scholarly journals Genetic Diversity ofMycobacterium tuberculosisComplex Isolated from Tuberculosis Patients in Bahir Dar City and Its Surroundings, Northwest Ethiopia

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Anwar Nuru ◽  
Gezahegne Mamo ◽  
Adane Worku ◽  
Aschalew Admasu ◽  
Girmay Medhin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Northwest Ethiopia ◽  
Discriminatory Power ◽  
Geographical Region ◽  
Zoonotic Potential ◽  
Bahir Dar ◽  
East African ◽  
Tuberculosis Patients

The knowledge of the diversity of strains ofMycobacterium tuberculosiscomplex (MTBC) species in a specific geographical region can contribute to the control of tuberculosis (TB). This study was conducted to identify the MTBC isolates to the species and spoligotype international type (SIT) level by spoligotyping. A total of 168 MTBC isolates were recovered from TB patients, spoligotyped, and their patterns were compared with those of the strains registered in the SITVIT2 database. Of 168 isolates spoligotyped, 89 patterns were identified. Ninety-eight isolates were clustered into 19 strain groups with clustering percentage of 58.3%. Forty-four strains matched the preexisting SITs in the SITVIT2 database. The dominant strains were SIT289, SIT134, and SIT3411, comprising 16.7% (28/168), 7.14% (12/168), and 4.76% (8/168) of the isolates, respectively. Euro-American (51.2%), East-African-Indian (34.5%), andM. africanum(9.52%) were the major lineages identified. Two strains ofM. boviswere isolated from TB lymphadenitis cases. The high percentage of clustered strains ofM. tuberculosiscould suggest that a small number of lineages ofM. tuberculosisare causing the disease in the area while isolation ofM. boviscould suggest its zoonotic potential. Additionally, identification ofM. africanumrequires further confirmation by tools with a better discriminatory power.

scholarly journals Genetic diversity of the Mycobacterium tuberculosis East African–Indian family in three tropical Asian countries

2017 ◽  
Vol 50 (6) ◽  
pp. 886-892 ◽  
Author(s):  
Yih-Yuan Chen ◽  
Jia-Ru Chang ◽  
Wei-Feng Huang ◽  
Chih-Hao Hsu ◽  
Han-Yin Cheng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Asian Countries ◽  
East African ◽  
Indian Family

scholarly journals Genotypes of Mycobacterium tuberculosis isolates circulating in Shaanxi Province, China

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0242971
Author(s):  
Yan Li ◽  
Yu Pang ◽  
Tianhua Zhang ◽  
Xiaoping Xian ◽  
Jian Yang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Multidrug Resistant ◽  
Shaanxi Province ◽  
Drug Resistant ◽  
Beijing Family ◽  
Tuberculosis Patients ◽  
Smear Positive Pulmonary Tuberculosis ◽  
Discriminatory Index

Objectives The prevalence of drug-resistant TB in Shaanxi Province is higher than other areas. This study was aimed to investigate the genetic diversity and epidemiology of Mycobacterium tuberculosis clinical strains in Shaanxi Province, China. Methods From January to December 2016, a total of 298 Mycobacterium tuberculosis clinical isolates from smear-positive pulmonary tuberculosis patients were genotyped by Mcspoligotyping and 15-locus VNTR. Results We found that the Beijing family strains was the most prominent family(81.54%, 243/298). Other family strains included T family(9.06%, 27/298), U family(0.67%, 2/298), LAM9 family(0.34%, 1/298) and Manu family(0.34%, 1/298). The rates of multidrug-resistant (MDR) M.Tuberculosis, age, type of case and education between Beijing and non-Beijing family strains were not statistically different, while the distribution in the three different regions among these was statistically significant. VNTR results showed that strains were classified into 280 genotypes, and 33 (11.07%) strains could be grouped into 14 clusters. 11 of the 15-VNTR loci were highly or moderately discriminative according to the Hunter-Gaston discriminatory index. Conclusions We concluded that the Beijing family genotype was the most prevalent genotype and 15-locus VNTR typing might be suitable for genotyping of M. tuberculosis in Shaanxi Province. There was less association between Beijing family genotypes and drug resistance in our study area.

scholarly journals Mycobacterium tuberculosis lineage 1 genetic diversity in Pará, Brazil, suggests common ancestry with east-African isolates potentially linked to historical slave trade

2019 ◽  
Vol 73 ◽  
pp. 337-341 ◽  
Author(s):  
Emilyn Costa Conceição ◽  
Guislaine Refregier ◽  
Harrison Magdinier Gomes ◽  
Xavier Olessa-Daragon ◽  
Francesc Coll ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Slave Trade ◽  
Common Ancestry ◽  
East African

scholarly journals Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania

Tuberculosis ◽  
2015 ◽  
Vol 95 (2) ◽  
pp. 170-178 ◽  
Author(s):  
Erasto V. Mbugi ◽  
Bugwesa Z. Katale ◽  
Keith K. Siame ◽  
Julius D. Keyyu ◽  
Sharon L. Kendall ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Tuberculosis Patients ◽  
Serengeti Ecosystem

scholarly journals IS6110-based Differentiation of Mycobacterium tuberculosis Strains

2013 ◽  
Vol 62 (2) ◽  
pp. 201-204 ◽  
Author(s):  
ANNA ŻACZEK ◽  
MAŁGORZATA ZIÓŁKIEWICZ ◽  
ARKADIUSZ WOJTASIK ◽  
JAROSŁAW DZIADEK ◽  
ANNA SAJDUDA
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Discriminatory Power ◽  
Specific Method ◽  
Restriction Fragment Length

In this study, 62 Mycobacterium tuberculosis strains were characterized by fast ligation-mediated PCR (FLiP) and, previously performed, IS6110 restriction fragment length polymorphism (RFLP). FLiP proved a reproducible and specific method for differentiation between M. tuberculosis strains. The discriminatory power of FLiP was close to that of the reference IS6110 RFLP suggesting its usefulness in studying the genetic diversity of M. tuberculosis strains.

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