Time-Course Changes of Serum Keratin Concentrations after Liver Transplantation: Contrasting Results of Keratin-18 and Keratin-19 Fragments

Objective. Under normal conditions, adult hepatocytes express only keratin-8 (K8) and keratin-18 (K18), whereas cholangiocytes also express K19. In this study, we delineate the pattern of normal time-course changes in serum K19 and K18 levels after liver transplantation. Patients and Methods. Serum levels of the K19 fragment CYFRA 21-1 and the K18 fragments tissue polypeptide specific antigen (TPS) and M30 (a neoepitope that is generated after caspase cleavage during apoptosis) were measured at baseline and at regular intervals (up to 6 months) after liver transplantation in 11 adult patients. Results. There was a gradual decrease in serum K19 concentrations from baseline values after transplantation, following a time-course pattern similar to that of serum bilirubin. In contrast, serum concentrations of K18 fragments increased markedly shortly after transplantation and gradually decreased thereafter, following a time-course pattern similar to that of serum transaminases. The increase in TPS tended to occur earlier than that in M30, suggesting an initial predominance of hepatocyte necrosis followed by a predominance of apoptosis in the first days after transplantation. Five patients presented posttransplant complications (acute rejection in three cases and HCV recurrence in two cases). An early increase in serum K19 concentrations was observed in all cases. An increase in serum concentrations of K18 fragments (M30 and TPS) was observed in the two cases with HCV recurrence and was more variable in the three cases with acute rejection. Conclusions. Serum concentrations of K19 and K18 fragments follow a dissimilar pattern of time-course changes after liver transplantation. The diagnostic value of variations in these normal patterns should be addressed in future studies.

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Diagnostic value of peripheral blood T-cell activation and soluble IL-2 receptor for acute rejection in liver transplantation

Clinica Chimica Acta ◽

10.1016/s0009-8981(02)00045-1 ◽

2002 ◽

Vol 320 (1-2) ◽

pp. 69-78 ◽

Cited By ~ 19

Author(s):

Andreas Lun ◽

Mi Young Cho ◽

Christian Müller ◽

Gerhard Staffa ◽

Wolf Otto Bechstein ◽

...

Keyword(s):

Liver Transplantation ◽

T Cell ◽

Acute Rejection ◽

T Cell Activation ◽

Peripheral Blood ◽

Cell Activation ◽

Diagnostic Value

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Changes in the serum levels of interleukin-17/interleukin-23 during acute rejection in liver transplantation

Liver Transplantation ◽

10.1002/lt.21724 ◽

2009 ◽

Vol 15 (6) ◽

pp. 629-633 ◽

Cited By ~ 68

Author(s):

Emilio Fábrega ◽

Marcos López-Hoyos ◽

David San Segundo ◽

Fernando Casafont ◽

Fernando Pons-Romero

Keyword(s):

Liver Transplantation ◽

Acute Rejection ◽

Serum Levels ◽

Interleukin 17 ◽

Interleukin 23

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Serum Levels of Interleukin-34 During Acute Rejection in Liver Transplantation

Transplantation Proceedings ◽

10.1016/j.transproceed.2016.08.038 ◽

2016 ◽

Vol 48 (9) ◽

pp. 2977-2979 ◽

Cited By ~ 5

Author(s):

D. San Segundo ◽

P. Ruiz ◽

J. Irure ◽

M.T. Arias-Loste ◽

A. Cuadrado ◽

...

Keyword(s):

Liver Transplantation ◽

Acute Rejection ◽

Serum Levels

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Serum Levels of Interleukin-9 During Acute Rejection in Liver Transplantation

Transplantation Proceedings ◽

10.1016/j.transproceed.2012.05.013 ◽

2012 ◽

Vol 44 (6) ◽

pp. 1533-1535 ◽

Cited By ~ 4

Author(s):

E. Fábrega ◽

M. López-Hoyos ◽

D. San Segundo ◽

F. Casafont ◽

M. Angel Mieses ◽

...

Keyword(s):

Liver Transplantation ◽

Acute Rejection ◽

Serum Levels ◽

Interleukin 9

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Changes of the Serum Antibiotic Levels During Open Heart Surgery (ceftazidim, ciprofloxacin, clindamycin)

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2019.113 ◽

2000 ◽

Vol 43 (1) ◽

pp. 23-27 ◽

Cited By ~ 2

Author(s):

Vladimír Lonský ◽

Jan Dominik ◽

Jiří Manďák ◽

Eugenie Pozlerová ◽

Miroslav Hejzlar ◽

...

Keyword(s):

Time Course ◽

Heart Surgery ◽

Left Internal Mammary Artery ◽

Open Heart Surgery ◽

Sharp Decrease ◽

Serum Levels ◽

Skin Incision ◽

Anesthesia Induction ◽

Open Heart ◽

Serum Concentrations

Background: Wound, mediastinal and intracardiac infections are still very serious complications of open-heart surgey. The incidence of it is still in the range of 0.4%-5%. The aims of our study were to assess the adequacy of regimen using ceftazidim (CTZ), ciprofloxacin (CPF) and clindamycin (CLIN) as prophylactic antibiotics and to verify whether cardiopulmonary bypass (CPB) can modify the time of antibiotic serum concentrations. That is why the serum levels of them were measured during open heart procedures. Methods: The prospective study comprised 75 consequent coronary patients randomized in to three groups receiving 1 g of CTZ or 400 mg of CPF or 900 mg of CLIN i.v. with anesthesia induction. Routine coronary surgery with left internal mammary artery harvesting, moderate body hypothermic (30 °C) CPB with crystaloid cardioplegia was performed. Serum antibiotic levels were determined before application, with skin incision, prior CPB induction, after cardioplegia infusion, every 20 minutes of CPB, prior end of CPB, in time of chest closure. Conventional cylinder – plate microbiological assay was used for antibiotic levelmeasurement. Results: All serum antibiotic concentrations showed a sharp decrease immediately after starting CPB and lasted until CPB ended. After initiating of CPB after cardioplegia administration serum concentrations of CTZ (105 min after initial dose) decreased by, on average 55%, CPF (97 min) by 42% and CLIN (116 min) by 78%. Conclusion: CPB can modify the time course of antibiotic serum concentrations. The serum levels of CTZ at the end of the longest procedures were found to be below the MICs for some of the suspected pathogens. We recommend to use higher antibiotic doses for prophylaxis and to administer the second dose with protamin sulphate to obtain maximum concentration in newly formed blood clots.

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Serum Levels of miR-223-3p and miR-223-5p in Prostate Diseases

MicroRNA ◽

10.2174/2211536609666201106090458 ◽

2020 ◽

Vol 09 ◽

Author(s):

Yakup Dülgeroğlu ◽

Onur Eroğlu

Keyword(s):

Prostate Cancer ◽

Specific Antigen ◽

Serum Levels ◽

Diagnostic Value ◽

Serum Samples ◽

Prostate Hyperplasia ◽

Significant Difference ◽

Prostate Cancer Development ◽

New Biomarkers ◽

Prostate Diseases

Introduction: Prostate cancer (PCa) is the second most common cancer in males and is at fifth place in cancerassociated mortality. Although the prostate-specific antigen (PSA) test is widely used in PCa screenings, it has significant limitations in the differential diagnosis of PCa. Therefore, studies on developing new biomarkers on PCa diagnosis are ongoing. miRNAs are good candidate biomarkers for the diagnosis of cancers, including prostate cancer, as they can be easily detected from the circulation. Objective: In this study, it is aimed to determine diagnostic value of serum levels of miR-223-3p and -223-5p in benign prostate hyperplasia (BPH), chronic prostatitis (CP) and prostate cancer (PCa). Methods: Serum samples was collected from 68 patients in total (25 BPH, 10 CP, 33 PCa). miR-223-3p and -223-5p levels were measured in serum with qRT-PCR. The Ct values of miRNAs were normalized according to the Ct value of ce-miR-39 and calculated -ΔCt values were used statistical analyses. Results: The serum levels of miR-223-3p and -223-5p were downregulated in the PCa and CP groups, compared to the BPH group. There was no statistically significant difference between PCa and CP groups. The sensitivity and specificity of miR-223-3p, -223-5p and their combination were calculated as 88% and 88%; 86% and 79%; 93% and 92% in discriminating BPH and PCa groups, respectively. Conclusion: In this study, it was shown that miR-223-3p and -223-5p were both detectable in the circulation. miR-223- 3p, -223-5p, and their combination may be good candidate biomarkers for prostate cancer diagnosis. Also, observation of similar serum levels of miR-223-3p and -223-5p between CP and PCa groups suggests that miR-223 may play a role in prostate cancer development originated from chronic inflammation.

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