scholarly journals Pharmacological Approaches and Regeneration of Bone Defects with Dental Pulp Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
M. Adamička ◽  
A. Adamičková ◽  
L. Danišovič ◽  
A. Gažová ◽  
J. Kyselovič
Keyword(s):  
Stem Cells ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Marker Genes ◽  
Pharmacological Agents ◽  
Undifferentiated State ◽  
Chondrogenic Marker ◽  
Inflammatory Drugs ◽  
Chemical Stimuli ◽  
Regeneration Of Bone

Bone defects in the craniomaxillofacial skeleton vary from small periodontal defects to extensive bone loss, which are difficult to restore and can lead to extensive damage of the surrounding structures, deformities, and limited functions. Plenty of surgical regenerative procedures have been developed to reconstruct or prevent alveolar defects, based on guided bone regeneration involving the use of autogenous bone grafts or bone substituents. However, these techniques have limitations in the restoration of morphological and functional reconstruction, thus stopping disease progression but not regenerating lost tissue. Most promising candidates for regenerative therapy of maxillofacial bone defects represent postnatal stem cells, because of their replication potential in the undifferentiated state and their ability to differentiate as well. There is an increased need for using various orofacial sources of stem cells with comparable properties to mesenchymal stem cells because they are more easily available with minimally invasive procedures. In addition to the source of MSCs, another aspect affects the regeneration outcomes. Thermal, mechanical, and chemical stimuli after surgical procedures have the ability to generate pain, usually managed with pharmacological agents, mostly nonsteroidal anti-inflammatory drugs (NSAIDs). Some studies revealed that NSAIDs have no significant cytotoxic effect on bone marrow stem cells from mice, while other studies showed regulation of osteogenic and chondrogenic marker genes in MSC cells by NSAIDs and paracetamol, but no effect was observed in connection with diclofenac use. Therefore, there is a need to focus on such pharmacotherapy, capable of affecting the characteristics and properties of implanted MSCs.

scholarly journals Enhanced regeneration of bone defects using sintered porous Ti6Al4V scaffolds incorporated with mesenchymal stem cells and platelet-rich plasma

RSC Advances ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 5128-5138
Author(s):  
Ji Li ◽  
Ketao Wang ◽  
Xiaowei Bai ◽  
Qi Wang ◽  
Ningyu Lv ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Regeneration Of Bone

Porous Ti6AI4V scaffolds incorporated with MSC and PRP are more effective in enhancing the bone regeneration.

scholarly journals Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Bo Wei ◽  
Chengshuo Huang ◽  
Mingyan Zhao ◽  
Peng Li ◽  
Xiang Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Platelet Rich Plasma ◽  
Bone Microarchitecture ◽  
Bone Defects ◽  
Ovariectomized Rats ◽  
Marker Genes ◽  
Osteoporotic Bone ◽  
Specific Marker ◽  
Allogeneic Bone

We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury, in vivo microcomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results. We also assessed changes to bone trabeculae in the proximal tibial growth plate. In ovariectomized rats treated with PRP or with a combination of PRP and BMSCs, the trabecular connectivity densities (Conn.D), bone volume ratios (BV/TV), and numbers (Tb.N) in the defect areas increased significantly from day 7 to day 42. These results indicate that PRP treatment enhances bone microarchitecture in osteoporosis. Moreover, expression levels of osteogenesis-specific marker genes including RUNX2, OSX, and OPN were significantly upregulated in rats treated with PRP and BMSCs compared to those of other groups. Thus, we conclude that treatment with PRP combined with BMSCs significantly promotes healing of osteoporotic bone defects. This study provides an alternative strategy for the treatment of osteoporotic bone loss.

From bench to clinic and back: skeletal stem cells and impaction bone grafting for regeneration of bone defects

2012 ◽  
Vol 8 (10) ◽  
pp. 779-786 ◽  
Author(s):  
A. Aarvold ◽  
J. O. Smith ◽  
E. R. Tayton ◽  
A. M. H. Jones ◽  
J. I. Dawson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Grafting ◽  
Bone Defects ◽  
Impaction Bone Grafting ◽  
Regeneration Of Bone

scholarly journals Effectiveness of mesenchymal stem cell-conditioned medium in bone regeneration in animal and human models: a systematic review and meta-analysis

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Maria Paula Benavides-Castellanos ◽  
Nathaly Garzón-Orjuela ◽  
Itali Linero
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Animal Models ◽  
Conditioned Medium ◽  
Meta Analysis ◽  
Bone Defects ◽  
Favorable Effect ◽  
Human Models ◽  
Regeneration Of Bone

Abstract Background Given the limitations of current therapies for the reconstruction of bone defects, regenerative medicine has arisen as a new therapeutic strategy along with mesenchymal stem cells (MSCs), which, because of their osteogenic potential and immunomodulatory properties, have emerged as a promising alternative for the treatment of bone injuries. In vivo studies have demonstrated that MSCs have a positive effect on regeneration due to their secretion of cytokines and growth factors that, when collected in conditioned medium (MSC-CM) and applied to an injured tissue, can modulate and promote the formation of new tissue. Objective To evaluate the effectiveness of application of conditioned medium derived from mesenchymal stem cells in bone regeneration in animal and human models. Methods We conducted a systematic review with a comprehensive search through February of 2018 using several electronic databases (MEDLINE, EMBASE, SCOPUS, CENTRAL (Ovid), and LILACS), and we also used the “snowballing technique”. Articles that met the inclusion criteria were selected through abstract review and subsequent assessment of the full text. We assessed the risk of bias with the SYRCLE and Cochrane tools, and three meta-analyses were performed. Results We included 21 articles, 19 of which used animal models and 2 of which used human models. In animal models, the application of MSC-CM significantly increased the regeneration of bone defects in comparison with control groups. Human studies reported early mineralization in regenerated bones, and no bone resorption, inflammation, nor local or systemic alterations were observed in any case. The meta-analysis showed an overall favorable effect of the application of MSC-CM. Conclusions The application of MSC-CM to bone defects has a positive and favorable effect on the repair and regeneration of bone tissue, particularly in animal models. It is necessary to perform additional studies to support the application of MSC-CM in clinical practice.

scholarly journals Reconstruction of craniofacial bone defects with autologous human bone marrow stem cells and autogenous bone grafts: A case report with review of literature

2020 ◽  
Vol 12 (5) ◽  
pp. 394
Author(s):  
PrasanthP Panicker ◽  
SunilParamel Mohan ◽  
Jaisanghar Nallusamy ◽  
SumanJ Lakshmi ◽  
Joseph Johny ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Case Report ◽  
Bone Grafts ◽  
Bone Defects ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Autogenous Bone ◽  
Review Of Literature ◽  
Autogenous Bone Grafts

Repair of Critical-Size Bone Defects Using Bone Marrow Stem Cells or Autogenous Bone With or Without Collagen Membrane: A Histomorphometric Study in Rabbit Calvaria

2015 ◽  
Vol 30 (1) ◽  
pp. 208-215 ◽  
Author(s):  
Antonio Aloise ◽  
André Pelegrine ◽  
Allan Zimmermann ◽  
Rafael de Mello e Oliveira ◽  
Lydia Ferreira
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Critical Size ◽  
Bone Defects ◽  
Bone Marrow Stem Cells ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Histomorphometric Study

Hyaluronan initiates chondrogenesis mainly via CD44 in human adipose-derived stem cells

2013 ◽  
Vol 114 (11) ◽  
pp. 1610-1618 ◽  
Author(s):  
Shun-Cheng Wu ◽  
Chung-Hwan Chen ◽  
Je-Ken Chang ◽  
Yin-Chih Fu ◽  
Chih-Kuang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Collagen Type ◽  
Cell Surface Receptor ◽  
Marker Genes ◽  
Adipose Derived Stem Cells ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Sulfated Glycosaminoglycan ◽  
Chondrogenic Marker

Cell-matrix adhesion is one of the important interactions that regulates stem cell survival, self-renewal, and differentiation. Our previous report (Wu SC, Chang JK, Wang CK, Wang GJ, Ho ML. Biomaterials 31: 631–640, 2010) indicated that a microenvironment enriched with hyaluronan (HA) initiated and enhanced chondrogenesis in human adipose-derived stem cells (hADSCs). We further hypothesize that HA-induced chondrogenesis in hADSCs is mainly due to the interaction of HA and CD44 (HA-CD44), a cell surface receptor of HA. The HA-CD44 interaction was tested by examining the mRNA expression of hyaluronidase-1 (Hyal-1) and chondrogenic marker genes (SOX-9, collagen type II, and aggrecan) in hADSCs cultured on HA-coated wells. Cartilaginous matrix formation, sulfated glycosaminoglycan, and collagen productions by hADSCs affected by HA-CD44 interaction were tested in a three-dimensional fibrin hydrogel. About 99.9% of hADSCs possess CD44. The mRNA expressions of Hyal-1 and chondrogenic marker genes were upregulated by HA in hADSCs on HA-coated wells. Blocking HA-CD44 interaction by anti-CD44 antibody completely inhibited Hyal-1 expression and reduced chondrogenic marker gene expression, which indicates that HA-induced chondrogenesis in hADSCs mainly acts through HA-CD44 interaction. A 2-h preincubation and coculture of cells with HA in hydrogel (HA/fibrin hydrogel) not only assisted in hADSC survival, but also enhanced expression of Hyal-1 and chondrogenic marker genes. Higher levels of sulfated glycosaminoglycan and total collagen were also found in HA/fibrin hydrogel group. Immunocytochemistry showed more collagen type II, but less collagen type X, in HA/fibrin than in fibrin hydrogels. Our results indicate that signaling triggered by HA-CD44 interaction significantly contributes to HA-induced chondrogenesis and may be applied to adipose-derived stem cell-based cartilage regeneration.

scholarly journals Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

2017 ◽  
Vol 47 (7) ◽  
Author(s):  
Endrigo Gabellini Leonel Alves ◽  
Rogéria Serakides ◽  
Isabel Rodrigues Rosado ◽  
Omar Leonardo Aristizabal Paez ◽  
Jéssica Alejandra Castro Varon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Bone Formation ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Osteoprogenitor Cells ◽  
Critical Bone Defects

ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.

Gingival mesenchymal stem cells as an alternative source to bone marrow mesenchymal stem cells in regeneration of bone defects: In vivo study

2020 ◽  
Vol 63 ◽  
pp. 101325 ◽  
Author(s):  
Gamilah Al-Qadhi ◽  
Malak Soliman ◽  
Iman Abou-Shady ◽  
Laila Rashed
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defects ◽  
In Vivo Study ◽  
Alternative Source ◽  
Marrow Mesenchymal Stem Cells ◽  
Regeneration Of Bone
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