guimarães ◽

Maria João Saraiva

Keyword(s):

Extracellular Matrix ◽

Matrix Metalloproteinase ◽

Familial Amyloid Polyneuropathy ◽

Matrix Metalloproteinase 9 ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Amyloid Polyneuropathy ◽

Neutrophil Gelatinase ◽

Metalloproteinase 9

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Serum concentrations of atherogenic proteins neutrophil gelatinase-associated lipocalin and its complex with matrix metalloproteinase-9 are significantly lower in women with polycystic ovary syndrome: hint of a protective mechanism?

Acta Endocrinologica ◽

10.1530/eje-07-0822 ◽

2008 ◽

Vol 158 (4) ◽

pp. 525-531 ◽

Cited By ~ 24

Author(s):

Evanthia Diamanti-Kandarakis ◽

Sarantis Livadas ◽

Stylianos A Kandarakis ◽

Alexandra Margeli ◽

Ioannis Papassotiriou

Keyword(s):

Polycystic Ovary Syndrome ◽

Matrix Metalloproteinase ◽

Polycystic Ovary ◽

Matrix Metalloproteinase 9 ◽

Protective Mechanism ◽

Ovary Syndrome ◽

Neutrophil Gelatinase Associated Lipocalin ◽

Plaque Erosion ◽

Neutrophil Gelatinase ◽

Metalloproteinase 9

BackgroundNeutrophil gelatinase-associated lipocalin (NGAL) and matrix metalloproteinase-9 (MMP-9) have been considered as important mediators of vascular remodeling and plaque instability. The formation of a complex with NGAL and MMP-9 is crucial for atherotic plaque erosion and thrombus formation. In women with polycystic ovary syndrome (PCOS), the incidence of cardiovascular clinical events is not increased, despite the fact that they display a wide spectrum of risk factors. Since the instability of atherosclerotic plaque is a key factor in the clinical manifestations of cardiovascular disease, molecules challenging the plaque stability should be investigated.AimTo determine serum levels of NGAL and MMP-9/NGAL complex in women with PCOS.Subjects and methodsPCOS subjects (40) were compared with those (40) matched for age and body mass index (BMI) controls. In each subject, fasting levels of glucose, insulin, gonadotropins, estradiol, androgens, C-reactive protein, NGAL, and MMP-9/NGAL were determined.ResultsNGAL and MMP-9/NGAL complex levels were significantly lower in the PCOS group compared with controls (30.4±24.3 vs 70.7±37.9 μg/l, P<0.0001) and (31.5±26.6 vs 115.1±66.9 μg/l, P<0.0001) respectively. When patients and controls were stratified according to BMI, it was shown that NGAL and MMP-9/NGAL levels were significantly lower in lean (P<0.0002 and P<0.0001 respectively) and overweight (P<0.0004 and P<0.002 respectively) PCOS subjects compared with controls.ConclusionsThese findings indicate that NGAL and MMP-9/NGAL complex, two molecules that activate atherotic plaque erosion, is in lower concentrations in PCOS subjects. The role of NGAL and MMP-9/NGAL complex needs to be further investigated, since suppression of these atheromatous molecules might have a protective role in women with PCOS.

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