The Neuroprotective Effect of Carvedilol on Diabetic Neuropathy: An In Vitro Study

Diabetic neuropathy serves as a major complication for diabetic patients across the world. The use of effective treatment is integral for reducing the health complications for diabetic patients. This study has evaluated the carvedilol potential neuroprotective effect on diabetic neuropathy. An in vitro model of diabetic neuropathy was used, including dorsal root ganglia (DRG) that were cultured from male adult mice C57BL. These were incubated for about twenty-four hours in high glucose (HG) media (45 mM). Some cells were incubated with carvedilol (10 μM). Neuronal viability, neuronal morphology, and activating transcription factor 3 (AFT3) were measured. The cell viability was decreased, along with neuronal length, soma area, and soma perimeter with HG media. Also, there was an overexpression of ATF3, which is a neuronal stress response marker. The pretreatment with carvedilol increased the viability of DRG as compared to HG-treated cells. Also, it significantly protected the DRG from HG-induced morphology changes. Though it shows a decrease in AFT3 expression, the statistical results were insignificant. The current study demonstrates the neuroprotective effect of carvedilol against HG-induced DN using an in vitro model. This could be through carvedilol antioxidant effects.

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An in vitro model of diabetic neuropathy: electrophysiological studies

The Neurobiology of Disease ◽

10.1017/cbo9780511570193.008 ◽

1996 ◽

pp. 61-68 ◽

Cited By ~ 2

Author(s):

P. Grafe

Keyword(s):

Diabetic Neuropathy ◽

In Vitro Model ◽

Electrophysiological Studies ◽

Vitro Model

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The neuroprotective effect of cannabidiol in an in vitro model of newborn hypoxic–ischemic brain damage in mice is mediated by CB2 and adenosine receptors

Neurobiology of Disease ◽

10.1016/j.nbd.2009.10.023 ◽

2010 ◽

Vol 37 (2) ◽

pp. 434-440 ◽

Cited By ~ 120

Author(s):

A. Castillo ◽

M.R. Tolón ◽

J. Fernández-Ruiz ◽

J. Romero ◽

J. Martinez-Orgado

Keyword(s):

Brain Damage ◽

Adenosine Receptors ◽

In Vitro Model ◽

Neuroprotective Effect ◽

Ischemic Brain Damage ◽

Ischemic Brain ◽

Vitro Model

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Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis

Experimental Cell Research ◽

10.1016/j.yexcr.2015.12.009 ◽

2016 ◽

Vol 340 (1) ◽

pp. 150-158 ◽

Cited By ~ 61

Author(s):

Roberta Bonafede ◽

Ilaria Scambi ◽

Daniele Peroni ◽

Valentina Potrich ◽

Federico Boschi ◽

...

Keyword(s):

Amyotrophic Lateral Sclerosis ◽

Stromal Cells ◽

In Vitro Model ◽

Neuroprotective Effect ◽

Adipose Derived Stromal Cells ◽

Vitro Model ◽

Lateral Sclerosis

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The neuroprotective effect of rat adipose tissue-derived mesenchymal stem cell-conditioned medium on cortical neurons using an in vitro model of SCI inflammation

Neurological Research ◽

10.1080/01616412.2018.1432266 ◽

2018 ◽

Vol 40 (4) ◽

pp. 258-267 ◽

Cited By ~ 6

Author(s):

Eva Szekiova ◽

Lucia Slovinska ◽

Juraj Blasko ◽

Jana Plsikova ◽

Dasa Cizkova

Keyword(s):

Adipose Tissue ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Cortical Neurons ◽

In Vitro Model ◽

Neuroprotective Effect ◽

Vitro Model ◽

Rat Adipose Tissue ◽

Cell Conditioned Medium

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The Anti-Apoptotic Effect of ASC-Exosomes in an In Vitro ALS Model and Their Proteomic Analysis

Cells ◽

10.3390/cells8091087 ◽

2019 ◽

Vol 8 (9) ◽

pp. 1087 ◽

Cited By ~ 10

Author(s):

Bonafede ◽

Brandi ◽

Manfredi ◽

Scambi ◽

Schiaffino ◽

...

Keyword(s):

Stem Cells ◽

Beneficial Effect ◽

Proteomic Analysis ◽

In Vitro Model ◽

Neuroprotective Effect ◽

Protein Profile ◽

Cell Based Therapy ◽

Apoptotic Effect ◽

Vitro Model

Stem cell therapy represents a promising approach in the treatment of several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). The beneficial effect of stem cells is exerted by paracrine mediators, as exosomes, suggesting a possible potential use of these extracellular vesicles as non-cell based therapy. We demonstrated that exosomes isolated from adipose stem cells (ASC) display a neuroprotective role in an in vitro model of ALS. Moreover, the internalization of ASC-exosomes by the cells was shown and the molecules and the mechanisms by which exosomes could exert their beneficial effect were addressed. We performed for the first time a comprehensive proteomic analysis of exosomes derived from murine ASC. We identified a total of 189 proteins and the shotgun proteomics analysis revealed that the exosomal proteins are mainly involved in cell adhesion and negative regulation of the apoptotic process. We correlated the protein content to the anti-apoptotic effect of exosomes observing a downregulation of pro-apoptotic proteins Bax and cleaved caspase-3 and upregulation of anti-apoptotic protein Bcl-2 α, in an in vitro model of ALS after cell treatment with exosomes. Overall, this study shows the neuroprotective effect of ASC-exosomes after their internalization and their global protein profile, that could be useful to understand how exosomes act, demonstrating that they can be employed as therapy in neurodegenerative diseases.

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Involvement of mitochondria on neuroprotective effect of sphingosine-1-phosphate in cell death in an in vitro model of brain ischemia

Neuroscience Letters ◽

10.1016/j.neulet.2009.12.070 ◽

2010 ◽

Vol 470 (2) ◽

pp. 130-133 ◽

Cited By ~ 24

Author(s):

Alba Agudo-López ◽

Begoña G. Miguel ◽

Inmaculada Fernández ◽

Ana M. Martínez

Keyword(s):

Cell Death ◽

Brain Ischemia ◽

In Vitro Model ◽

Neuroprotective Effect ◽

Sphingosine 1 Phosphate ◽

Vitro Model

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An in vitro model to study diabetic neuropathy

Neuroscience Letters ◽

10.1016/0304-3940(91)90727-b ◽

1991 ◽

Vol 129 (1) ◽

pp. 91-94 ◽

Cited By ~ 13

Author(s):

J.R. Sotelo ◽

H. Horie ◽

S. Ito ◽

C. Benech ◽

K. Sango ◽

...

Keyword(s):

Diabetic Neuropathy ◽

In Vitro Model ◽

Vitro Model

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P.5.103 Neuroprotective effect of a syntheticpeptide mimetic of neural cell adhesion molecule, P2, in an in vitro model of ischemic damage

European Neuropsychopharmacology ◽

10.1016/s0924-977x(05)80505-x ◽

2005 ◽

Vol 15 ◽

pp. S259-S260

Author(s):

K.Y. Voronin ◽

I.V. Lushnikova ◽

G.G. Skibo ◽

V.A. Berezin ◽

E. Bock

Keyword(s):

Cell Adhesion ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

In Vitro Model ◽

Neural Cell Adhesion Molecule ◽

Neuroprotective Effect ◽

Ischemic Damage ◽

Neural Cell Adhesion ◽

Vitro Model

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Microfluidic Organ Chip for In Vitro Model of Blood Glucose Monitoring and Regulation

E3S Web of Conferences ◽

10.1051/e3sconf/202021804029 ◽

2020 ◽

Vol 218 ◽

pp. 04029

Author(s):

Yingqiang Wu ◽

Guo Wu ◽

Pengcheng Fu ◽

Meng Hu

Keyword(s):

Blood Glucose ◽

In Vitro Model ◽

Glucose Monitoring ◽

Genetically Engineered ◽

Blood Glucose Monitoring ◽

Diabetic Patients ◽

Vitro Model ◽

Diabetic Treatment ◽

Near Future

This study is based on our iGEM (international genetically engineered machine) 2019 competition project in which an in vitro model was established to simulate the human monitoring and regulation of blood glucose level using the “liver-on-a-chip” and a genetically engineered bacterium capable of producing proinsulin efficiently. The microfluidic device is able to accommodate cellular chassis loaded with biological parts for diabetic treatment. In addition, electrochemical biosensors were designed to detect the differential glucose concentration from the both chambers of the organ chip. The model can test different chemicals and organs, when the components in the channels and cells are altered. We have thus accomplished an in vitro model of how the proinsulin generated by engineered bacteria works on liver cells. In the near future, our research paradigm will be shifted to bacterial implantation in the human intestines to replace pancreas for the automatic secretion of insulin for diabetic patients.

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