Genetic diversity and drug resistance pattern of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in the Benishangul Gumuz region and its surroundings, Northwest Ethiopia

Objectives The prevalence of drug-resistant TB in Shaanxi Province is higher than other areas. This study was aimed to investigate the genetic diversity and epidemiology of Mycobacterium tuberculosis clinical strains in Shaanxi Province, China. Methods From January to December 2016, a total of 298 Mycobacterium tuberculosis clinical isolates from smear-positive pulmonary tuberculosis patients were genotyped by Mcspoligotyping and 15-locus VNTR. Results We found that the Beijing family strains was the most prominent family(81.54%, 243/298). Other family strains included T family(9.06%, 27/298), U family(0.67%, 2/298), LAM9 family(0.34%, 1/298) and Manu family(0.34%, 1/298). The rates of multidrug-resistant (MDR) M.Tuberculosis, age, type of case and education between Beijing and non-Beijing family strains were not statistically different, while the distribution in the three different regions among these was statistically significant. VNTR results showed that strains were classified into 280 genotypes, and 33 (11.07%) strains could be grouped into 14 clusters. 11 of the 15-VNTR loci were highly or moderately discriminative according to the Hunter-Gaston discriminatory index. Conclusions We concluded that the Beijing family genotype was the most prevalent genotype and 15-locus VNTR typing might be suitable for genotyping of M. tuberculosis in Shaanxi Province. There was less association between Beijing family genotypes and drug resistance in our study area.

Download Full-text

Drug resistance pattern of M. tuberculosis in those who developed the disease after exposure to MDR/XDR pulmonary tuberculosis

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2019-97-12-57-57 ◽

2020 ◽

Vol 97 (12) ◽

pp. 57-57

Author(s):

K. A. Glebov ◽

V. A. Guseva ◽

I. A. Burmistrova ◽

O. V. Lovacheva ◽

A. G. Samoylova ◽

...

Keyword(s):

Drug Resistance ◽

Pulmonary Tuberculosis ◽

Resistance Pattern ◽

Drug Resistance Pattern

Download Full-text

Genetic Diversity ofMycobacterium tuberculosisComplex Isolated from Tuberculosis Patients in Bahir Dar City and Its Surroundings, Northwest Ethiopia

BioMed Research International ◽

10.1155/2015/174732 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

Anwar Nuru ◽

Gezahegne Mamo ◽

Adane Worku ◽

Aschalew Admasu ◽

Girmay Medhin ◽

...

Keyword(s):

Genetic Diversity ◽

Mycobacterium Tuberculosis ◽

Northwest Ethiopia ◽

Discriminatory Power ◽

Geographical Region ◽

Zoonotic Potential ◽

Bahir Dar ◽

East African ◽

Tuberculosis Patients

The knowledge of the diversity of strains ofMycobacterium tuberculosiscomplex (MTBC) species in a specific geographical region can contribute to the control of tuberculosis (TB). This study was conducted to identify the MTBC isolates to the species and spoligotype international type (SIT) level by spoligotyping. A total of 168 MTBC isolates were recovered from TB patients, spoligotyped, and their patterns were compared with those of the strains registered in the SITVIT2 database. Of 168 isolates spoligotyped, 89 patterns were identified. Ninety-eight isolates were clustered into 19 strain groups with clustering percentage of 58.3%. Forty-four strains matched the preexisting SITs in the SITVIT2 database. The dominant strains were SIT289, SIT134, and SIT3411, comprising 16.7% (28/168), 7.14% (12/168), and 4.76% (8/168) of the isolates, respectively. Euro-American (51.2%), East-African-Indian (34.5%), andM. africanum(9.52%) were the major lineages identified. Two strains ofM. boviswere isolated from TB lymphadenitis cases. The high percentage of clustered strains ofM. tuberculosiscould suggest that a small number of lineages ofM. tuberculosisare causing the disease in the area while isolation ofM. boviscould suggest its zoonotic potential. Additionally, identification ofM. africanumrequires further confirmation by tools with a better discriminatory power.

Download Full-text