scholarly journals Serum Metabolic Profiling Analysis of Gout Patients Treated with Traditional Chinese Medicine Tongfengtai Granules Based on Gas Chromatography-Mass Spectrometry

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Jianyong Zhang ◽  
Hong Pan ◽  
Jingjing Xie ◽  
Jing Wang ◽  
Ruyi Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Metabolic Profiling ◽  
Metabolic Pathways ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Before And After ◽  
Metabolic Biomarkers

Gout has become a public health problem that seriously threatens human health. Traditional Chinese medicines (TCMs) have a long history of treating gout and have some advantages compared with the conventional medicines. Compound TCM Tongfengtai granules are gradually being used for clinical treatment of gout, but its mechanism is still unclear. The purpose of this study was to explore the metabolic profiling of serum from gout patients before and after treatment with Tongfengtai granules and identify the differential metabolites and related metabolic pathways. A total of 40 gout patients hospitalized in Shenzhen Traditional Chinese Medicine Hospital from 2018 to March 2019 were recruited in the current study, and serum samples from these patients before and after treatment with Tongfengtai granules were collected. Gas chromatography-mass spectrometry (GC-MS) assay was used to identify serum metabolites. The OPLS-DA VIP method was used to screen for potential metabolic biomarkers, and MetaboAnalyst 4.0 was used to identify related metabolic pathways. The result showed that there was a significant difference in the concentrations of six metabolites in the serum after treatment: D-galactose, lactic acid, 3-hydroxybutyric acid, D-pyran (type) glucose, alanine, and L-isoleucine. Except D-pyran (type) glucose, the serum concentrations of the other five metabolites were all significantly reduced. Besides, pathway enrichment analysis found that these potential metabolic biomarkers were mainly involved in lactose degradation and the glucose-alanine cycle. Thus, the serum metabolic profiling of gout patients treated with Tongfengtai granules changed, and the differential metabolites and related metabolic pathways might provide clues for understanding the mechanism of Tongfengtai granules.

Application of gas chromatography-mass spectrometry in research of traditional Chinese medicine

2009 ◽  
Vol 63 (5) ◽  
Author(s):  
Jiesheng Ye
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Volatile Compounds ◽  
Biological Fluids ◽  
Gas Chromatography Mass Spectrometry ◽  
Isolation And Characterization ◽  
Chromatography Mass Spectrometry ◽  
Low Toxicity

AbstractIt is well known that traditional Chinese medicine (TCM) plays a more and more important role in modern pharmaceutical industry. It has been used in the therapy of many diseases for several thousand years because of its high pharmacological activity, low toxicity and rare side effects. In TCM, as an important group of secondary metabolites, essential oils have attracted a great deal of attention in recent years. Gas chromatography-mass spectrometry (GC-MS) is the most commonly used technique for the analysis of liposoluble constituents, especially volatile/semi-volatile compounds, and their metabolites in biological fluids due to its high resolution, selectivity and sensitivity. This review briefly describes the applications of GC-MS for the isolation and characterization of volatile compounds from TCM. In addition, GC-MS methods adopted in the metabolic profiling of volatile compounds in biological matrices are also described.

scholarly journals metabolic profiling of endophyte-infected and endophyte-free ryegrass grown under sufficient water supply and drought

2007 ◽  
Vol 13 ◽  
pp. 189-189
Author(s):  
H. Hahn ◽  
O. Fiehn ◽  
M.A. Mcmanus ◽  
D.B. Scott
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Drought Stress ◽  
Water Supply ◽  
Metabolic Profiling ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Endophyte Infection

Metabolic profiling using gas-chromatography mass spectrometry was performed for endophyte-infected (E+) and corresponding endophyte-free (E-) clones of two ryegrass genotypes cultivated under sufficient water supply and drought stress. In total, 243 metabolites representing both known and unknown compounds were analysed for samples taken at the end of the drought stress period and after rewatering (n=10 replications per level of genotype, endophyte infection, and water supply).

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